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Dihydrotestosterone ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Other names:DHT
  • Species:Human
Cat. No. Size Price

RCD009R 96 wells (1 kit) $405,82
PubMed Product Details
Technical Data


Competitive ELISA, Immobilized antigen



Sample Requirements

50 μl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

25–2500 pg/ml

Limit of Detection

17 pg/ml

Intra-assay (Within-Run)

CV = 6.9%

Inter-assay (Run-to-Run)

CV = 8.5%

Spiking Recovery


Dilution Linearity



Research topic

Steroid hormones


5-dihydrotestosterone (DHT) is a steroid similar to testosterone and androstenedione, which belong to a vlase called androgens. DHT is a C19 steroid and possesses androgenic activity. The bulk of androgen production takes place mainly in the Leydig cells of the testes. Androgens circulate in the blood bound to proteins, especially sex hormone binding globulin (SHBG) and albumin. A trace amount of these steroids circulate in the unbound form in the blood and are referred to as the free fractions. DHT has at least three times the binding affinity for SHBG than testosterone. In males about 70% of DHT is derived from peripheral conversion of testosterone, while in females most of the DHT is derived from androstenedione. The major organ to neutralize androgens is the liver. Therefore in the liver the steroid hormones undergo structural modifications that are generally regarded as prerequisites for their biological inactivation. Some metabolites are formed and some are returned to the circulation

Product References (6)


  • Mishra JS, More AS, Kumar S. Elevated androgen levels induce hyperinsulinemia through increase in Ins1 transcription in pancreatic beta cells in female rats. Biol Reprod. 2018 Apr 1;98(4):520-531. doi: 10.1093/biolre/ioy017. PubMed PMID: 29365042. PubMed CentralPMCID: PMC6279097. See more on PubMed
  • Mishra JS, More AS, Hankins GDV, Kumar S. Hyperandrogenemia reduces endothelium-derived hyperpolarizing factor-mediated relaxation in mesenteric artery of female rats. Biol Reprod. 2017 Jun 1;96(6):1221-1230. doi: 10.1093/biolre/iox043. PubMed PMID: 28486649. PubMed CentralPMCID: PMC6279110. See more on PubMed
  • Moon JM, Sung HM, Jung HJ, Seo JW, Wee JH. In vivo evaluation of hot water extract of Acorus gramineus root against benign prostatic hyperplasia. BMC Complement Altern Med. 2017 Aug 22;17(1):414. doi: 10.1186/s12906-017-1887-9. PubMed PMID: 28830494. PubMed CentralPMCID: PMC5568233. See more on PubMed
  • Mishra JS, Hankins GD, Kumar S. Testosterone downregulates angiotensin II type-2 receptor via androgen receptor-mediated ERK1/2 MAP kinase pathway in rat aorta. J Renin Angiotensin Aldosterone Syst. 2016 Oct 19;17(4):1470320316674875. doi: 10.1177/1470320316674875. Print 2016 Oct. PubMed PMID: 27765882. PubMed CentralPMCID: PMC5465964. See more on PubMed
  • Saddick SY. Ovarian surface epithelium receptors during pregnancy and estrus cycle of rats with emphasis on steroids and gonadotropin fluctuation. Saudi J Biol Sci. 2014 Jul;21(3):232-7. doi: 10.1016/j.sjbs.2014.02.004. Epub 2014 Mar 13. PubMed PMID: 24955008. PubMed CentralPMCID: PMC4061401. See more on PubMed
  • Wu YG, Bennett J, Talla D, Stocco C. Testosterone, not 5α-dihydrotestosterone, stimulates LRH-1 leading to FSH-independent expression of Cyp19 and P450scc in granulosa cells. Mol Endocrinol. 2011 Apr;25(4):656-68. doi: 10.1210/me.2010-0367. Epub 2011 Jan 27. PubMed PMID: 21273442. PubMed CentralPMCID: PMC3063088. See more on PubMed
Summary References (14)

References to Dihydrotestosterone

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