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Manufactured by BioVendor

Interleukin-10 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:IL-10, Cytokine synthesis inhibitory factor, B cell derived T cell growth factor, Mast cell growth factor III), Insulin receptor substrate (IRS)
  • Species:Human
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New RD194572200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma

Storage/Expiration

Store the complete kit at 2 8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

3.13 – 200 pg/ml

Limit of Detection

1.32 pg/ml

Intra-assay (Within-Run)

n = 8; CV = 1.9%

Inter-assay (Run-to-Run)

n = 6; CV = 4.2%

Spiking Recovery

102.9%

Dilutation Linearity

97.0%

Summary

Features

  • It is intended for research use only
  • The total assay time is less than 3.5 hours
  • The kit measures IL-10 protein in human serum and plasma (EDTA, citrate, heparin)
  • Assay format is 96 wells
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Cytokines and chemokines and related molecules

Summary

Interleukin-10 (IL-10) is a Type II cytokine and the “founding” member of a family of cytokines. IL-10 is an important anti-inflammatory and immunosuppressive cytokine. IL-10 is a homodimer with molecular mass of 37 kDa consisting of 160 amino acid monomers.

Important producers of IL-10 are T-helper type 2 (Th2) cells, subset of regulatory T cells designated Tr1, Th1 and Th17 cells, CD8+ T cells, monocytes, macrophages, certain subsets of dendritic cells (DCs), granulocytes including eosinophils and mast cells. Non-immune cells producing IL-10 include keratinocytes, epithelial cells and even tumor cells. Expression of IL-10 in unstimulated tissues is negligible and seems to require triggering by commensal or pathogenic flora.

The primary function of IL-10 is to down-regulate immune response and limit tissue damage. IL-10 can strongly inhibit secretion of IL-2/INF-γ by Th1 cells, IL-1, IL-6, IL-12, and tumor necrosis factor α in macrophages and dendritic cells in order to reduce tissue damage. IL-10 may be increased by transforming growth factor β (TGFβ), interferon α (IFNα), IFNβ and histamine.
IL-10 plays a crucial role in neuronal homeostasis and cell survival. IL-10 plays a protective role in microglial cultures after a pro-inflammatory insult and in rat pups born to dams infected with Escherichia coli. However, several studies have questioned the perception of IL-10 solely as an immunosuppressive cytokine because it can also stimulate immune responses by promoting the proliferation and cytotoxic activity of natural killer cells and CD8+ T-cells as well as the survival, proliferation, differentiation, MHC class II expression and antibody production of B-cells.

IL-10 plays a pivotal role during the chronic/latent stage of pulmonary tuberculosis. IL-10 is found in serum, plasma and bronchoalveolar lavage fluid of active tuberculosis patients. Increased levels of IL-10 secreted from macrophages have also been associated with certain types of cancer including prostate, breast, cervical and gastric tumors.
Since IL-10-mediated immune responses are quite important in maintaining intestinal homeostasis and commensal flora tolerance, it has been suggested that an aberration n IL-10 production may be involved in pathogenesis of Crohn´s disease and ulcerative colitis. Other autoimmune diseases, such as systemic lupus erythematosus, type 1 diabetes mellitus, psoriasis, and rheumatoid arthritis, have also been shown to be associated with IL-10.

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