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Manufactured by BioVendor

Human Tie-1 ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Tie-1
  • Species:Human
Cat. No. Size Price

New REH009R 96 wells (1kit) $518,5
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


Serum, Cell culture supernatant


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

0.68-500 ng/ml

Limit of Detection

600 pg/ml

Intra-assay (Within-Run)

CV <10%

Inter-assay (Run-to-Run)

CV <12%

Spiking Recovery

serum 97%, cell culture media 105.9%

Dilution Linearity

serum 112.5%, cell culture media 113.2%


This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFNgamma, Example Version 7 ENG.001.A 7 / 10 Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.



  • RUO
  • calibration range 0.68-500 ng/ml
  • limit of detection 600 pg/ml
  • intra-assay CV <10%
  • inter-assay CV <12%


The BioVendor Human Tie-1 (Tyrosine Kinase with Ig and EGF homology domain 1) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Tie-1 in serum (human Tie-1 concentration is low in normal serum and may not be detectable in this assay; plasma is not recommended) and cell culture supernatants. This assay employs an antibody specific for human Tie-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Tie-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Tie-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Tie-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm

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