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Estradiol ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Species:Human
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Cat. No. Size Price


RCD011R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antigen

Applications

Serum

Sample Requirements

50 μl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

20–3200 pg/ml

Limit of Detection

10 pg/ml

Intra-assay (Within-Run)

CV = 7.7%

Inter-assay (Run-to-Run)

CV = 8.7%

Spiking Recovery

104,10%

Dilutation Linearity

96,50%

Summary

Research topic

Steroid hormones

Summary

Estradiol is one of the main components of naturally occurring estrogens and is the major estrogen secreted during the menstrual cycle. The serum levels of estradiol are low during the follicular phase rising gradually until about one day before ovulation when a marked rise in the estradiol level occurs (Ovulatory Peak). The estradiol level falls rapidly at, or right after ovulation and is again within the levels of the follicular phase. There is a second rise of estradiol around day 21 of the cycle (Luteal Peak). The levels then decline gradually to the lowest level at the onset of the next menstrual cycle.

References to Summary

References to Estradiol

  • Hawkins RA, Freedman B, Marshall A, Killen E. Oestradiol-17 beta and prolactin levels in rat peripheral plasma. Br J Cancer. 1975 Aug;32 (2):179-85
  • McCarthy MM. Estradiol and the developing brain. Physiol. Rev. 2008;88:91-134
  • Shaikh AA. Estrone and estradiol levels in the ovarian venous blood from rats during the estrous cycle and pregnancy. Biol Reprod. 1971 Dec;5 (3):297-307
  • Zamani MR, Desmond NL, Levy WB. Estradiol modulates long-term synaptic depression in female rat hippocampus. J Neurophysiol. 2000 Oct;84 (4):1800-8
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