Type
Sandwich ELISA
Applications
Serum, Plasma
Sample Requirements
50 µl/well
Shipping
Shipped on ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
0 - 5 - 10 - 25 - 50 - 100 mIU/mL
Limit of Detection
0.17 mIU/mL
Intra-assay (Within-Run)
n=20
CV ≤ 9.7%
Inter-assay (Run-to-Run)
n=10
CV ≤ 10.0%
Spiking Recovery
89.3 - 106.3%
Dilution Linearity
100.1 - 110.9%
Note
The kits are CE-IVD certified and intended for professional use.
Features
European Union: for in vitro diagnostic use
Rest of the world: for research use only!
The total assay time is less than 1.5 hours
Quantitative determination of FSH in human serumor plasma
Assay format is 96 wells
Quality Control is ready to use
Calibrators are ready to use
For routine analysis
Research topic
Reproduction
Summary
Follicle Stimulating hormone (FSH) is a glycoprotein consisting of two subunits with an approximate molecular mass of 35,500 daltons. The α- subunit is similar to other pituitary hormones [luteinizing stimulating hormone (LH), thyroid stimulating hormone (TSH) and chorionic gonadotropin (hCG)] while the β-subunit is unique. The β- subunit confers the biological activity to the molecule. Stimulation by gonadotropin-releasing hormone (GnRH) causes release of FSH, as well as LH, from the pituitary and is transported by the blood to their sites of action, the testes or ovary. In men, FSH acts on the Sertoli cells of the testis, stimulating the synthesis of inhibin, which appears to
specifically inhibit further FSH secretion, and androgen-binding protein. Thus, it indirectly supports spermatogenesis. In women, FSH acts on the granulosa cells of the ovary, stimulatin steroidogensis. All ovulatory menstrual cycles have a characteristic pattern of FSH, as well as LH, secretion. The menstrual cycle is divided into a follicular phase and a luteal phase by the midcycle surge of the gonadotropins (LH and FSH). As the follicular phase progresses, FSH concentration decreases. Near the time ovulation occur, about midcycle, FSH peaks (lesser in
magnitude than LH) to its highest level. The clinical usefulness of the measurement of Follicle Stimulating hormone (FSH) in ascertaining the homeostasis of fertility regulation via the hypothalamic - pituitary - gonadal axis has been well established.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
Product Brochure
CE IVD Assays
Other Documents
Declaration of Conformity
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