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Manufactured by BioVendor

Estradiol ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Species:Human
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Cat. No. Size Price

RCD011R 96 wells (1 kit)
PubMed Product Details
Technical Data


Competitive ELISA, Immobilized antigen



Sample Requirements

50 μl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

20–3200 pg/ml

Limit of Detection

10 pg/ml

Intra-assay (Within-Run)

CV = 7.7%

Inter-assay (Run-to-Run)

CV = 8.7%

Spiking Recovery


Dilution Linearity



Research topic

Steroid hormones


Estradiol is one of the main components of naturally occurring estrogens and is the major estrogen secreted during the menstrual cycle. The serum levels of estradiol are low during the follicular phase rising gradually until about one day before ovulation when a marked rise in the estradiol level occurs (Ovulatory Peak). The estradiol level falls rapidly at, or right after ovulation and is again within the levels of the follicular phase. There is a second rise of estradiol around day 21 of the cycle (Luteal Peak). The levels then decline gradually to the lowest level at the onset of the next menstrual cycle.

Summary References (4)

References to Estradiol

  • Hawkins RA, Freedman B, Marshall A, Killen E. Oestradiol-17 beta and prolactin levels in rat peripheral plasma. Br J Cancer. 1975 Aug;32 (2):179-85
  • McCarthy MM. Estradiol and the developing brain. Physiol. Rev. 2008;88:91-134
  • Shaikh AA. Estrone and estradiol levels in the ovarian venous blood from rats during the estrous cycle and pregnancy. Biol Reprod. 1971 Dec;5 (3):297-307
  • Zamani MR, Desmond NL, Levy WB. Estradiol modulates long-term synaptic depression in female rat hippocampus. J Neurophysiol. 2000 Oct;84 (4):1800-8
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