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Human Interleukin-5 ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:IL-5, T-cell replacing factor, Eosinophil differentiation factor, B cell differentiation factor I, T cell replacing factor, colony-stimulating factor
  • Species:Human
Cat. No. Size Price

RAF068R 96 wells (1 kit) $721,83
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


The human IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-5. The human IL-5 ELISA is for research use only. Not for diagnostic or therapeutic procedures.


Serum, Cell culture supernatant

Sample Requirements

50 µl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

7.8–500 pg/ml

Limit of Detection

1.5 pg/ml

Intra-assay (Within-Run)

CV = 6.6%

Inter-assay (Run-to-Run)

CV = 6.8%

Spiking Recovery


Dilution Linearity



The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-5 positive serum. There was no crossreactivity detected.



  • RUO
  • calibration range: 7.8-500 pg/ml
  • limit of detection 1.5 pg/ml
  • intra-assay CV = 6.6%
  • inter-assay CV = 6.8%

Research topic

Cytokines and chemokines and related molecules


Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has
been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein with a native molecular weight of 45 to 60 kDa, with multiple subunits of 24 to 28 kDa under reducing conditions.

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