Type
Competitive ELISA, Immobilized antigen
Applications
Serum
Sample Requirements
50 μl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
20–3200 pg/ml
Limit of Detection
10 pg/ml
Intra-assay (Within-Run)
CV = 7.7%
Inter-assay (Run-to-Run)
CV = 8.7%
Spiking Recovery
104,10%
Dilution Linearity
96,50%
Research topic
Steroid hormones
Summary
Estradiol is one of the main components of naturally occurring estrogens and is the major estrogen secreted during the menstrual cycle. The serum levels of estradiol are low during the follicular phase rising gradually until about one day before ovulation when a marked rise in the estradiol level occurs (Ovulatory Peak). The estradiol level falls rapidly at, or right after ovulation and is again within the levels of the follicular phase. There is a second rise of estradiol around day 21 of the cycle (Luteal Peak). The levels then decline gradually to the lowest level at the onset of the next menstrual cycle.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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