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GAD65 Autoantibody ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Glutamic Acid Decarboxylase, GAD65, Anti-GAD, GADAb, Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
  • Species:Human
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Cat. No. Size Price

RGDE/96R 96 wells (1 kit)
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


In GADAb ELISA, GADAb in patients’ sera, calibrators and controls are allowed to interact with GAD65 coated onto ELISA plate wells.



Sample Requirements

25 ul


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

5–2000 U/ml

Limit of Detection

0.57 U/ml

Intra-assay (Within-Run)

CV = 6.4%

Inter-assay (Run-to-Run)

CV = 5.8%


In the DASP 2005 study the GADAb ELISA kit achieved 98% (n=100) specificity and 92% (n=50) sensitivity.



  • RUO
  • calibration range 5-2000 U/ml
  • limit of detection 0.57 U/ml
  • positive and negative control

Research topic

Autoimmunity, Diabetology - Autoimmunity


The GAD65 autoantibody (GADAb) ELISA kit is intended for use by professional persons only, for the quantitative determination of GADAb in human serum. Autoantibodies to pancreatic beta cell antigens are important serological markers of type 1 diabetes mellitus (type 1 DM). The antigens recognised by these antibodies include insulin, glutamic acid decarboxylase (GAD65 kDa isoform) and the islet cell antigen IA-2 or ICA-512 and zinc transporter 8 (ZnT8).

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