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Human MMP-2 ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA
  • Other names:Matrix metalloproteinase-2, Gelatinase A, TBE-1, 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, MMP-2, TBE-1, PEX, MMP2 CLG4A
  • Species:Human
Cat. No. Size Price


New RBL001R 96 wells (1 kit) $526,55
PubMed Product Details
Technical Data

Type

Sandwich ELISA

Applications

Serum, Plasma

Sample Requirements

5 µl/well

Shipping

Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

0.625 - 40 ng/ml

Limit of Detection

0.25 ng/ml

Intra-assay (Within-Run)

n = 8

CV = 2.5%

Inter-assay (Run-to-Run)

n = 8
CV = 5.8%

Spiking Recovery

99.7%

Dilution Linearity

106.8%

Summary

Features

It is intended for research use only

The total assay time is less than 3 hours

The kit measures total MMP-2

Assay format is 96 wells

Quality Controls are human serum based

Standard is a recombinant protein

Components of the kit are provided ready to use, concentrated or dried

Research topic

Bone and cartilage metabolism, Cardiovascular disease, Coronary artery disease, Extracellular matrix, Oncology

Summary

Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases, responsible for the integrity of the basement membrane (BM) via degradation of extracellular matrix and BM components. MMPs are presented in various types of cells, including cardiomyocytes, cerebral neurons, hepatocytes, and many others, thus influencing various processes. MMP-2, also called gelatinase A, is reported to play a crucial intracellular role where it cleaves several sarcomeric proteins inside cardiac myocytes during oxidative stress-induced injury.2 Elevated levels of MMP-2 (combined with MMP-9) are also associated with highly tumorigenic cancers, such as breast, gastric.

Summary References (37)

References to MMP-2

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  • Monteagudo C, Merino MJ, San-Juan J, Liotta LA, Stetler-Stevenson WG. Immunohistochemical distribution of type IV collagenase in normal, benign, and malignant breast tissue. Am J Pathol. 1990 Mar;136(3):585-92. PMID: 2156430; PMCID: PMC1877499. See more on PubMed
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