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Deacylated Ghrelin ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA
  • Other names:M46, Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46, GHRL, MTLRP, UNQ524/PRO1066
  • Species:Human, Mouse, Rat
Cat. No. Size Price


YK351 96 wells (1 kit) $759,9
PubMed Product Details
Technical Data

Type

Sandwich ELISA

Applications

Plasma

Sample Requirements

50µL

Shipping

On blue ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store all components of this kit at 2-8°C. This kit is stable under these conditions for 12 months from the date of manufacturing.

Calibration Range

13.6-870 fmol/mL

Limit of Detection

1.36 fmol/mL

Summary

Features

Research Use Only.

This kit measures acylated ghrelin in plasma.

Assay format is 96 wells.

Components of the kit are provided ready-to-use, lyophilized , or concentrated.

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation

Summary

Ghrelin, a novel growth hormone releasing peptide is an acylated peptide that stimulates the release of growth hormone from pituitary. It was isolated from rat stomach and the structure was determined as a peptide consisting of 28-amino acid by Dr. Kenji kangawa (National Cardiovascular Center in Japan). The Ser3 residue of Ghrelin is modified by n-octanoic acid, a modification necessary for hormone activity. This Desacyl-Ghrelin ELISA kit measures only the Desacyl-Ghrelin based on the principle of 2 Site Sandwich enzyme-linked immunosorbent assay (ELISA). It can detect not only human Desacyl-Ghrelin but also rat/mouse Desacyl-Ghrelin (1-28). This kit is manufactured using the high specific antibody pairs generated by Dr. Kangawa and by following his protocol. (patent pending:PCT WO 01/07475 A1)

Product References (6)

References

  • Matsumoto M, Hosoda H, Kitajima Y, Morozumi N, Minamitake Y, Tanaka S, Matsuo H, Kojima M, Hayashi Y, Kangawa K. Structure-activity relationship of ghrelin: pharmacological study of ghrelin peptides. Biochem Biophys Res Commun. 2001 Sep 14;287(1):142-6. doi: 10.1006/bbrc.2001.5553. PubMed PMID: 11549267. See more on PubMed
  • Inui A. Ghrelin: an orexigenic and somatotrophic signal from the stomach. Nat Rev Neurosci. 2001 Aug;2(8):551-60. doi: 10.1038/35086018. PubMed PMID: 11483998. See more on PubMed
  • Gualillo O, Caminos J, Blanco M, Garcìa-Caballero T, Kojima M, Kangawa K, Dieguez C, Casanueva F. Ghrelin, a novel placental-derived hormone. Endocrinology. 2001 Feb;142(2):788-94. doi: 10.1210/endo.142.2.7987. PubMed PMID: 11159851. See more on PubMed
  • Hosoda H, Kojima M, Matsuo H, Kangawa K. Ghrelin and des-acyl ghrelin: two major forms of rat ghrelin peptide in gastrointestinal tissue. Biochem Biophys Res Commun. 2000 Dec 29;279(3):909-13. doi: 10.1006/bbrc.2000.4039. PubMed PMID: 11162448. See more on PubMed
  • Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature. 1999 Dec 9;402(6762):656-60. doi: 10.1038/45230. PubMed PMID: 10604470. See more on PubMed
  • Date Y, Kojima M, Hosoda H, Sawaguchi A, Mondal MS, Suganuma T, Matsukura S, Kangawa K, Nakazato M. Ghrelin, a novel growth hormone-releasing acylated peptide, is synthesized in a distinct endocrine cell type in the gastrointestinal tracts of rats and humans. Endocrinology. 2000 Nov;141(11):4255-61. doi: 10.1210/endo.141.11.7757. PubMed PMID: 11089560. See more on PubMed
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