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Human Interleukin-18 ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:IL-18, Interferon-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, Iboctadekin, IGIF, IL1F4, IL-1g, MGC12320, IFN gamma inducing factor
  • Species:Human
Cat. No. Size Price

RAF143R 96 wells (1 kit) $721,88
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


The human IL-18 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-18. The human IL-18 ELISA is for research use only. Not for diagnostic or therapeutic procedures.


Serum, Plasma, Cell culture supernatant

Sample Requirements

50 µl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

78–5000 pg/ml

Limit of Detection

9.0 pg/ml

Intra-assay (Within-Run)

CV = 6.5%

Inter-assay (Run-to-Run)

CV = 8.1%

Spiking Recovery


Dilution Linearity



The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-18 positive serum. There was no crossreactivity detected, namely not with IL-18 precursor protein (pro-IL-18).



  • RUO
  • calibration range 78-5000 pg/ml
  • limit of detection 9 pg/ml
  • lyophilized controls
  • intra-assay CV = 6.5%
  • inter-assay CV = 8.1%

Research topic

Cytokines and chemokines and related molecules


Interleukin (IL) -18 is a newly discovered cytokine, structurally similar to IL-1, with profound effects on T-cell activation. Formerly called interferon (IFN) gamma inducing factor (IGIF), IL-18 is a novel cytokine that plays an important role in the T-cell-helper type 1 (Th1) response, primarily by its ability to induce IFN gamma production in T cells and natural killer (NK) cells. In terms of structure, IL-18 and IL-1 beta share primary amino acid sequences of the so-called „signature sequence“ motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL-18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). Therefore inhibitors of ICE activity may limit the biologic activity of IL-18 and may be useful as Th1 immunosuppressive agents. The activity of the mature IL-18 is closely related to that of IL-1. IL-18 induces gene expression and synthesis of tumor necrosis factor (TNF), IL-1, Fas Ligand, and several chemokines. IL-18 induces the differentiation of Th1 or Th2 cells depending upon cytokine milieu and genetic background. The activity of IL-18 is via an IL-18 receptor (IL-18R) complex. This complex is made up of a binding chain termed IL-18R, a member of the IL-1 receptor family previously identified as the IL-1 receptor – related protein (IL-1Rrp), and a signaling chain, also a member of the IL-1R family. The IL-18R complex recruits the IL-1R – activating kinase (IRAK) and TNF-R – associated factor –6 (TRAF-6) with subsequent activation of NF kappa B. Thus based on primary structure, three-dimensional structure, receptor family, signal transduction pathways and biological effects, IL-18 appears to be a new member of the IL-1 family. Similar to IL-1, IL-18 participates in both innate and acquired immunity. Constitutive IL-18 expression is detected from many different cells, including macrophages, keratinocytes, and osteoblasts. Human keratinocytes have been found to be the major producers of IL-18 in unprocessed form.

Product References (1)


  • Barrón-Cabrera E, González-Becerra K, Rosales-Chávez G, Mora-Jiménez A, Hernández-Cañaveral I, Martínez-López E. Low-grade chronic inflammation is attenuated by exercise training in obese adults through down-regulation of ASC gene in peripheral blood: a pilot study. Genes Nutr. 2020 Aug 27;15(1):15. doi: 10.1186/s12263-020-00674-0. PubMed PMID: 32854610. PubMed CentralPMCID: PMC7457251. See more on PubMed
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