Type
Recombinant protein
Description
Total 110 AA. MW: 12.2 kDa (calculated). UniProtKB acc.no. Q7Z5A7–2 isoform 2 (Gln26-Ser125). N-terminal His-tag (10 extra AA). Protein identity confirmed by LC-MS/MS.
Amino Acid Sequence
MKHHHHHHASQFLKEGQLAAGTCEIVTLDRDSSQPRRTIARQTARCACRKGQIAGTTRARPACVDARIIKTKQWCDMLPCLEGEGCDLLINRSGWTCTQPGGRIKTTTVS
Source
E. coli
Purity
˃ 90 % by SDS-PAGE
SDS-PAGE Gel
14 % SDS-PAGE separation of Human FAM19A5:
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. Reduced and boiled sample, 2.5 μg/lane
3. Non-reduced and non-boiled sample, 2.5 μg/lane
Endotoxin
< 1.0 EU/µg
Formulation
0.2–0.9 mg/ml in 20 mM Tris buffer, 50 mM NaCl, pH 7.5, 10% (w/v) glycerol – filtered (0.4 μm), frozen
Reconstitution
Defrost at ambient temperature.
Applications
Western blotting, ELISA
Shipping
On ice. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store protein at -80°C. Protein remains stable until the expiry date when stored at -80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
Note
This product is intended for research use only.