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Estrone ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Species:Human
Cat. No. Size Price

RCD010R 96 wells (1 kit) $403,41
PubMed Product Details
Technical Data


Competitive ELISA, Immobilized antigen


For the quantitative measurement of Estrone in human serum by an ELISA (Enzyme-Linked Immunosorbent Assay). This kit is intended for professional use only and is for laboratory use only. For research use only. Intended to be used manually but may be adaptable to open automated analyzers. The user is responsible for validating the performance of this kit with any automated analyzers.



Sample Requirements

50 μl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

20–2000 pg/ml

Limit of Detection

14.8 pg/ml



  • RUO
  • calibration range 20-2000pg/ml
  • limit of detection 14.8 pg/ml
  • quality controls

Research topic

Steroid hormones


Estrone is a steroid, a female sex hormone and, with estradiol and estriol, one of the three most important endogenous estrogens. Estrogens are involved in the development of female sex organs and secondary sex characteristics. Before the ovum is fertilized the main action of the estrogens is on the growth and function of the reproductive tract in order to prepare it for the fertilized ovum. During the follicular phase of the menstrual cycle the estrone level shows a slight increase. The production of estrone then increases markedly to peak at around day 13. The peak is of short duration and by day 16 of the cycle levels will be low. A second peak occurs at around day 21 of the cycle and if fertilization does not occur, then the production of estrone decreases.

Product References (1)


  • Zhang C, Schilirò T, Gea M, Bianchi S, Spinello A, Magistrato A, Gilardi G, Di Nardo G. Molecular Basis for Endocrine Disruption by Pesticides Targeting Aromatase and Estrogen Receptor. Int J Environ Res Public Health. 2020 Aug 5;17(16):5664. doi: 10.3390/ijerph17165664. PubMed PMID: 32764486. PubMed CentralPMCID: PMC7459580. See more on PubMed
Summary References (10)

References to Estrone

  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • Dressendorfer RA, Kirschbaum C, Rohde W, Stahl F, Strasburger CJ. Synthesis of a cortisol-biotin conjugate and evaluation as a tracer in an immunoassay for salivary cortisol measurement. J Steroid Biochem Mol Biol. 1992 Dec;43 (7):683-92
  • Folan J, Gosling JP, Finn MF, Fottrell PF. Solid-phase enzymoimmunoassay of estrone in saliva. Clin Chem. 1989 Apr;35 (4):569-72
  • Folan J, Gosling JP, Fottrell PF. Solid-phase enzymoimmunoassay of estrone in serum. Clin Chem. 1988 Sep;34 (9):1843-6
  • Hauptmann H, Paulus B, Kaiser T, Luppa PB. Concepts for the syntheses of biotinylated steroids. Part II: 17beta-estradiol derivatives as immunochemical probes. Bioconjug Chem. 2000 Jul-Aug;11 (4):537-48
  • Kim MH, Hosseinian AH, Dupon C. Plasma levels of estrogens, androgens and progesterone during normal and dexamethasone-treated cycles. J Clin Endocrinol Metab. 1974 Oct;39 (4):706-12
  • Kricka LJ. Human anti-animal antibody interferences in immunological assays. Clin Chem. 1999 Jul;45 (7):942-56
  • Meyer HH, Sauerwein H, Mutayoba BM. Immunoaffinity chromatography and a biotin-streptavidin amplified enzymeimmunoassay for sensitive and specific estimation of estradiol-17 beta. J Steroid Biochem. 1990 Feb;35 (2):263-9
  • Speight AC, Hancock KW, Oakey RE. Non-protein-bound oestrogens in plasma and urinary excretion of unconjugated oestrogens in non-pregnant women. J Endocrinol. 1979 Dec;83 (3):385-91
  • Speroff L et al. Hormone biosynthesis, metabolism, and mechanism of action. Clinical gynecologic endocrino. 1983: 1-41;
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