Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma, Cell culture supernatant
Sample Requirements
2 μl
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
2.458 - 600 ng/ml
Limit of Detection
2.5 ng/ml
Intra-assay (Within-Run)
<10%
Inter-assay (Run-to-Run)
<12%
Spiking Recovery
serum 103.4%, plasma 103.4%, cell culture media 101%
Dilution Linearity
serum 101.3%, plasma 107.2%, cell culture media 125.6%
Specificity
This ELISA antibody pair detects human ApoB100. Other species not determined.
Features
- RUO
- calibration range 2.458 - 600 ng/ml
- limit of detection 2.5 ng/ml
- intra-assay CV <10%
- inter-assay CV < 12%
Summary
The BioVendor Human ApoB100 ELISA kit is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ApoB100 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human ApoB100 coated on a 96well plate. Standards and samples are pipetted into the wells and ApoB100 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ApoB100 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ApoB100 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Instructions for Use (RUO)
Instructions for Use (RUO)
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