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Manufactured by BioVendor

Interleukin-5 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:IL-5, T-cell replacing factor, Eosinophil differentiation factor, B cell differentiation factor I, T cell replacing factor, colony-stimulating factor
  • Species:Human
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Cat. No. Size Price

RAF068R 96 wells (1 kit)
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


Serum, Cell culture supernatant

Sample Requirements

50 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

7.8–500 pg/ml

Limit of Detection

1.5 pg/ml

Intra-assay (Within-Run)

CV = 6.6%

Inter-assay (Run-to-Run)

CV = 6.8%


Research topic

Cytokines and chemokines and related molecules


Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has
been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein with a native molecular weight of 45 to 60 kDa, with multiple subunits of 24 to 28 kDa under reducing conditions.

Product References (2)


  • Berni Canani R, Paparo L, Nocerino R, Cosenza L, Pezzella V, Di Costanzo M, Capasso M, Del Monaco V, D'Argenio V, Greco L, Salvatore F. Differences in DNA methylation profile of Th1 and Th2 cytokine genes are associated with tolerance acquisition in children with IgE-mediated cow's milk allergy. Clin Epigenetics. 2015 Mar 31;7(1):38. doi: 10.1186/s13148-015-0070-8. eCollection 2015. PubMed PMID: 25859290. PubMed CentralPMCID: PMC4391731. See more on PubMed
  • Ellis NB. The challenge of nursing home care. Am J Occup Ther. 1986 Jan;40(1):7-11. doi: 10.5014/ajot.40.1.7. PubMed PMID: 3080894. See more on PubMed
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