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Manufactured by BioVendor

Interleukin-10 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:IL-10, Cytokine synthesis inhibitory factor, B cell derived T cell growth factor, Mast cell growth factor III), Insulin receptor substrate (IRS)
  • Species:Human
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Cat. No. Size Price


RD194572200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2 8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

3.13 – 200 pg/ml

Limit of Detection

1.32 pg/ml

Intra-assay (Within-Run)

n = 8; CV = 1.9%

Inter-assay (Run-to-Run)

n = 6; CV = 4.2%

Spiking Recovery

102.9%

Dilution Linearity

97.0%

Summary

Features

  • It is intended for research use only
  • The total assay time is less than 3.5 hours
  • The kit measures IL-10 protein in human serum and plasma (EDTA, citrate, heparin)
  • Assay format is 96 wells
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Cytokines and chemokines and related molecules

Summary

Interleukin-10 (IL-10) is a Type II cytokine and the “founding” member of a family of cytokines. IL-10 is an important anti-inflammatory and immunosuppressive cytokine. IL-10 is a homodimer with molecular mass of 37 kDa consisting of 160 amino acid monomers.

Important producers of IL-10 are T-helper type 2 (Th2) cells, subset of regulatory T cells designated Tr1, Th1 and Th17 cells, CD8+ T cells, monocytes, macrophages, certain subsets of dendritic cells (DCs), granulocytes including eosinophils and mast cells. Non-immune cells producing IL-10 include keratinocytes, epithelial cells and even tumor cells. Expression of IL-10 in unstimulated tissues is negligible and seems to require triggering by commensal or pathogenic flora.

The primary function of IL-10 is to down-regulate immune response and limit tissue damage. IL-10 can strongly inhibit secretion of IL-2/INF-γ by Th1 cells, IL-1, IL-6, IL-12, and tumor necrosis factor α in macrophages and dendritic cells in order to reduce tissue damage. IL-10 may be increased by transforming growth factor β (TGFβ), interferon α (IFNα), IFNβ and histamine.
IL-10 plays a crucial role in neuronal homeostasis and cell survival. IL-10 plays a protective role in microglial cultures after a pro-inflammatory insult and in rat pups born to dams infected with Escherichia coli. However, several studies have questioned the perception of IL-10 solely as an immunosuppressive cytokine because it can also stimulate immune responses by promoting the proliferation and cytotoxic activity of natural killer cells and CD8+ T-cells as well as the survival, proliferation, differentiation, MHC class II expression and antibody production of B-cells.

IL-10 plays a pivotal role during the chronic/latent stage of pulmonary tuberculosis. IL-10 is found in serum, plasma and bronchoalveolar lavage fluid of active tuberculosis patients. Increased levels of IL-10 secreted from macrophages have also been associated with certain types of cancer including prostate, breast, cervical and gastric tumors.
Since IL-10-mediated immune responses are quite important in maintaining intestinal homeostasis and commensal flora tolerance, it has been suggested that an aberration n IL-10 production may be involved in pathogenesis of Crohn´s disease and ulcerative colitis. Other autoimmune diseases, such as systemic lupus erythematosus, type 1 diabetes mellitus, psoriasis, and rheumatoid arthritis, have also been shown to be associated with IL-10.

Product References (3)

References

  • Siddiqui S, Gurung RL, Liu S, Ping Seet EC, Lim SC. Genetic Polymorphisms and Cytokine Profile of Different Ethnicities inSeptic Shock Patients and theirAssociation with Mortality. Indian J Crit Care Med. 2019 Mar;23(3):135-138. doi: 10.5005/jp-journals-10071-23136. PubMed PMID: 31097890; PubMed Central PMCID:PMC6487620. See more on PubMed
  • Trinchese G, Cavaliere G, Cimmino F, Catapano A, Carta G, Pirozzi C, Murru E, Lama A, Meli R, Bergamo P, Banni S, Mollica MP. Decreased Metabolic Flexibility in Skeletal Muscle of Rat Fed with a High-Fat Diet Is Recovered by Individual CLA Isomer Supplementation via Converging Protective Mechanisms. Cells. 2020 Mar 29;9(4):823. doi: 10.3390/cells9040823. PMID: 32235294; PMCID: PMC7226748. See more on PubMed
  • Trinchese G, Cimmino F, Cavaliere G, Rosati L, Catapano A, Sorriento D, Murru E, Bernardo L, Pagani L, Bergamo P, Scudiero R, Iaccarino G, Greco L, Banni S, Crispino M, Mollica MP. Heart Mitochondrial Metabolic Flexibility and Redox Status Are Improved by Donkey and Human Milk Intake. Antioxidants (Basel). 2021 Nov 13;10(11):1807. doi: 10.3390/antiox10111807. PMID: 34829678; PMCID: PMC8614950. See more on PubMed
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