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Manufactured by BioVendor

Human BAFF Soluble (Hypersensitive) ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:B cell Activating Factor belonging to the TNF family, TNFSF13B, TNFSF20, TALL-1, BLyS, THANK, ZNTF4,Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, BLyS, B-cell-activating factor, Dendritic cell-derived TNF-like molecule, TNF- and APOL-related leukocyte expressed ligand 1, TALL-1, CD_antigen: CD257
  • Species:Human
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Cat. No. Size Price


New RAG024R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma, Cell culture supernatant

Sample Requirements

4µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

15.6 - 500 pg/ml

Limit of Detection

8 pg/ml

Intra-assay (Within-Run)

n = 16
CV = 5.8%

Inter-assay (Run-to-Run)

n = 4
CV = 10.23%

Spiking Recovery

80 - 116%

Dilution Linearity

92 - 107%

Summary

Features

It is intended for research use only

The total assay time is less than 5 hours

The kit measures BAFFL in serum, plasma and cell cuture supernatants

Assay format is 96 wells

Components of the kit are provided ready to use, concentrated or dried

Research topic

Autoimmunity, Immunology, Oncology

Summary

BAFF (B cell activation factor of the TNF family, also known as BLyS or TALL1) is a key survival factor for peripheral B cells. BAFF is a homotrimeric type II transmembrane protein that can be proteolytically processed by furin to be released as soluble cytokine. Soluble BAFF adopts the classical trimeric form of the TNF-family ligand. However, BAFF has the unique property among the TNF-ligand to assemble as a 60-mer. BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation. Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases, such as Sjögren’s syndrome, Rheumatoid Arthritis, Multiple sclerosis and Systemic Lupus Erythematosus (SLE). BAFF is also increased levels in some lymphoid cancers.

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