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Distributed product

FSH ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA
  • Other names:Follicle Stimulating Hormone, Follicle-Stimulating Hormon
  • Species:Human
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New DKO010 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA

Applications

Serum, Plasma

Sample Requirements

50 µl/well

Shipping

Shipped on ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

0 - 5 - 10 - 25 - 50 - 100 mIU/mL

Limit of Detection

0.17 mIU/mL

Intra-assay (Within-Run)

n=20
CV ≤ 9.7%

Inter-assay (Run-to-Run)

n=10
CV ≤ 10.0%

Spiking Recovery

89.3 - 106.3%

Dilution Linearity

100.1 - 110.9%

Summary

Features

European Union: for in vitro diagnostic use

Rest of the world: for research use only!

The total assay time is less than 1.5 hours
Quantitative determination of FSH in human serumor plasma

Assay format is 96 wells

Quality Control is ready to use

Calibrators are ready to use

For routine analysis

Research topic

Reproduction

Summary

Follicle Stimulating hormone (FSH) is a glycoprotein consisting of two subunits with an approximate molecular mass of 35,500 daltons. The α- subunit is similar to other pituitary hormones [luteinizing stimulating hormone (LH), thyroid stimulating hormone (TSH) and chorionic gonadotropin (hCG)] while the β-subunit is unique. The β- subunit confers the biological activity to the molecule. Stimulation by gonadotropin-releasing hormone (GnRH) causes release of FSH, as well as LH, from the pituitary and is transported by the blood to their sites of action, the testes or ovary. In men, FSH acts on the Sertoli cells of the testis, stimulating the synthesis of inhibin, which appears to specifically inhibit further FSH secretion, and androgen-binding protein. Thus, it indirectly supports spermatogenesis. In women, FSH acts on the granulosa cells of the ovary, stimulatin steroidogensis. All ovulatory menstrual cycles have a characteristic pattern of FSH, as well as LH, secretion. The menstrual cycle is divided into a follicular phase and a luteal phase by the midcycle surge of the gonadotropins (LH and FSH). As the follicular phase progresses, FSH concentration decreases. Near the time ovulation occur, about midcycle, FSH peaks (lesser in magnitude than LH) to its highest level. The clinical usefulness of the measurement of Follicle Stimulating hormone (FSH) in ascertaining the homeostasis of fertility regulation via the hypothalamic - pituitary - gonadal axis has been well established.

Summary References (15)

References to FSH

  • Butt WR, Ryle M, Shirley A. Kinetic studies with 125I-labelled follicle-stimulating hormone. J Endocrinol. 1973 Aug;58 (2):275-87
  • Cohen KL. Metabolic, endocrine, and drug-induced interference with pituitary function tests: a review. Metabolism. 1977 Oct;26 (10):1165-77
  • Jeffcoate SL. The control of testicular function in the adult. Clin Endocrinol Metab. 1975 Nov;4 (3):521-43
  • Jockenhovel F, Khan SA, Nieschlag E. Circulating antibodies to monoclonal immunoglobulins used in a follitropin assay may cause incorrect fertility diagnosis. J Clin Chem Clin Biochem. 1989 Oct;27 (10):825-8
  • Kjeld JM, Harsoulis P, Kuku SF, Marshall JC, Kaufman B, Fraser TR. Infusions of hFSH and hLH in normal men. I. Kinetics of human follicle stimulating hormone. Acta Endocrinol (Copenh). 1976 Feb;81 (2):225-33
  • Knobil E. The neuroendocrine control of the menstrual cycle. Recent Prog Horm Res. 1980;36:53-88
  • Leonard JM, Leach RB, Couture M, Paulsen CA. Plasma and urinary follicle-stimulating hormone levels in oligospermia. J Clin Endocrinol Metab. 1972 Jan;34 (1):209-14
  • Lundy LE, Lee SG, Levy W, Woodruff JD, Wu CH, Abdalla M. The ovulatory cycle. A histologic, thermal, steroid, and gonadotropin correlation. Obstet Gynecol. 1974 Jul;44 (1):14-25
  • Marshall JC. Clinics in endocrinology and metabolism. Investigative procedures. Clin Endocrinol Metab. 1975 Nov;4 (3):545-67
  • Rebar RW, Erickson GF, Yen SS. Idiopathic premature ovarian failure: clinical and endocrine characteristics. Fertil Steril. 1982 Jan;37 (1):35-41
  • Reiter EO, Kulin HE. Suppressed follicle stimulating hormone in men with chorionic gonadotropin secreting testicular tumors. J Clin Endocrinol Metab. 1971 Dec;33 (6):957-61
  • Seth J, Hanning I, Bacon RR, Hunter WM. Progress and problems in immunoassays for serum pituitary gonadotrophins: evidence from the UK external quality assessment schemes, (EQAS) 1980-1988. Clin Chim Acta. 1989 Dec 29;186 (1):67-82
  • Shome B, Parlow AF. Human follicle stimulating hormone (hFSH): first proposal for the amino acid sequence of the alpha-subunit (hFSHa) and first demonstration of its identity with the alpha-subunit of human luteinizing hormone (hLHa). J Clin Endocrinol Metab. 1974 Jul;39 (1):199-202
  • Shome B, Parlow AF. Human follicle stimulating hormone: first proposal for the amino acid sequence of the hormone-specific, beta subunit (hFSHb). J Clin Endocrinol Metab. 1974 Jul;39 (1):203-5
  • Uotila M, Ruoslahti E, Engvall E. Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein. J Immunol Methods. 1981;42 (1):11-5
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