Select country set
language
Menu Shopping cart 0,00 Search
Distributed product

FREE PSA ELISA

  • Regulatory status:RUO
  • Type:ELISA
  • Other names:Prostate Specific Antigen, Kallikrein 3, Kallikrein-3, hK-3, hK3, KLK-3, KLK3
  • Species:Human
Please select your region to see available products and prices.
Cat. No. Size Price


New DKO138 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

ELISA

Applications

Serum, Plasma

Sample Requirements

25 µl/well

Shipping

Shipped on ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

0 - 0.75 - 1.5 - 3 - 6 - 12 ng/mL

Limit of Detection

0.1 ng/mL

Intra-assay (Within-Run)

n=10
CV≤ 3.7%

Inter-assay (Run-to-Run)

n=30
CV≤ 7.8%

Spiking Recovery

87.8 - 98.6%

Dilution Linearity

88.6 - 115.0%

Summary

Features

European Union: for in vitro diagnostic use

Rest of the world: for research use only!

The total assay time is less than 2.5 hours

Quantitative determination of free PSA concentration in human serum or plasma

Assay format is 96 wells

Quality Controls (high and low) are ready to use

Calibrators are ready to use

For routine analysis

Research topic

Immune Response, Infection and Inflammation, Oncology

Summary

Prostate cancer is the most frequent type of cancer found in man and is the second cause of death due to cancer in males. Until recently, digital rectal examination (DRE) was frequently used as only diagnostic modality for the detection of early stages of prostate cancer. In the recent years the determination of serum PSA levels has become the most accepted method to improve the diagnostic specificity of DRE. Although PSA is a tissue specific protein and is not solely tumor specific, it has become the most important marker for prostate carcinoma, showing a better specificity than other biochemical markers used in this context (PAP, total alkaline phosphatase, carcinoembryonic antigen, etc.) In 1979, Wang et al. isolated a specific antigen for normal prostate tissue and called this protein PSA. PSA is a 33 kDa serine proteinase. Immunohistological studies have shown that PSA is localized in the cytoplasm of prostate acinar cells, ductal epithelium and in the secretion on the ductal lumina, present in normal, benign hyperplastic and malignant prostate tissues as well metastatic prostate cancer and in seminal plasma. If the structural integrity of the prostate is disturbed and/or the gland size is increased, the amount of PSA in the blood plasma may become elevated. In the blood plasma, most of the PSA forms complexes with various proteinase inhibitors. Only a small fraction of PSA circulates as free inactive PSA. Basically three major forms of PSA can be distinguished, only two of which are immunoreactive. The predominant form of PSA is a complex with α1-antichymotrypsin (ACT-PSA). Inactive free PSA (f-PSA) represents around 10-40% of the immunologically detectable PSA. The total amount of immunoreactive PSA is known as total PSA (t-PSA). PSA complexed with α- 2-macroglobulin cannot be detected by immunological assays and is therefore frequently called occult PSA (o-PSA). Current methods of screening men for prostate cancer utilize the detection of t-PSA. Levels of 4.0 ng/ml or higher are strong indicators of the possibility of prostatic cancer and are an indication for follow-up examinations of the patient. However, elevated serum PSA levels are frequently also attributed to benign prostatic hyperplasia, leading to a high percentage of false positive screening results. A potential solution to this problem involves the determination of free PSA levels. Studies have suggested that the percentage of free PSA is lower in patients with prostate cancer than those with benign prostatic hyperplasia. Thus, the measurement of free serum PSA in conjunction with total PSA, can improve specificity of prostate cancer screening in selected men with elevated total serum PSA levels, which would subsequently reduce unnecessary prostate biopsies with minimal effects on cancer detection rates.

Related Products Docs