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Dihydrotestosterone ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Other names:DHT
  • Species:Human
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Cat. No. Size Price


RCD009R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antigen

Applications

Serum

Sample Requirements

50 μl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

25–2500 pg/ml

Limit of Detection

6.0 pg/ml

Intra-assay (Within-Run)

CV = 6.9%

Inter-assay (Run-to-Run)

CV = 8.5%

Spiking Recovery

90,60%

Dilution Linearity

95,40%

Summary

Research topic

Steroid hormones

Summary

5-dihydrotestosterone (DHT) is a steroid similar to testosterone and androstenedione, which belong to a vlase called androgens. DHT is a C19 steroid and possesses androgenic activity. The bulk of androgen production takes place mainly in the Leydig cells of the testes. Androgens circulate in the blood bound to proteins, especially sex hormone binding globulin (SHBG) and albumin. A trace amount of these steroids circulate in the unbound form in the blood and are referred to as the free fractions. DHT has at least three times the binding affinity for SHBG than testosterone. In males about 70% of DHT is derived from peripheral conversion of testosterone, while in females most of the DHT is derived from androstenedione. The major organ to neutralize androgens is the liver. Therefore in the liver the steroid hormones undergo structural modifications that are generally regarded as prerequisites for their biological inactivation. Some metabolites are formed and some are returned to the circulation

Product References (6)

References

  • Mishra JS, Hankins GD, Kumar S. Testosterone downregulates angiotensin IItype-2 receptor via androgen receptor-mediated ERK1/2 MAP kinase pathway in rataorta. J Renin Angiotensin Aldosterone Syst. 2016 Oct 19;17(4). pii:1470320316674875. Print 2016 Oct. PubMed PMID: 27765882; PubMed Central PMCID:PMC5465964. See more on PubMed
  • Moon JM, Sung HM, Jung HJ, Seo JW, Wee JH. In vivo evaluation of hot waterextract of Acorus gramineus root against benign prostatic hyperplasia. BMCComplement Altern Med. 2017 Aug 22;17(1):414. doi: 10.1186/s12906-017-1887-9.PubMed PMID: 28830494; PubMed Central PMCID: PMC5568233. See more on PubMed
  • Saddick SY. Ovarian surface epithelium receptors during pregnancy and estruscycle of rats with emphasis on steroids and gonadotropin fluctuation. Saudi JBiol Sci. 2014 Jul;21(3):232-7. doi: 10.1016/j.sjbs.2014.02.004. Epub 2014 Mar13. PubMed PMID: 24955008; PubMed Central PMCID: PMC4061401. See more on PubMed
  • Wu YG, Bennett J, Talla D, Stocco C. Testosterone, not 5α-dihydrotestosterone,stimulates LRH-1 leading to FSH-independent expression of Cyp19 and P450scc ingranulosa cells. Mol Endocrinol. 2011 Apr;25(4):656-68. doi:10.1210/me.2010-0367. Epub 2011 Jan 27. PubMed PMID: 21273442; PubMed CentralPMCID: PMC3063088. See more on PubMed
  • Mishra JS, More AS, Kumar S. Elevated androgen levels induce hyperinsulinemia through increase in Ins1 transcription in pancreatic beta cells in female rats.Biol Reprod. 2018 Apr 1;98(4):520-531. doi: 10.1093/biolre/ioy017. PubMed PMID:29365042; PubMed Central PMCID: PMC6279097. See more on PubMed
  • Mishra JS, More AS, Hankins GDV, Kumar S. Hyperandrogenemia reducesendothelium-derived hyperpolarizing factor-mediated relaxation in mesentericartery of female rats. Biol Reprod. 2017 Jun 1;96(6):1221-1230. doi:10.1093/biolre/iox043. PubMed PMID: 28486649; PubMed Central PMCID: PMC6279110. See more on PubMed
Summary References (14)

References to Dihydrotestosterone

  • Bassett RM. A simple chromatographic method for the radioimmunoassay of four androgenic steroids. Med Lab Sci. 1980 Jan;37 (1):31-8
  • Baxendale PM, Jacobs HS, James VH. Plasma and salivary androstenedione and dihydrotestosterone in women with hyperandrogenism. Clin Endocrinol (Oxf). 1983 May;18 (5):447-57
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  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • Dunn JF, Nisula BC, Rodbard D. Transport of steroid hormones: binding of 21 endogenous steroids to both testosterone-binding globulin and corticosteroid-binding globulin in human plasma. J Clin Endocrinol Metab. 1981 Jul;53 (1):58-68
  • Hammond GL, Ruokonen A, Kontturi M, Koskela E, Vihko R. The simultaneous radioimmunoassay of seven steroids in human spermatic and peripheral venous blood. J Clin Endocrinol Metab. 1977 Jul;45 (1):16-24
  • Ito T, Horton R. Dihydrotestosterone in human peripheral plasma. J Clin Endocrinol Metab. 1970 Oct;31 (4):362-8
  • Kricka LJ. Human anti-animal antibody interferences in immunological assays. Clin Chem. 1999 Jul;45 (7):942-56
  • Makin HLJ, Brooks RV. Androgens. Physiology and PathologyIn. Biochemistry of Steroid Hormon. 565:1984.;
  • Mean F, Pellaton M, Magrini G. Study on the binding of dihydrotestosterone, testosterone and oestradiol with sex hormone binding globulin. Clin Chim Acta. 1977 Oct 1;80 (1):171-80
  • Mooradian AD, Morley JE, Korenman SG. Biological actions of androgens. Endocr Rev. 1987 Feb;8 (1):1-28
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