Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma, Cell culture supernatant
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
0.027 - 20 ng/ml
Limit of Detection
0.025 ng/m
Intra-assay (Within-Run)
CV < 10%
Inter-assay (Run-to-Run)
CV < 12%
Spiking Recovery
serum 119%, plasma 125.9%, cell culture media 119%
Dilution Linearity
serum 87.08%, plasma 100.2%, cell culture media 98.63%
Specificity
This ELISA antibody pair detects human ApoA2. Other species not determined.
Features
- RUO
- calibration range 0.027 - 20 ng/ml
- limit of detection 0.025 ng/ml
- intra-assay CV <10%
- inter-assay CV < 12%
Summary
BioVendor Human ApoA2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ApoA2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human ApoA2 coated on a 96-well plate. Standards and samples are pipetted into the wells and ApoA2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antihuman ApoA2 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ApoA2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Instructions for Use (RUO)
Instructions for Use (RUO)
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