Sandwich ELISA, Biotin-labelled antibody
The human IL-17A ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-17A. The human IL-17A ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Serum, Plasma-Heparin, Plasma-Citrate, Cell culture supernatant
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Limit of Detection
CV = 7.1%
CV = 9.1%
serum 107.0%, heparin plasma 50.8%, citrate plasma 75,5%, cell culture supernatant 113.6%
plasma 107.8%, cell culture supernatant 81.60%
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected.
- calibration range 1.6-100 pg/ml
- limit of detection 0.5 pg/ml
- intra-assay CV = 7.1%
- inter-assay CV = 9.1%
Cytokines and chemokines and related molecules
A new family of cytokines, Interleukin-17, has recently been defined that reveals a distinct ligand-receptor signaling system. There is high evidence for its importance in the regulation of immune responses. IL-17A was first characterised and six IL-17 family members (IL-17A-F) have subsequently been described. IL-17A, a homodimeric cytokine of about 32 kDa, is largely produced by activated memory T lymphocytes, but stimulates innate immunity and host defense. IL-17A and IL-17F both mobilize neutrophils partly through granulopoeisis and CXC chemokine induction, as well as increased survival locally. IL-17A and IL-17F production by T lymphocytes is regulated by IL-23 independent of T cell receptor activation. The T help 1 (Th1) and Th2 cell classification has until recently provided the framework for understanding CD4(+) T cell biology and the interplay between innate and adaptive immunity. Recent studies have defined a previously unknown arm of the CD4(+) T cell effector response, the Th17 lineage. This subset of T cells produces interleukin 17, which is highly proinflammatory and induces severe autoimmunity. Whereas IL-23 serves to expand previously differentiated T(H)-17 cell populations, IL-6 and transforming growth factor-beta (TGF-beta) induce the differentiation of T(H)-17 cells from naive precursors. Increasing evidence shows that IL-17 family members play an active role in inflammatory diseases, autoimmune diseases, and cancer. The IL-17 signaling system is operative in disparate tissues such as articular cartilage, bone, meniscus, brain, hematopoietic tissue, kidney, lung, skin and intestine. Thus, the evolving IL-17 family of ligands and receptors may play an important role in the homeostasis of tissues in health and disease beyond the immune system. Increased levels of IL-17 have been associated with several conditions, including airway inflammation, rheumatoid arthritis, intraperitoneal abscesses and adhesions, inflammatory bowel disease,allograft rejection, psoriasis, cancer and multiple sclerosis.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
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