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Immunoglobulin E Mouse ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Species:Mouse
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Cat. No. Size Price

RSHAKRIE010R 96 wells (1 kit)
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


Serum, Plasma

Sample Requirements

5 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

1–100 ng/ml

Limit of Detection

50 pg/ml


Research topic

Animal studies


IgE (Immunoglobulin E) is a glycoprotein with a molecular size of 190 kDa composed of 2 light chains and 2 heave chains (H ). In electrophoresis, it moves to γ1 region. Its biological half life is about 3 days, and its blood level in normal human subject is very low, about 300 ng/ml. The blood level of IgE increases markedly in parasite infection and in hay fever. IgE that is responsible for allergy has been called “reagin”. Sensitization by an allergen increases reagin IgE which binds to FcεR1 receptor in basophilic leucocytes and mast cells at Fc region and sensitizes those cells. If the allergen binds the sensitized cells, those cells will be degranulated and release histamin, serotonin, protease, heparin, chemotactic factor, prostaglandins, leucotriens, and so on, causing bronchoconstriction, mucous edema, and hypersecretion, and leads to type I allergic reactions like bronchial asthma, hives, allergic rhinitis, anaphylaxis, and so on. This is the assay kit for total mouse IgE. Shibayagi is also providing assay kits for allergen(OVA)-specific mouse IgE.

References to Product


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  • Okamoto N, Murata T, Tamai H, Tanaka H, Nagai H. Effects of alpha tocopherol and probucol supplements on allergen-induced airway inflammation and hyperresponsiveness in a mouse model of allergic asthma. Int Arch Allergy Immunol. 2006;141 (2):172-80
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  • Park EJ, Park KC, Eo H, Seo J, Son M, Kim KH, Chang YS, Cho SH, Min KU, Jin M, Kim S. Suppression of spontaneous dermatitis in NC/Nga murine model by PG102 isolated from Actinidia arguta. J Invest Dermatol. 2007 May;127 (5):1154-60
  • Pokharel YR, Lim SC, Kim SC, Heo TH, Choi HK, Kang KW. Sopungyangjae-Tang Inhibits Development of Dermatitis in Nc/Nga Mice. Evid Based Complement Alternat. 2008 Jun;5 (2):173-180
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  • Yuda H, Adachi Y, Taguchi O, Gabazza EC, Hataji O, Fujimoto H, Tamaki S, Nishikubo K, Fukudome K, D'Alessandro-Gabazza CN, Maruyama J, Izumizaki M, Iwase M, Homma I, Inoue R, Kamada H, Hayashi T, Kasper M, Lambrecht BN, Barnes PJ, Suzuki K. Activated protein C inhibits bronchial hyperresponsiveness and Th2 cytokine expression in mice. Blood. 2004 Mar 15;103 (6):2196-204
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