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Manufactured by BioVendor

Rat Estradiol ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA
  • Species:Rat
Cat. No. Size Price

RTC009R 96 wells (1 kit) $617,35
PubMed Product Details
Technical Data


Competitive ELISA


The BioVendor Estradiol rat ELISA is a competitive immunoassay for the quantitative measurement of estradiol in rat serum. For research use only. Not for use in diagnostic procedures.



Sample Requirements

75 ul/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

5 – 1280 pg/ml

Limit of Detection

2.5 pg/ml



  • RUO
  • calibration range 5-1280 pg/ml
  • limit of detection 2.5 pg/ml

Research topic

Steroid hormones, Animal studies


Estradiol (E2 or 17β-estradiol) is an estrogenic hormone produced by the ovaries and in smaller amounts by the adrenal cortex and testes. It is the most potent female sexual hormone and is essential for maintaining normal female functions. During the oestrous cycle which can be divided into four phases (proestrus, estrus, metestrus, diestrus) estradiol concentrations rise gradually from metestrus to proestrus and fell to barely detectable levels in estrus. The maximum concentration is reached around mid-day of proestrus (1,2). Apart from its effects on sexual characteristics it has important influence on the growth and development of the brain (3,4). In female rodents, the determination of estradiol is a useful marker in evaluating and monitoring the state of the reproductive functions and pregnancy as well.

Summary References (4)

References to Estradiol

  • Hawkins RA, Freedman B, Marshall A, Killen E. Oestradiol-17 beta and prolactin levels in rat peripheral plasma. Br J Cancer. 1975 Aug;32 (2):179-85
  • McCarthy MM. Estradiol and the developing brain. Physiol. Rev. 2008;88:91-134
  • Shaikh AA. Estrone and estradiol levels in the ovarian venous blood from rats during the estrous cycle and pregnancy. Biol Reprod. 1971 Dec;5 (3):297-307
  • Zamani MR, Desmond NL, Levy WB. Estradiol modulates long-term synaptic depression in female rat hippocampus. J Neurophysiol. 2000 Oct;84 (4):1800-8
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