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Human Galectin-3 ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:Galactose specific lectin 3, Mac-2 antigen, 35 kDa lectin, Carbohydrate binding protein 35, Laminin binding protein
  • Species:Human
Cat. No. Size Price

RAF015R 96 wells (1 kit) $721,83
PubMed Product Details
Technical Data


Sandwich ELISA, HRP-labelled antibody


The human Galectin-3 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Galectin-3.


Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, Cell culture supernatant

Sample Requirements

50 µl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

0.47–30 ng/ml

Limit of Detection

0.29 ng/ml

Intra-assay (Within-Run)

CV = 7.5%

Inter-assay (Run-to-Run)

CV = 5.4%

Spiking Recovery


Dilution Linearity




  • RUO
  • calibration range 0.47-30 ng/ml
  • intra-assay CV = 7.5%
  • inter-assay CV = 5.4%
  • limit of detection 0.29 ng/ml

Research topic

Extracellular matrix, Immune Response, Infection and Inflammation


Galectin-3 is a 26kDa B-galactoside-binding protein belonging to the Galectin family, which consists of more than ten members. Galectin-3 is composed of a carboxyl-terminal carbohydrate recognition domain (CRD) and amino-terminal tandem repeats. Galectin-3 normally distributes in epithelia of many organs and various inflammatory cells, including macrophages as well as dendritic cells and Kupfer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation and cell differentiation and through transactivation by viral proteins. Its expression is also affected by neoplastic transformation: upregulation is found in certain types of lymphomas, and thyroid carcinoma, while it is down regulated in other types of malignancies such as colon, breast, ovarian and uterine carcinomas. The expression of Galectin-3 has a strong correlation with the grade and malignant potential of primary brain tumors. Increased Galectin-3 levels have also been noted in human atherosclerotic lesions. These findings suggest that Galectin-3 expression is affected during these physiological and pathological responses. Galectin-3 has been shown to function through both intracellular and extracellular actions. It is a component of heterogeneous nuclear ribonuclear protein (hnRNP), a factor in pre-mRNA splicing and has been found to control cell cycle and prevent T-cell apoptosis through interaction with the Bcl-2 family members. On the other hand, this protein, which is secreted from monocytes/macrophages and epithelial cells has been demonstrated to function as an extracellular molecule in activating various types of cells such as monocytes/macrophages, mast cells, neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions and acts as a novel chemoattractant for monocytes and macrophages.

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