Glucagon-Like Peptide-2 Rat ELISA

Cat. No.	Size	Price
RSCYK140R	96 wells (1 kit)

Technical Data

Type

Competitive ELISA, Immobilized antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

25 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.137–100 ng/ml

Intra-assay (Within-Run)

Plasma - CV (%) 3.1 - 7.2 Serum - CV (%) 3.5 - 8.9

Inter-assay (Run-to-Run)

Plasma - CV (%) 6.7 - 11.5 Serum - CV (%) 7.6 - 13.0

Spiking Recovery

Rat serum 111,2 % Rat plasma 108,7 %

Summary

Research topic

Diabetology - Other Relevant Products, Animal studies

Summary

The proglucagon gene is expressed in both pancreatic A cell and intestinal L cell. Tissue-specific posttranslational processing of proglucagon by the prohormone convertase produced the different proglucagon derived peptides (PGDPs) in both pancreas and intestine. The most notable pancreatic PGDP is glucagon, whereas the L cell produces several structurally related peptides, including glucagon-like peptide 1 (GLP-1) and GLP-2, as well as glicentin and oxyntomodulin, which contain glucagon sequence in their molecules. Among PGDPs, GLP-2 has recently been found to show intestinal epithelial proliferation.

References to Product

References

Drucker, D. J. et al: Induction of intestinal epithelial proliferation by glucagon-like peptide 2. Proc Natl Acad Sci USA 93: 7911 – 7916, 1996
Mojsov S. et al: Preproglucagon gene expression in pancreas and intestine diversifies the level of post-transcriptiona processing. J Biol Chem 261: pp11880 – 11889, 1986
Philippe, J.: Structure and pancreatic expression of the insulin and glucagon gene. Endocr Rev 12: 252 - 271, 199

Docs

Product Datasheet (RUO)

Datasheet PDF (RUO)

Safety Information (RUO)

MSDS (RUO)