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ENDONEXT™ EndoLISA® Endotoxin Detection Assay based on ELISA-technology

  • Regulatory status:RUO
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Cat. No. Size Price


609033 192 tests
Product Details
Technical Data

Description

Hetereogeneous fluorescence microplate assay using a phage-derived capture molecule for the quantitative binding and determination of endotoxins (lipopolysaccharides). The world's first endotoxin detection system based on ELISA-technology. A highly robust method with unprecedented salt and detergent tolerance. Developed and intended for complex sample matrices.

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Unopened kits are stable at 2–8°C until the expiry date printed on the label.

Summary

Features

EndoLISA® is intended for in vitro quantitative determination of lipopolysaccharide (LPS) in aqueous samples and biological fluids in non-regulated fields.

Warning: EndoLISA® is not intended for the diagnosis of human or animal diseases.

Note: EndoLISA® is not suitable for the detection of endotoxin in serum, plasma and blood samples.

  • Overcomes limitations of existing methods, such as the need for extensive dilution
  • Reduced matrix effects due to integrated washing step
  • Robust assay with excellent reproducibility
  • Broad measurement range (0.05–500 EU/ml)
  • Broad pH range (pH4 – pH9)
  • Less interference at high salt conditions e.g. NaCl, GdnHCl
  • All reagents necessary to run the assay included in the kit
  • Uses recombinant Factor C (rFC), thus saving the diminishing horseshoe crab population
Product References (47)

References

  • Amouzou C, Breuker C, Fabre O, Bourret A, Lambert K, Birot O, Fédou C, Dupuy AM, Cristol JP, Sutra T, Molinari N, Maimoun L, Mariano-Goulart D, Galtier F, Avignon A, Stanke-Labesque F, Mercier J, Sultan A, Bisbal C. Skeletal Muscle Insulin Resistance and Absence of Inflammation Characterize Insulin-Resistant Grade I Obese Women. PLoS One. 2016 Apr 25;11(4):e0154119. doi: 10.1371/journal.pone.0154119. PMID: 27111539; PMCID: PMC4844150. See more on PubMed
  • Corbishley A, Ahmad NI, Hughes K, Hutchings MR, McAteer SP, Connelley TK, Brown H, Gally DL, McNeilly TN. Strain-dependent cellular immune responses in cattle following Escherichia coli O157:H7 colonization. Infect Immun. 2014 Dec;82(12):5117-31. doi: 10.1128/IAI.02462-14. Epub 2014 Sep 29. PMID: 25267838; PMCID: PMC4249286. See more on PubMed
  • Dąbrowska K, Miernikiewicz P, Piotrowicz A, Hodyra K, Owczarek B, Lecion D, Kaźmierczak Z, Letarov A, Górski A. Immunogenicity studies of proteins forming the T4 phage head surface. J Virol. 2014 Nov;88(21):12551-7. doi: 10.1128/JVI.02043-14. Epub 2014 Aug 20. PMID: 25142581; PMCID: PMC4248953. See more on PubMed
  • Dhanasooraj D, Kumar RA, Mundayoor S. Vaccine delivery system for tuberculosisbased on nano-sized hepatitis B virus core protein particles. Int J Nanomedicine.2013;8:835-43. doi: 10.2147/IJN.S40238. Epub 2013 Feb 25. PubMed PMID: 23486691; PubMed Central PMCID: PMC3592552. See more on PubMed
  • Eby JC, Gray MC, Hewlett EL. Cyclic AMP-mediated suppression of neutrophil extracellular trap formation and apoptosis by the Bordetella pertussis adenylate cyclase toxin. Infect Immun. 2014 Dec;82(12):5256-69. doi: 10.1128/IAI.02487-14. Epub 2014 Oct 6. PMID: 25287922; PMCID: PMC4249293. See more on PubMed
  • Furuhashi K, Chua YL, Wong KHS, Zhou Q, Lee DCP, Liong KH, Teo GH, Hutchinson PE, Kemeny DM. Priming with high and low respiratory allergen dose induces differential CD4+ T helper type 2 cells and IgE/IgG1 antibody responses in mice. Immunology. 2017 Jun;151(2):227-238. doi: 10.1111/imm.12726. Epub 2017 Apr 3. PMID: 28190273; PMCID: PMC5418460. See more on PubMed
  • Gałuszka A, Stec M, Węglarczyk K, Kluczewska A, Siedlar M, Baran J. Transition Metal Containing Particulate Matter Promotes Th1 and Th17 Inflammatory Response by Monocyte Activation in Organic and Inorganic Compounds Dependent Manner. Int J Environ Res Public Health. 2020 Feb 14;17(4):1227. doi: 10.3390/ijerph17041227. PMID: 32074992; PMCID: PMC7068527. See more on PubMed
  • Garthwaite J, Woodhams PL, Collins MJ, Balazs R. On the preparation of brainslices: morphology and cyclic nucleotides. Brain Res. 1979 Sep 14;173(2):373-7.PubMed PMID: 226214. See more on PubMed
  • Harhala M, Nelson DC, Miernikiewicz P, Heselpoth RD, Brzezicka B, Majewska J, Linden SB, Shang X, Szymczak A, Lecion D, Marek-Bukowiec K, Kłak M, Wojciechowicz B, Lahutta K, Konieczny A, Dąbrowska K. Safety Studies of Pneumococcal Endolysins Cpl-1 and Pal. Viruses. 2018 Nov 15;10(11):638. doi: 10.3390/v10110638. PMID: 30445722; PMCID: PMC6266847. See more on PubMed
  • Hietala V, Horsma-Heikkinen J, Carron A, Skurnik M, Kiljunen S. The Removal of Endo- and Enterotoxins From Bacteriophage Preparations. Front Microbiol. 2019 Jul 23;10:1674. doi: 10.3389/fmicb.2019.01674. PMID: 31396188; PMCID: PMC6664067. See more on PubMed
  • Hodyra-Stefaniak K, Lahutta K, Majewska J, Kaźmierczak Z, Lecion D, Harhala M, Kęska W, Owczarek B, Jończyk-Matysiak E, Kłopot A, Miernikiewicz P, Kula D, Górski A, Dąbrowska K. Bacteriophages engineered to display foreign peptides may become short-circulating phages. Microb Biotechnol. 2019 Jul;12(4):730-741. doi: 10.1111/1751-7915.13414. Epub 2019 Apr 29. PMID: 31037835; PMCID: PMC6559017. See more on PubMed
  • Hodyra-Stefaniak K, Miernikiewicz P, Drapała J, Drab M, Jończyk-Matysiak E, Lecion D, Kaźmierczak Z, Beta W, Majewska J, Harhala M, Bubak B, Kłopot A, Górski A, Dąbrowska K. Mammalian Host-Versus-Phage immune response determines phage fate in vivo. Sci Rep. 2015 Oct 6;5:14802. doi: 10.1038/srep14802. PMID: 26440922; PMCID: PMC4594097. See more on PubMed
  • Hornburg D, Kruse T, Anderl F, Daschkin C, Semper RP, Klar K, Guenther A, Mejías-Luque R, Schneiderhan-Marra N, Mann M, Meissner F, Gerhard M. A mass spectrometry guided approach for the identification of novel vaccine candidates in gram-negative pathogens. Sci Rep. 2019 Nov 22;9(1):17401. doi: 10.1038/s41598-019-53493-8. PMID: 31758014; PMCID: PMC6874673. See more on PubMed
  • Christler A, Felföldi E, Mosor M, Sauer D, Walch N, Dürauer A, Jungbauer A. Semi-automation of process analytics reduces operator effect. Bioprocess Biosyst Eng. 2020 May;43(5):753-764. doi: 10.1007/s00449-019-02254-y. Epub 2019 Dec 7. Erratum in: Bioprocess Biosyst Eng. 2020 Mar 6;: PMID: 31813007; PMCID: PMC7125066. See more on PubMed
  • Khawaja AA, Pericleous C, Ripoll VM, Porter JC, Giles IP. Autoimmune rheumatic disease IgG has differential effects upon neutrophil integrin activation that is modulated by the endothelium. Sci Rep. 2019 Feb 4;9(1):1283. doi: 10.1038/s41598-018-37852-5. PMID: 30718722; PMCID: PMC6361939. See more on PubMed
  • Kubala MH, Punj V, Placencio-Hickok VR, Fang H, Fernandez GE, Sposto R, DeClerck YA. Plasminogen Activator Inhibitor-1 Promotes the Recruitment and Polarization of Macrophages in Cancer. Cell Rep. 2018 Nov 20;25(8):2177-2191.e7. doi: 10.1016/j.celrep.2018.10.082. PMID: 30463014; PMCID: PMC6876299. See more on PubMed
  • Majewska J, Beta W, Lecion D, Hodyra-Stefaniak K, Kłopot A, Kaźmierczak Z, Miernikiewicz P, Piotrowicz A, Ciekot J, Owczarek B, Kopciuch A, Wojtyna K, Harhala M, Mąkosa M, Dąbrowska K. Oral Application of T4 Phage Induces Weak Antibody Production in the Gut and in the Blood. Viruses. 2015 Aug 20;7(8):4783-99. doi: 10.3390/v7082845. PMID: 26308042; PMCID: PMC4576206. See more on PubMed
  • Mayerhofer R, Fröhlich EE, Reichmann F, Farzi A, Kogelnik N, Fröhlich E, Sattler W, Holzer P. Diverse action of lipoteichoic acid and lipopolysaccharide on neuroinflammation, blood-brain barrier disruption, and anxiety in mice. Brain Behav Immun. 2017 Feb;60:174-187. doi: 10.1016/j.bbi.2016.10.011. Epub 2016 Oct 14. PMID: 27751870; PMCID: PMC5419569. See more on PubMed
  • McDonnell T, Pericleous C, Laurine E, Tommasi R, Garza-Garcia A, Giles I, Ioannou Y, Rahman A. Development of a high yield expression and purification system for Domain I of Beta-2-glycoprotein I for the treatment of APS. BMC Biotechnol. 2015 Nov 14;15:104. doi: 10.1186/s12896-015-0222-0. PMID: 26576675; PMCID: PMC4650279. See more on PubMed
  • Miernikiewicz P, Kłopot A, Soluch R, Szkuta P, Kęska W, Hodyra-Stefaniak K, Konopka A, Nowak M, Lecion D, Kaźmierczak Z, Majewska J, Harhala M, Górski A, Dąbrowska K. T4 Phage Tail Adhesin Gp12 Counteracts LPS-Induced Inflammation In Vivo. Front Microbiol. 2016 Jul 14;7:1112. doi: 10.3389/fmicb.2016.01112. PMID: 27471503; PMCID: PMC4943950. See more on PubMed
  • Miura T, Sano D, Suenaga A, Yoshimura T, Fuzawa M, Nakagomi T, Nakagomi O, Okabe S. Histo-blood group antigen-like substances of human enteric bacteria as specific adsorbents for human noroviruses. J Virol. 2013 Sep;87(17):9441-51. doi: 10.1128/JVI.01060-13. Epub 2013 Jun 26. PMID: 23804639; PMCID: PMC3754087. See more on PubMed
  • Moradian H, Roch T, Lendlein A, Gossen M. mRNA Transfection-Induced Activation of Primary Human Monocytes and Macrophages: Dependence on Carrier System and Nucleotide Modification. Sci Rep. 2020 Mar 6;10(1):4181. doi: 10.1038/s41598-020-60506-4. PMID: 32144280; PMCID: PMC7060354. See more on PubMed
  • Mühle M, Lehmann M, Hoffmann K, Stern D, Kroniger T, Luttmann W, Denner J. Antigenic and immunosuppressive properties of a trimeric recombinant transmembrane envelope protein gp41 of HIV-1. PLoS One. 2017 Mar 10;12(3):e0173454. doi: 10.1371/journal.pone.0173454. PMID: 28282446; PMCID: PMC5345815. See more on PubMed
  • Nakata R, Shimada H, Fernandez GE, Fanter R, Fabbri M, Malvar J, Zimmermann P, DeClerck YA. Contribution of neuroblastoma-derived exosomes to the production of pro-tumorigenic signals by bone marrow mesenchymal stromal cells. J Extracell Vesicles. 2017 Jun 25;6(1):1332941. doi: 10.1080/20013078.2017.1332941. PMID: 28717423; PMCID: PMC5505006. See more on PubMed
  • Ottman N, Reunanen J, Meijerink M, Pietilä TE, Kainulainen V, Klievink J, Huuskonen L, Aalvink S, Skurnik M, Boeren S, Satokari R, Mercenier A, Palva A, Smidt H, de Vos WM, Belzer C. Pili-like proteins of Akkermansia muciniphila modulate host immune responses and gut barrier function. PLoS One. 2017 Mar 1;12(3):e0173004. doi: 10.1371/journal.pone.0173004. PMID: 28249045; PMCID: PMC5332112. See more on PubMed
  • Paulovičová E, Paulovičová L, Farkaš P, Karelin AA, Tsvetkov YE, Krylov VB, Nifantiev NE. Importance of Candida Antigenic Factors: Structure-Driven Immunomodulation Properties of Synthetically Prepared Mannooligosaccharides in RAW264.7 Macrophages. Front Cell Infect Microbiol. 2019 Nov 8;9:378. doi: 10.3389/fcimb.2019.00378. PMID: 31788453; PMCID: PMC6856089. See more on PubMed
  • Paulovičová E, Paulovičová L, Hrubiško M, Krylov VB, Argunov DA, Nifantiev NE. Immunobiological Activity of Synthetically Prepared Immunodominant Galactomannosides Structurally Mimicking Aspergillus Galactomannan. Front Immunol. 2017 Oct 13;8:1273. doi: 10.3389/fimmu.2017.01273. PMID: 29081774; PMCID: PMC5645502. See more on PubMed
  • Piehler M, Roeder R, Blessing S, Reich J. Comparison of LAL and rFC Assays- Participation in a Proficiency Test Program between 2014 and 2019. Microorganisms. 2020 Mar 16;8(3):418. doi: 10.3390/microorganisms8030418. PMID: 32188126; PMCID: PMC7143553. See more on PubMed
  • Reich J, Weyer FA, Tamura H, Nagaoka I, Motschmann H. Low Endotoxin Recovery- Masking of Naturally Occurring Endotoxin. Int J Mol Sci. 2019 Feb 15;20(4):838. doi: 10.3390/ijms20040838. PMID: 30781342; PMCID: PMC6412962. See more on PubMed
  • Ripoll VM, Pregnolato F, Mazza S, Bodio C, Grossi C, McDonnell T, Pericleous C, Meroni PL, Isenberg DA, Rahman A, Giles IP. Gene expression profiling identifies distinct molecular signatures in thrombotic and obstetric antiphospholipid syndrome. J Autoimmun. 2018 Sep;93:114-123. doi: 10.1016/j.jaut.2018.07.002. Epub 2018 Jul 19. PMID: 30033000; PMCID: PMC6123515. See more on PubMed
  • Samadi N, Klems M, Heiden D, Bauer R, Kitzmüller C, Weidmann E, Ret D, Ondracek AS, Duschl A, Horejs-Hoeck J, Untersmayr E. Nitrated food proteins induce a regulatory immune response associated with allergy prevention after oral exposure in a Balb/c mouse food allergy model. Allergy. 2020 Feb;75(2):412-422. doi: 10.1111/all.14030. Epub 2019 Oct 1. PMID: 31444907; PMCID: PMC7064937. See more on PubMed
  • Saunders KO, Wang L, Joyce MG, Yang ZY, Balazs AB, Cheng C, Ko SY, Kong WP, Rudicell RS, Georgiev IS, Duan L, Foulds KE, Donaldson M, Xu L, Schmidt SD, Todd JP, Baltimore D, Roederer M, Haase AT, Kwong PD, Rao SS, Mascola JR, Nabel GJ. Broadly Neutralizing Human Immunodeficiency Virus Type 1 Antibody Gene Transfer Protects Nonhuman Primates from Mucosal Simian-Human Immunodeficiency Virus Infection. J Virol. 2015 Aug;89(16):8334-45. doi: 10.1128/JVI.00908-15. PMID: 26041300; PMCID: PMC4524228. See more on PubMed
  • Seijsing J, Yu S, Frejd FY, Höiden-Guthenberg I, Gräslund T. In vivo depletion of serum IgG by an affibody molecule binding the neonatal Fc receptor. Sci Rep. 2018 Mar 23;8(1):5141. doi: 10.1038/s41598-018-23481-5. PMID: 29572538; PMCID: PMC5865129. See more on PubMed
  • Schwarz H, Schmittner M, Duschl A, Horejs-Hoeck J. Residual endotoxin contaminations in recombinant proteins are sufficient to activate human CD1c+ dendritic cells. PLoS One. 2014 Dec 5;9(12):e113840. doi: 10.1371/journal.pone.0113840. PMID: 25478795; PMCID: PMC4257590. See more on PubMed
  • Singh M, Singh P, Vaira D, Amand M, Rahmouni S, Moutschen M. Minocycline attenuates HIV-1 infection and suppresses chronic immune activation in humanized NOD/LtsZ-scidIL-2Rγ(null) mice. Immunology. 2014 Aug;142(4):562-72. doi: 10.1111/imm.12246. PMID: 24409837; PMCID: PMC4107666. See more on PubMed
  • Szermer-Olearnik B, Boratyński J. Removal of endotoxins from bacteriophage preparations by extraction with organic solvents. PLoS One. 2015 Mar 26;10(3):e0122672. doi: 10.1371/journal.pone.0122672. PMID: 25811193; PMCID: PMC4374689. See more on PubMed
  • Tahoun A, Jensen K, Corripio-Miyar Y, McAteer SP, Corbishley A, Mahajan A, Brown H, Frew D, Aumeunier A, Smith DG, McNeilly TN, Glass EJ, Gally DL. Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella. Vet Res. 2015 Feb 19;46:9. doi: 10.1186/s13567-014-0135-2. PMID: 25827709; PMCID: PMC4333180. See more on PubMed
  • Tahoun A, Jensen K, El-Sharkawy H, Gally D, Rizk AM, Ajarem J, Allam A, Mahmoud AM. Inflammasome Activation in Bovine Peripheral Blood-Derived Macrophages Is Associated with Actin Rearrangement. Animals (Basel). 2020 Apr 10;10(4):655. doi: 10.3390/ani10040655. PMID: 32290067; PMCID: PMC7223366. See more on PubMed
  • Toussaint M, Fievez L, Desmet CJ, Pirottin D, Farnir F, Bureau F, Lekeux P. Increased hypoxia-inducible factor 1α expression in lung cells of horses with recurrent airway obstruction. BMC Vet Res. 2012 May 23;8:64. doi: 10.1186/1746-6148-8-64. PMID: 22621400; PMCID: PMC3536633. See more on PubMed
  • van der Aa E, Buschow SI, Biesta PJ, Janssen HL, Woltman AM. The Effect of Chronic Hepatitis B Virus Infection on BDCA3+ Dendritic Cell Frequency and Function. PLoS One. 2016 Aug 16;11(8):e0161235. doi: 10.1371/journal.pone.0161235. PMID: 27529176; PMCID: PMC4987041. See more on PubMed
  • Van Dyke AL, Kemp TJ, Corbel AF, Zhu B, Gao YT, Wang BS, Rashid A, Shen MC, Hildesheim A, Hsing AW, Pinto LA, Koshiol J. Lipopolysaccharide-pathway proteins are associated with gallbladder cancer among adults in Shanghai, China with mediation by systemic inflammation. Ann Epidemiol. 2016 Oct;26(10):704-709. doi: 10.1016/j.annepidem.2016.08.009. Epub 2016 Aug 31. PMID: 27793274; PMCID: PMC5123694. See more on PubMed
  • van Montfoort N, van der Aa E, van den Bosch A, Brouwers H, Vanwolleghem T, Janssen HLA, Javanbakht H, Buschow SI, Woltman AM. Hepatitis B Virus Surface Antigen Activates Myeloid Dendritic Cells via a Soluble CD14-Dependent Mechanism. J Virol. 2016 Jun 24;90(14):6187-6199. doi: 10.1128/JVI.02903-15. PMID: 27099316; PMCID: PMC4936135. See more on PubMed
  • Vandebriel RJ, Vermeulen JP, van Engelen LB, de Jong B, Verhagen LM, de la Fonteyne-Blankestijn LJ, Hoonakker ME, de Jong WH. The crystal structure of titanium dioxide nanoparticles influences immune activity in vitro and in vivo. Part Fibre Toxicol. 2018 Jan 30;15(1):9. doi: 10.1186/s12989-018-0245-5. PMID: 29382351; PMCID: PMC5791356. See more on PubMed
  • Wampach L, Heintz-Buschart A, Fritz JV, Ramiro-Garcia J, Habier J, Herold M, Narayanasamy S, Kaysen A, Hogan AH, Bindl L, Bottu J, Halder R, Sjöqvist C, May P, Andersson AF, de Beaufort C, Wilmes P. Birth mode is associated with earliest strain-conferred gut microbiome functions and immunostimulatory potential. Nat Commun. 2018 Nov 30;9(1):5091. doi: 10.1038/s41467-018-07631-x. PMID: 30504906; PMCID: PMC6269548. See more on PubMed
  • Yang H, Gong Y, Zhang H, Etobayeva I, Miernikiewicz P, Luo D, Li X, Zhang X, Dąbrowska K, Nelson DC, He J, Wei H. ClyJ Is a Novel Pneumococcal Chimeric Lysin with a Cysteine- and Histidine-Dependent Amidohydrolase/Peptidase Catalytic Domain. Antimicrob Agents Chemother. 2019 Mar 27;63(4):e02043-18. doi: 10.1128/AAC.02043-18. PMID: 30642930; PMCID: PMC6437478. See more on PubMed
  • Yang YF, Zhou YD, Hu JC, Luo FL, Xie Y, Shen YY, Bian WX, Yin ZN, Li HL, Zhang XL. Ficolin-A/2, acting as a new regulator of macrophage polarization, mediates the inflammatory response in experimental mouse colitis. Immunology. 2017 Aug;151(4):433-450. doi: 10.1111/imm.12741. Epub 2017 May 15. PMID: 28380665; PMCID: PMC5506452. See more on PubMed
  • Zawawi A, Forman R, Smith H, Mair I, Jibril M, Albaqshi MH, Brass A, Derrick JP, Else KJ. In silico design of a T-cell epitope vaccine candidate for parasitic helminth infection. PLoS Pathog. 2020 Mar 23;16(3):e1008243. doi: 10.1371/journal.ppat.1008243. PMID: 32203551; PMCID: PMC7117776. See more on PubMed
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