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Manufactured by BioVendor

Interleukin-10 Rat ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:IL-10, Cytokine synthesis inhibitory factor, B cell derived T cell growth factor, Mast cell growth factor III), Insulin receptor substrate (IRS)
  • Species:Rat
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Cat. No. Size Price


RAF028R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Cell culture supernatant

Sample Requirements

50 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

15.6–1000 pg/ml

Limit of Detection

1.5 pg/ml

Intra-assay (Within-Run)

CV = 5.0%

Inter-assay (Run-to-Run)

CV = 0,1%

Dilutation Linearity

112,00%

Summary

Research topic

Cytokines and chemokines and related molecules, Animal studies

Summary

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes.
The murine IL-10 exhibits strong DNA and aminoacid sequence homology to the human IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction.

Product References (2)

References

  • Mahmoud MF, Gama Sl, Shaheen MA, El-Fayoumi HM. The effects of tramadol on hepatic ischemia/reperfusion injury in rats. Indian Journal of Pharmacology. 23-May-2016;48 (3):275-280
  • Trinchese G, Cavaliere G, Penna E, et al. Milk From Cow Fed With High Forage/Concentrate Ratio Diet: Beneficial Effect on Rat Skeletal Muscle Inflammatory State and Oxidative Stress Through Modulation of Mitochondrial Functions and AMPK Activity. Front Physiol. 2019;9:1969. Published 2019 Jan 17. doi:10.3389/fphys.2018.0196
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