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Distributed product


  • Regulatory status:RUO
  • Type:Sandwich ELISA
  • Other names:IgE
  • Species:Human
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Cat. No. Size Price

DKO060 96 wells (1 kit)
PubMed Product Details
Technical Data


Sandwich ELISA


Serum, Plasma

Sample Requirements

25 µl/well


Shipped on ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

0 - 5 - 25 - 50 - 150 - 400 IU/mL

Limit of Detection

0.27 µg/ml

Intra-assay (Within-Run)

CV≤ 7.2%

Inter-assay (Run-to-Run)

CV≤ 7.6%

Spiking Recovery

93 - 106%


The kits are CE-IVD certified and intended for professional use.



European Union: for in vitro diagnostic use

Rest of the world: for research use only!

The total assay time is less than 1.5 hours
Quantitative determination of total IgE in human serum or plasma

Assay format is 96 wells

Calibrators are ready to use

For routine analysis

Research topic

Asthma and allergic rhinitis, Immunology


Immunoglobulin E (IgE) is an antibody isotypes, found only in mammals. Although IgE is typically the least abundant isotype - blood serum IgE levels in a normal (""non-atopic"") individual are ~150ng/mL, compared to 10mg/mL for the IgGs (the isotypes responsible for most of the classical adaptive immune response) - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from an allergy known as type 1 hypersensitivity. IgE plays an important role in allergy, and in the immune system’s recognition of cancer. People who suffer from true IgE-mediated allergies can have up to 10 times the normal level of IgE in their blood (as do sufferers of hyper-IgE syndrome). The IgE molecules (MW 200,000) bind to the surface of the mast cells and basophilic granulocytes. Subsequently the binding of allergen to cell-bound IgE causes these cells to release histamines and other vasoattive substances. The release of histamines in the body results initiates what is commonly known as an allergic reaction. IgE levels show a slow increase during childhood, reaching adult levels in the second decade of life. In general, the total IgE levels increase with the allergies a person has and the number of times of exposure to the relevant allergens. Significant elevations may be seen in the sensitised individuals, but also in cases of myeloma, pulmonary aspergillosi, and during the active stages of parasitic infections. The measurement of immunoglobulin E (IgE) in serum is widely used in the diagnosis of allergic reactions and parasitic infections. Before making any therapeutic determination it is important, however, to know whether the allergic reaction is IgE mediated or non-IgE mediated. Measurement of total IgE in serum sample, along with other supporting diagnostic information, can help to make that determination.

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