Sandwich ELISA, Biotin-labelled antibody
Serum, Cell culture supernatant
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Limit of Detection
CV = 6.6%
CV = 6.8%
Cytokines and chemokines and related molecules
Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has
been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein with a native molecular weight of 45 to 60 kDa, with multiple subunits of 24 to 28 kDa under reducing conditions.