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Manufactured by BioVendor


  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:IGFBP-4
  • Species:Human
Cat. No. Size Price

New REH005R 96 wells (1 kit) $503,4
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


Serum, Plasma, Cell culture supernatant


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

0.41-100 ng/ml

Limit of Detection

250 pg/ml

Intra-assay (Within-Run)

CV: <10%

Inter-assay (Run-to-Run)

CV: <12%

Spiking Recovery

serum 93.98%, plasma 94.69%, cell culture media 97.38%

Dilution Linearity

serum 94%, plasma 94%, cell culture media 96%


This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IGFBP-1, IGFBP-2, IGFBP-3, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, GCSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNFbeta, TPO, VEGF.



  • RUO
  • calibration range 0.41-100 ng/ml
  • limit of detection 250 pg/ml
  • intra-assay CV <10%
  • inter-assay CV <12%


The BioVendor Human IGFBP-4 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGFBP-4 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human IGFBP-4 coated on a 96well plate. Standards and samples are pipetted into the wells and IGFBP-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IGFBP-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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