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Estradiol ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Species:Human
Cat. No. Size Price

RCD011R 96 wells (1 kit) $388,96
PubMed Product Details
Technical Data


Competitive ELISA, Immobilized antigen


For the quantitative measurement of estradiol in human serum by an ELISA (enzyme-linked immunosorbent assay). This kit is intended for professional use only and is for laboratory use only. For reserch use only. Intended to be used manually but may be adaptable to open automated analyzers. The user is responsible for validating the performance of this kit with any automated analyzers.



Sample Requirements

50 μl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

25–4000 pg/ml

Limit of Detection

8.4 pg/ml

Intra-assay (Within-Run)

CV = 6.1%

Inter-assay (Run-to-Run)

CV = 7.7%



  • RUO
  • calibration range 25-4000 pg/ml
  • limit of detection 8.4 pg/ml
  • intra-assay CV = 6.1%
  • inter-assay CV = 7.7%

Research topic

Steroid hormones


17β-Estradiol, or Estradiol, is predominantly produced by the ovaries in premenopausal females (1, 2), testes in males (3), and in adipose tissue (4) and the adrenal glands (5) of males and females. Males and postmenopausal females have the lowest estradiol concentration levels (6). In non-pregnant, premenopausal females, estradiol concentrations fluctuate during the menstrual cycle with a peak just before ovulation (7). Estradiol remains elevated during the luteal phase, before decreasing to lower concentrations during the follicular phase.

Summary References (4)

References to Estradiol

  • Hawkins RA, Freedman B, Marshall A, Killen E. Oestradiol-17 beta and prolactin levels in rat peripheral plasma. Br J Cancer. 1975 Aug;32 (2):179-85
  • McCarthy MM. Estradiol and the developing brain. Physiol. Rev. 2008;88:91-134
  • Shaikh AA. Estrone and estradiol levels in the ovarian venous blood from rats during the estrous cycle and pregnancy. Biol Reprod. 1971 Dec;5 (3):297-307
  • Zamani MR, Desmond NL, Levy WB. Estradiol modulates long-term synaptic depression in female rat hippocampus. J Neurophysiol. 2000 Oct;84 (4):1800-8
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