Human IGFBP-6 ELISA

Features

• RUO
• calibration range 82.3-60 000 pg/ml
• limit of detection 150 pg/ml
• intra- assay CV <10%
• inter-assay CV <12%

Summary

IGFBPs (Insulin-like growth factor binding proteins) are found in various body fluids such as blood serum, amniotic fluid, and liquor. They are synthesized in the liver and are produced also by various tumor cell lines and cell types. Human IGFBP6 is an O-glycosylated protein. It is abundant in cerebrospinal fluid and has a marked preferential binding affinity for IGF-2 over IGF-1. Levels of IGFBP6 have been found to be increased in human breast cancer cells treated with estradiol and IGF-1 and may thus contribute to mitogenesis. The BioVendor Human IGFBP-6 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGFBP-6 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human IGFBP-6 coated on a 96well plate. Standards and samples are pipetted into the wells and IGFBP-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IGFBP-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.