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Dihydrotestosterone ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Other names:DHT
  • Species:Human
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RCD009R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antigen

Applications

Serum

Sample Requirements

50 μl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

25–2500 pg/ml

Limit of Detection

6.0 pg/ml

Intra-assay (Within-Run)

CV = 6.9%

Inter-assay (Run-to-Run)

CV = 8.5%

Spiking Recovery

90,60%

Dilutation Linearity

95,40%

Summary

Research topic

Steroid hormones

Summary

5-dihydrotestosterone (DHT) is a steroid similar to testosterone and androstenedione, which belong to a vlase called androgens. DHT is a C19 steroid and possesses androgenic activity. The bulk of androgen production takes place mainly in the Leydig cells of the testes. Androgens circulate in the blood bound to proteins, especially sex hormone binding globulin (SHBG) and albumin. A trace amount of these steroids circulate in the unbound form in the blood and are referred to as the free fractions. DHT has at least three times the binding affinity for SHBG than testosterone. In males about 70% of DHT is derived from peripheral conversion of testosterone, while in females most of the DHT is derived from androstenedione. The major organ to neutralize androgens is the liver. Therefore in the liver the steroid hormones undergo structural modifications that are generally regarded as prerequisites for their biological inactivation. Some metabolites are formed and some are returned to the circulation

References to Product

References

  • Mishra JS, Hankins GD, Kumar S. Testosterone downregulates angiotensin II type-2 receptor via androgen receptor-mediated ERK1/2 MAP kinase pathway in rat aorta. J Renin Angiotensin Aldosteron. 2016 Oct;17 (4)
  • Saddick SY. Ovarian surface epithelium receptors during pregnancy and estrus cycle of rats with emphasis on steroids and gonadotropin fluctuation. Saudi Journal of Biological Sc. 13 March 2014;
References to Summary

References to Dihydrotestosterone

  • Bassett RM. A simple chromatographic method for the radioimmunoassay of four androgenic steroids. Med Lab Sci. 1980 Jan;37 (1):31-8
  • Baxendale PM, Jacobs HS, James VH. Plasma and salivary androstenedione and dihydrotestosterone in women with hyperandrogenism. Clin Endocrinol (Oxf). 1983 May;18 (5):447-57
  • Cameron EHD. In proceedings of the fifth tenovous workshop. Steroid Immunoassay. 1975;
  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • Dunn JF, Nisula BC, Rodbard D. Transport of steroid hormones: binding of 21 endogenous steroids to both testosterone-binding globulin and corticosteroid-binding globulin in human plasma. J Clin Endocrinol Metab. 1981 Jul;53 (1):58-68
  • Hammond GL, Ruokonen A, Kontturi M, Koskela E, Vihko R. The simultaneous radioimmunoassay of seven steroids in human spermatic and peripheral venous blood. J Clin Endocrinol Metab. 1977 Jul;45 (1):16-24
  • Ito T, Horton R. Dihydrotestosterone in human peripheral plasma. J Clin Endocrinol Metab. 1970 Oct;31 (4):362-8
  • Kricka LJ. Human anti-animal antibody interferences in immunological assays. Clin Chem. 1999 Jul;45 (7):942-56
  • Makin HLJ, Brooks RV. Androgens. Physiology and PathologyIn. Biochemistry of Steroid Hormon. 565:1984.;
  • Mean F, Pellaton M, Magrini G. Study on the binding of dihydrotestosterone, testosterone and oestradiol with sex hormone binding globulin. Clin Chim Acta. 1977 Oct 1;80 (1):171-80
  • Mooradian AD, Morley JE, Korenman SG. Biological actions of androgens. Endocr Rev. 1987 Feb;8 (1):1-28
  • Pazzagli M. Radioimmunoassay of plasma dihydrotestosterone in normal and hypogonadal men. Clin. Endocr. 82:380, 1976;
  • Wakelin K. Relationship of 5ßdihydrotestosterone and 5αdihydrotestosterone to testosetrone in health and disease. J. Endocrinol. 87:450, 1980;
  • Wang C, Wakelin K, White J, Wood PJ. Salivary androgens in hirsutism: are they of use in routine evaluation?. Ann Clin Biochem. 1986 Sep;23 ( Pt 5):590-5
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