Select country change
Shopping cart (0 , 0,00 ) Menu Search
Manufactured by BioVendor

Interleukin-1 Receptor Antagonist Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:IL-1RA
  • Species:Human
Please select your region to see available products and prices.
Cat. No. Size Price


RAF052R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, Cell culture supernatant

Sample Requirements

50 ul/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

109–7000 pg/ml

Limit of Detection

30 pg/ml

Intra-assay (Within-Run)

CV = 7.2%

Inter-assay (Run-to-Run)

CV = 7.4%

Summary

Research topic

Cytokines and chemokines and related molecules

Summary

Interleukin 1 receptor antagonist (IL-1RA) is a protein related to the IL-1 family based on its similarities in amino acid sequence to both IL-1 beta and IL-1 alpha, similarities in gene structure, and collective gene localization. The human gene contains four exons and maps to chromosome 2q13–14. IL-1 induces the release of prostaglandins, nitric oxide and the synthesis of chemokines that promote cells of the immune system to the site of infection. IL-1 RA binds to the IL-1 receptor and blocks IL-1 action through competitive inhibition. In contrast to IL-1, the binding of IL-1RA does not initiate signaling events. The secretion of IL-1 RA is mediated by a classical signal sequence while neither IL-1α nor IL-1β possess such a sequence. The lack of homology between IL-1 RA and IL-1 alpha or IL-1 beta near the N terminus may provide an explanation for the biosynthetic and functional differences between IL-1 RA and the two IL-1 proteins. The production of IL-1RA is stimulated by many substances including adherent IgG, cytokines, and bacterial or viral components. Triggering IL-1 production through cytokines like IL-13, IL-6, IL-4, IFNγ, GM-CSF and TGFβ apparently triggers IL-1RA synthesis. A number of cell types express IL-1RA, including monocytes, macrophages, neutrophils, Sertoli cells, hepatocytes, adipocytes, synovial fibroblasts, mast cells, pancreatic cells and intestinal epithelial cells. IL-1RA is an important natural anti-inflammatory protein that when missing or just available in inadequate amounts plays a key role in several pathological conditions such as rheumatoid arthritis, colitis, osteomyelitis, periostitis and pustulosis. A polymorphism of the IL –1RA gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. In healthy tissue sufficient IL-1RA is produced to hold interleukin-1-mediated inflammation at bay. In cases of uncontrolled inflammation, there is an insufficient amount of IL-1RA to regulate the activity of interleukin-1.

References to Product

References

  • Roger Y, Schäck LM,Koroleva A, Noack S, Kurselis K, Krettek C, Chichkov B, Lenarz T, Warnecke A, Hoffmann A. Grid-like surface structures in thermoplastic polyurethane induce anti-inflammatory and anti-fibrotic processes in bone marrow-derived mesenchymal stem cells. Colloids and Surfaces B: Bioin. June 2016;
Related Products Documents