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Fibroblast Growth Factor 19 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:FGF-19, UNQ334/PRO533
  • Species:Human
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Cat. No. Size Price


RD191107200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

50 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

12.5–800 pg/ml

Limit of Detection

4.8 pg/ml

Intra-assay (Within-Run)

n = 8; CV = 6.0%

Inter-assay (Run-to-Run)

n = 6; CV = 7.5%

Spiking Recovery

90,60%

Dilutation Linearity

96,30%

Crossreactivity

  • bovine Non-detectable
  • cat Not tested
  • goat Non-detectable
  • hamster Non-detectable
  • dog Not tested
  • horse Yes (recommended dilution 1:3)
  • mouse Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • pig Not tested
  • chicken Not tested
  • human Yes
  • monkey Yes (recommended dilution 1:3)
Summary

Features

  • It is intended for research use only
  • The total assay time is less than 3.5 hours
  • The kit measures FGF-19 in serum and plasma (EDTA, citrate, heparin)
  • Assay format - 96 wells
  • Standard and Quality Controls are recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Energy metabolism and body weight regulation

Summary

Fibroblast growth factors (FGFs) are a large family of small (17-26 kDa) polypeptide growth factors found in organisms ranging from nematodes to humans. The FGF family has at least 22 members in vertebrates and share 13-71% amino acid identity. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. During embryonic development, FGFs have diverse roles in regulating cell proliferation, migration and differentiation. In the adult organism, FGFs are homeostatic factors and function in tissue repair and response to injury.
FGF signaling is mediated through one of four FGF receptors (FGFR1-FGFR4), a complex family of transmembrane receptor tyrosine kinases. FGFR5 has also been described, but lacks the kinase domain and signaling capability. FGFs have a high affinity for heparin sulfate proteoglycans and require heparin sulfate to activate FGF receptors. Although multiple FGFs interact with each of the four FGFRs, a novel fibroblast growth factor FGF-19 exhibits exclusive binding to only one of FGF receptors (FGFR4).
The normal function of FGF-19 has not been resolved, although its role in inner ear development has been suggested. It has been also found that hepatocyte expression of FGF-19 is induced by the transcription factor, farnesoid X receptor (FXR). FXR is a key regulator of cholesterol metabolism through suppression of the catabolic enzyme cyp7a, the first and rate-limiting step in the biosynthesis of bile acids (BA). A recent study found that, in humans, circulating FGF-19 has a diurnal rhythm controlled by the transintestinal BA flux, and that FGF-19 modulates hepatic BA synthesis. Through its systemic effects, circulating FGF-19 may also mediate other known BA-dependent effects on lipid and carbohydrate metabolism.
In transgenic mice expressing human FGF-19, researchers found a significant increased metabolic rate as well as decreased body weight and adiposity. Additionally, resistance to both diet-induced obesity and insulin desensitization were found. Similar responses have been observed when recombinant FGF-19 was injected into the mice. However, it has been shown too, that FGF-19 transgenic mice develop hepatic adrenocarcinomas with age, and recombinant FGF-19 treated mice exhibit proliferation of hepatocytes.

Areas of investigation:
Cholesterol metabolism, Metabolic syndrome

Product References (13)

References

  • Bužga M, Evžen M, Pavel K, Tomáš K, Vladislava Z, Pavel Z, Svagera Z. Effects of the intragastric balloon MedSil on weight loss, fat tissue, lipid metabolism, and hormones involved in energy balance. Obes Surg. 2014 Jun;24(6):909-15. doi:10.1007/s11695-014-1191-4. PubMed PMID: 24488758; PubMed Central PMCID:PMC4022986. See more on PubMed
  • Dostálová I, Kaválková P, Haluzíková D, Lacinová Z, Mráz M, Papezová H,Haluzík M. Plasma concentrations of fibroblast growth factors 19 and 21 inpatients with anorexia nervosa. J Clin Endocrinol Metab. 2008 Sep;93(9):3627-32. doi: 10.1210/jc.2008-0746. Epub 2008 Jun 17. PubMed PMID: 18559909. See more on PubMed
  • Eren F, Kurt R, Ermis F, Atug O, Imeryuz N, Yilmaz Y. Preliminary evidence of a reduced serum level of fibroblast growth factor 19 in patients withbiopsy-proven nonalcoholic fatty liver disease. Clin Biochem. 2012Jun;45(9):655-8. doi: 10.1016/j.clinbiochem.2012.03.019. Epub 2012 Mar 19. PubMedPMID: 22465275. See more on PubMed
  • Gallego-Escuredo JM, Gómez-Ambrosi J, Catalan V, Domingo P, Giralt M, FrühbeckG, Villarroya F. Opposite alterations in FGF21 and FGF19 levels and disturbedexpression of the receptor machinery for endocrine FGFs in obese patients. Int J Obes (Lond). 2015 Jan;39(1):121-9. doi: 10.1038/ijo.2014.76. Epub 2014 May 12.PubMed PMID: 24813368. See more on PubMed
  • Gadaleta RM, Scialpi N, Peres C, Cariello M, Ko B, Luo J, Porru E, Roda A,Sabbà C, Moschetta A. Suppression of Hepatic Bile Acid Synthesis by anon-tumorigenic FGF19 analogue Protects Mice from Fibrosis andHepatocarcinogenesis. Sci Rep. 2018 Nov 21;8(1):17210. doi:10.1038/s41598-018-35496-z. PubMed PMID: 30464200; PubMed Central PMCID:PMC6249240. See more on PubMed
  • Lee KJ, Jang YO, Cha SK, Kim MY, Park KS, Eom YW, Baik SK. Expression ofFibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through theNuclear Factor-κB and c-Jun N-Terminal Kinase Pathways. Gut Liver. 2018 Jul15;12(4):449-456. doi: 10.5009/gnl17443. PubMed PMID: 29699061; PubMed CentralPMCID: PMC6027831. See more on PubMed
  • Li Z, Lin B, Lin G, Wu Y, Jie Y, Li X, Ko B, Chong Y, Luo J. Circulating FGF19closely correlates with bile acid synthesis and cholestasis in patients withprimary biliary cirrhosis. PLoS One. 2017 Jun 1;12(6):e0178580. doi:10.1371/journal.pone.0178580. eCollection 2017. PubMed PMID: 28570655; PubMedCentral PMCID: PMC5453554. See more on PubMed
  • Nielsen S, Svane MS, Kuhre RE, Clausen TR, Kristiansen VB, Rehfeld JF, HolstJJ, Madsbad S, Bojsen-Moller KN. Chenodeoxycholic acid stimulates glucagon-likepeptide-1 secretion in patients after Roux-en-Y gastric bypass. Physiol Rep. 2017Feb;5(3). pii: e13140. doi: 10.14814/phy2.13140. Epub 2017 Feb 14. PubMed PMID:28202805; PubMed Central PMCID: PMC5309580. See more on PubMed
  • van de Wiel SMW, de Waart DR, Oude Elferink RPJ, van de Graaf SFJ. Intestinal Farnesoid X Receptor Activation by Pharmacologic Inhibition of the Organic SoluteTransporter α-β. Cell Mol Gastroenterol Hepatol. 2017 Nov 28;5(3):223-237. doi:10.1016/j.jcmgh.2017.11.011. eCollection 2018 Mar. PubMed PMID: 29675448; PubMed Central PMCID: PMC5904037. See more on PubMed
  • Zhou M, Luo J, Chen M, Yang H, Learned RM, DePaoli AM, Tian H, Ling L. Mousespecies-specific control of hepatocarcinogenesis and metabolism by FGF19/FGF15. JHepatol. 2017 Jun;66(6):1182-1192. doi: 10.1016/j.jhep.2017.01.027. Epub 2017 Feb9. PubMed PMID: 28189755. See more on PubMed
  • Zöhrer E, Alisi A, Jahnel J, Mosca A, Della Corte C, Crudele A, Fauler G,Nobili V. Efficacy of docosahexaenoic acid-choline-vitamin E in paediatric NASH: a randomized controlled clinical trial. Appl Physiol Nutr Metab. 2017Sep;42(9):948-954. doi: 10.1139/apnm-2016-0689. Epub 2017 May 16. PubMed PMID:28511023. See more on PubMed
  • Zhou M, Learned RM, Rossi SJ, Tian H, DePaoli AM, Ling L. Therapeutic FGF19promotes HDL biogenesis and transhepatic cholesterol efflux to preventatherosclerosis. J Lipid Res. 2019 Mar;60(3):550-565. doi: 10.1194/jlr.M089961.Epub 2019 Jan 24. PubMed PMID: 30679232; PubMed Central PMCID: PMC6399511. See more on PubMed
  • Zhou M, Yang H, Learned RM, Tian H, Ling L. Non-cell-autonomous activation of IL-6/STAT3 signaling mediates FGF19-driven hepatocarcinogenesis. Nat Commun. 2017May 16;8:15433. doi: 10.1038/ncomms15433. PubMed PMID: 28508871; PubMed CentralPMCID: PMC5440856. See more on PubMed
Summary References (8)

References to Fibroblast Growth Factor 19

  • Shih DM, Kast-Woelbern HR, Wong J, Xia Y-R, Edwards PA and Lusis AJ: A role for FXR and human FGF-19 in the repression of paraoxonas1 gene expression by bile acids.Journal of Lipid Research 47, 384–392 (2006)
  • Lundasen T, Galman C, Angelin B. and Rudling M: Circulating intestinal fibroblast growth factor 19 has a pronounced diurnal variation and modulates hepatic bile acid synthesis in man.Journal of Internal Medicine 260, 530–536 (2006)
  • Kurose H, Okamoto M, Shimizu M, Bito T, Marcelle C, Noji S and Ohuchi H: FGF-19-FGFR4 signaling elaborates lends induction with the FGF8-L-Maf cascade in the chick embryo.Develop. Growth Differ. 47, 213–223 (2005)
  • Fu L, John LM, Adams SH, Yu XX, Tomlinson E, Renz M, Williams PM, Soriano R, Corpuz R, Moffat B, Vandlen R, Simmons L, Foster J, Stephan JP, Tsai SP, Stewart TA: Fibroblast growth factor 19 increases metabolic rate and reverses dietary and leptin-deficient diabetes.Endocrinology 145, 2594–2603 (2004)
  • Strack AM and Myers RW: Modulation of metabolic syndrome by fibroblast growth factor 19 (FGF-19)? Endocrinology 145, 2591–2593 (2004), Review
  • Holt JA, Luo G, Billin AN, Bisi J, McNeill YY, Kozarsky KF, Donahee M, Wang DY, Mansfield TA, Kliewer SA, Goodwin B and Jones SA: Definition of a novel growth factor-dependent signal cascade for the suppression of bile acid biosynthesis.Genes and Development 17, 1581–1591 (2003)
  • Tomlinson E, Fu L, John L, Hultgren B, Huang X, Renz M, Stephan JP, Tsai SP, Powell-Braxton L, French D and Stewart TA: Transgenic mice expressing human fibroblast grwth factor-19 display increased metabolic rate and decreased adiposity.Endocrinology 143, 1741–1747 (2002)
  • Ornitz DM and Itoh N: Fibroblast growth factors.Genome Biology 2(3), 3005.1–3005.12 (2001), Review
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