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FERRITIN ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA
  • Species:Human
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Cat. No. Size Price


New DKO039 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA

Applications

Serum, Plasma

Sample Requirements

20 µl/well

Shipping

Shipped on ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

0 - 5 - 20 - 100 - 400 - 1000 ng/mL

Limit of Detection

0.04 ng/mL

Intra-assay (Within-Run)

n=15
CV≤ 7.5%

Inter-assay (Run-to-Run)

n=16
CV≤ 8.0%

Spiking Recovery

95.8 - 101.6%

Dilution Linearity

96.8 - 107.4%

Summary

Features

European Union: for in vitro diagnostic use

Rest of the world: for research use only!

The total assay time is less than 1.5 hours

Quantitative determination of ferritin concentration in human serum or plasma

Assay format is 96 wells

Quality Control is ready to use

Calibrators are ready to use

For routine analysis

Research topic

Iron metabolism, Oncology

Summary

Ferritin is a globular protein found mainly in the liver, which can store about 2'250 iron (Fe3+) ions. The ferritin molecule consists of a protein shell (apoferritin) composed of heavy and light subunits, which surrounds a crystalline core containing iron oxide and phosphate. Ferritin is synthesized in the liver, spleen and numerous other body tissues, with major concentrations found in the liver, spleen, bone marrow, and intestinal mucosa The ferritin levels measured have a direct correlation with the total amount of iron stored in the body. If ferritin is high there is iron in excess, which would be excreted in the stool. If ferritin is low there is a risk for lack in iron, which sooner or later could lead to anaemia. In the setting of anaemia, serum ferritin is the most sensitive lab test for iron deficiency anaemia. In contrast, serum ferritin levels are normal or increased in anemia associated with chronic disease. Elevated serum ferritin levels have been observed in acute and chronic liver disease and lymphoid malignancy (leukemia and Hodgkin lymphoma). High serum ferritin levels have also been associated with an elevated risk for myocardial infarction in men. Ferritin is also used as a marker for iron overload disorders, such as haemochromatosis in which the ferritin level may be abnormally raised. Ferritin is an acute-phase reactant, it is often elevated in the course of disease. Free iron is toxic to cells, and the body has an elaborate set of protective mechanisms to bind iron in various tissue compartments. Within cells, iron is stored complexed to protein as ferritin or hemosiderin. Apoferritin binds to free ferrous iron and stores it in the ferric state. Under steady state conditions, the serum ferritin level correlates with total body iron stores; thus, the serum ferritin level is the most convenient laboratory test to estimate iron stores.

Summary References (8)

References to Ferritin

  • Bolton AE, Lee-Own V, McLean RK, Challand GS. Three different radioiodination methods for human spleen ferritin compared. Clin Chem. 1979 Oct;25 (10):1826-30
  • Drysdale JW. Ferritin as a Tumor Marker. J. Clin. Immunoassay. 6/3:234-240., 1983¨;
  • Goldie DJ, Thomas MJ. Measurement of serum ferritin by radioimmunoassay. Ann Clin Biochem. 1978 Mar;15 (2):102-8
  • Gonyea LM, Lamb CM, Sundberg RD, Deinard AS. Comparison of three procedures for isolating human ferritin, for use as a standard in an immunoradiometric assay. Clin Chem. 1976 Apr;22 (4):513-8
  • Linpisarn S, Kricka LJ, Kennedy JH, Whitehead TP. Sensitive sandwich enzyme immunoassay for serum ferritin on microtitre plates. Ann Clin Biochem. 1981 Jan;18 (Pt 1):48-53
  • Luxton AW, Walker WH, Gauldie J, Ali AM, Pelletier C. A radioimmunoassay for serum ferritin. Clin Chem. 1977;23 (4):683-9
  • Ng RH, Brown BA, Valdes R Jr. Three commercial methods for serum ferritin compared and the high-dose "hook effect" eliminated. Clin Chem. 1983 Jun;29 (6):1109-13
  • Vernon AP, et al. Ferritin: A Reliable Assay for Detection of Iron Deficiency. The Ligand Quarterly. 3/4:15-16, 1980
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