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Manufactured by BioVendor

Human S100A6 ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:S100 calcium-binding protein A6, Calcyclin, Prolactin receptor-associated protein, PRA, Growth factor-inducible protein 2A9, MLN 4, CACY
  • Species:Human
Cat. No. Size Price

New REH008R 96 wells (1kit) $571,86
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


Serum, Plasma, Cell culture supernatant


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

0.066- 16 ng/ml

Limit of Detection

0.06 ng/ml

Intra-assay (Within-Run)

CV <10%

Inter-assay (Run-to-Run)

CV <12%

Spiking Recovery

serum 113.2%, plasma 106.3%, cell culture media 129.9%

Dilution Linearity

serum 94.39%, plasma 96.07%, cell culture media 106.4%


This ELISA antibody pair detects human S100A6. Other species not determined.



  • RUO
  • calibration range 0.066-16 ng/ml
  • limit of detection 0.06 ng/ml
  • intra-assay CV <10%
  • inter-assay CV <12%

Research topic

Immune Response, Infection and Inflammation, Neural tissue markers, Oncology, Others


The BioVendor Human S100A6 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human S100A6 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human S100A6 coated on a 96well plate. Standards and samples are pipetted into the wells and S100A6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human S100A6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of S100A6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Summary References (9)

References to S100A6

  • Deloulme JC, Assard N, Mbele GO, Mangin C, Kuwano R, Baudier J. S100A6 and S100A11 are specific targets of the calcium- and zinc-binding S100B protein in vivo. J Biol Chem. 2000 Nov 10;275 (45):35302-10
  • Leclerc E, Fritz G, Weibel M, Heizmann CW, Galichet A. S100B and S100A6 differentially modulate cell survival by interacting with distinct RAGE (receptor for advanced glycation end products) immunoglobulin domains. J Biol Chem. 2007 Oct 26;282 (43):31317-31
  • Lindsey JC, Lusher ME, Anderton JA, Gilbertson RJ, Ellison DW, Clifford SC. Epigenetic deregulation of multiple S100 gene family members by differential hypomethylation and hypermethylation events in medulloblastoma. Br J Cancer. 2007 Jul 16;97 (2):267-74
  • Nedjadi T, Kitteringham N, Campbell F, Jenkins RE, Park BK, Navarro P, Ashcroft F, Tepikin A, Neoptolemos JP, Costello E. S100A6 binds to annexin 2 in pancreatic cancer cells and promotes pancreatic cancer cell motility. Br J Cancer. 2009 Oct 6;101 (7):1145-54
  • Nowotny M, Spiechowicz M, Jastrzebska B, Filipek A, Kitagawa K, Kuznicki J. Calcium-regulated interaction of Sgt1 with S100A6 (calcyclin) and other S100 proteins. J Biol Chem. 2003 Jul 18;278 (29):26923-8
  • Shimamoto S, Takata M, Tokuda M, Oohira F, Tokumitsu H, Kobayashi R. Interactions of S100A2 and S100A6 with the tetratricopeptide repeat proteins, Hsp90/Hsp70-organizing protein and kinesin light chain. J Biol Chem. 2008 Oct 17;283 (42):28246-58
  • Tsoporis JN, Izhar S, Parker TG. Expression of S100A6 in cardiac myocytes limits apoptosis induced by tumor necrosis factor-alpha. J Biol Chem. 2008 Oct 31;283 (44):30174-83
  • van Dieck J, Fernandez-Fernandez MR, Veprintsev DB, Fersht AR. Modulation of the oligomerization state of p53 by differential binding of proteins of the S100 family to p53 monomers and tetramers. J Biol Chem. 2009 May 15;284 (20):13804-11
  • Wei BR, Hoover SB, Ross MM, Zhou W, Meani F, Edwards JB, Spehalski EI, Risinger JI, Alvord WG, Quinones OA, Belluco C, Martella L, Campagnutta E, Ravaggi A, Dai RM, Goldsmith PK, Woolard KD, Pecorelli S, Liotta LA, Petricoin EF, Simpson RM. Serum S100A6 concentration predicts peritoneal tumor burden in mice with epithelial ovarian cancer and is associated with advanced stage in patients. PLoS One. 2009;4 (10):e7670
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