Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Cell culture supernatant
Sample Requirements
50 µl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
31.3–2000 pg/ml
Limit of Detection
9.9 pg/ml
Intra-assay (Within-Run)
CV = 5.0%
Inter-assay (Run-to-Run)
CV = 0.1%
Spiking Recovery
85,00%
Dilution Linearity
116,00%
Research topic
Cardiovascular disease, Coronary artery disease, Cytokines and chemokines and related molecules, Diabetology - Other Relevant Products, Immune Response, Infection and Inflammation, Neural tissue markers, Oncology, Transplantation, Animal studies
Summary
IFNγ is a homodimeric glycoprotein containing approximately 21 to 24 kD subunits. Production of IFNg is a function of T cells and NK cells. IFNγ is produced during infection by T cells of the cytotoxic/suppressor phenotype (CD8) and by a subtype of helper T cells, the Th1 cells. IFNγ is a diagnostic tool for distinguishing tuberculous from other nontuberculous ascites. It is important in the design and monitoring of immunotherapy for multiple sclerosis.