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Human Interleukin-17A ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:IL-17, Interleukin-17A
  • Species:Human
Cat. No. Size Price

RAF039R 96 wells (1 kit) $721,88
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


The human IL-17A ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-17A. The human IL-17A ELISA is for research use only. Not for diagnostic or therapeutic procedures.


Serum, Plasma-Heparin, Plasma-Citrate, Cell culture supernatant

Sample Requirements

50 µl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

1.6–100 pg/ml

Limit of Detection

0.5 pg/ml

Intra-assay (Within-Run)

CV = 7.1%

Inter-assay (Run-to-Run)

CV = 9.1%

Spiking Recovery

serum 107.0%, heparin plasma 50.8%, citrate plasma 75,5%, cell culture supernatant 113.6%

Dilution Linearity

plasma 107.8%, cell culture supernatant 81.60%


The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected.



  • RUO
  • calibration range 1.6-100 pg/ml
  • limit of detection 0.5 pg/ml
  • intra-assay CV = 7.1%
  • inter-assay CV = 9.1%

Research topic

Cytokines and chemokines and related molecules


A new family of cytokines, Interleukin-17, has recently been defined that reveals a distinct ligand-receptor signaling system. There is high evidence for its importance in the regulation of immune responses. IL-17A was first characterised and six IL-17 family members (IL-17A-F) have subsequently been described. IL-17A, a homodimeric cytokine of about 32 kDa, is largely produced by activated memory T lymphocytes, but stimulates innate immunity and host defense. IL-17A and IL-17F both mobilize neutrophils partly through granulopoeisis and CXC chemokine induction, as well as increased survival locally. IL-17A and IL-17F production by T lymphocytes is regulated by IL-23 independent of T cell receptor activation. The T help 1 (Th1) and Th2 cell classification has until recently provided the framework for understanding CD4(+) T cell biology and the interplay between innate and adaptive immunity. Recent studies have defined a previously unknown arm of the CD4(+) T cell effector response, the Th17 lineage. This subset of T cells produces interleukin 17, which is highly proinflammatory and induces severe autoimmunity. Whereas IL-23 serves to expand previously differentiated T(H)-17 cell populations, IL-6 and transforming growth factor-beta (TGF-beta) induce the differentiation of T(H)-17 cells from naive precursors. Increasing evidence shows that IL-17 family members play an active role in inflammatory diseases, autoimmune diseases, and cancer. The IL-17 signaling system is operative in disparate tissues such as articular cartilage, bone, meniscus, brain, hematopoietic tissue, kidney, lung, skin and intestine. Thus, the evolving IL-17 family of ligands and receptors may play an important role in the homeostasis of tissues in health and disease beyond the immune system. Increased levels of IL-17 have been associated with several conditions, including airway inflammation, rheumatoid arthritis, intraperitoneal abscesses and adhesions, inflammatory bowel disease,allograft rejection, psoriasis, cancer and multiple sclerosis.

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