Type
Sandwich ELISA
Applications
Serum, Plasma
Sample Requirements
5 µl/well
Shipping
Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
6.25 - 200 ng/ml
Limit of Detection
1.56 ng/ml
Intra-assay (Within-Run)
n = 8
CV = 9.5%
Inter-assay (Run-to-Run)
n = 8
CV =10.5%
Spiking Recovery
95%
Dilution Linearity
110.2%
Features
It is intended for research use only
The total assay time is less than 3 hours
The kit measures specifically SAA1+SAA2 and there is no reactivity with (unsolicited) SAA3or SAA4
Assay format is 96 wells
Quality Controls are human serum based
Standard is a recombinant protein
Components of the kit are provided ready to use, concentrated or dried
Research topic
Coronary artery disease, Immune Response, Infection and Inflammation, Oncology, Transplantation
Summary
Serum amyloid A (SAA) is a highly sensitive acute phase reactant that has been linked to inflammatory diseases, both infectious and non-infectious origin. SAA is an apolipoprotein that interacts with HDL, promotes the accumulation of leukocytes at the site of inflammation and the adhesion of platelets, and participates in the removal of damaged cell membranes. The acute phase SAAs (SAA1, SAA2) are transcriptionally regulated in hepatocytes by a variety of inflammatory cytokines, and they can transiently increase > 1000-fold. The combination of SAA, PCT, and CRP can improve the differential diagnosis of early bacterial and viral infections. Several studies indicated that SAA allows monitoring of disease activity in various inflammatory rheumatic diseases and autoinflammatory diseases, including SLE, amyloidosis, or rheumatoid arthritis. SAA can be also used to risk prediction of coronary heart disease, the prognosis of tumour patients, and observation of transplant rejection.
Instructions for Use (RUO)
Instructions for Use (RUO)
Find documents for the lot
