Type
Sandwich ELISA
Applications
Serum, Plasma
Sample Requirements
10 µl/well
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
0.2 - 10 ng/ml
Limit of Detection
0.05 ng/ml
Intra-assay (Within-Run)
n = 8
CV = 4%
Inter-assay (Run-to-Run)
n = 8
CV = 4%
Spiking Recovery
104.7%
Dilution Linearity
94.7%
Features
It is intended for research use only
The total assay time is less than 3 hours
The kit measures total MMP-3
Assay format is 96 wells
Standard is a recombinant protein
Components of the kit are provided ready to use, concentrated or dried
Research topic
Bone and cartilage metabolism, Cardiovascular disease, Coronary artery disease, Oncology, Pulmonary diseases
Summary
Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases, responsible for the integrity of the basement membrane (BM) via degradation of extracellular matrix and BM components. MMPs are presented in various types of cells, including cardiomyocytes, cerebral neurons, hepatocytes, and many others, thus influencing various processes.
MMP-3, also called stromelysin 1, is reported to be found in the cytosol of dopaminergic cells where its activation under stress conditions contributes to neuronal degradation. In chondrocytes, nuclear MMP-3 is suggested to be involved in the development of inflammatory arthritic diseases. Matrix metalloproteinase-3 (MMP-3) is implicated in the formation of atherosclerotic plaques, and the MMP-3 −1612 5A/6A polymorphism is associated with myocardial infarction (MI) and stable coronary artery disease (CAD).
Instructions for Use (RUO)
Instructions for Use (RUO)
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