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KL-6 by Latex Agglutination Assay

  • Regulatory status:RUO
  • Type:Latex Agglutination Assay
  • Other names:Sialylated Carbohydrate Antigen, Krebs von den Lungen-6, Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227
  • Species:Human
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New KL-6(II) 1 kit
PubMed Product Details
Technical Data

Type

Latex Agglutination Assay

Applications

Serum, Plasma

Sample Requirements

2 µL

Storage/Expiration

Store all components of this kit at 2-10°C.

Calibration Range

70-10,000 U/mL

Summary

Features

  • Research Use Only.
  • This kit measures KL-6 in human serum and plasma.
  • Components of the kit are provided ready-to-use.

Research topic

Immune Response, Infection and Inflammation, Oncology, Pulmonary diseases, COVID-19

Summary

Sialylated carbohydrate antigen KL-6 found by Kohno in 1985 is one of the sialylated carbohydrate antigen located on MUC1, over 1 million molecular weight and transmembrane type glycoprotein expressed on epithelial cell line1. At the beginning of his study, he prepared monoclonal antibody by immunizing (VMRC-LCR) cell derived from human lung adenocarcinoma to mice with the aim of developing lung cancer marker. However, this antibody was specific to interstitial pneumonia and showed higher values in the active phase of interstitial pneumonia when react with patient sera, and was named KL-6 for the antigen and the antibody. In patients with interstitial pneumonia affected alveolar epithelium, KL-6 indicates remarkably higher values than in healthy people and other respiratory diseases. For this reason, KL-6 had been recognized as high diagnostic utility for interstitial pneumonia and pulmonary fibrosis, until then it was poor in specific markers for these diseases. Furthermore, in active interstitial pneumonia cases KL-6 showed significantly higher value compared to the non-active cases and vary to reflect the disease after the start of treatment. Thus, measurement of the KL-6 is used for the purpose of differentiation of pathologies characterized by fibrosis of the lungs, pathological state of interstitial pneumonia (active and inactive of differentiation) and observation of treatment.

Summary References (15)

References to KL-6

  • Arase Y, Ikeda K, Suzuki Y, Kobayashi M, Suzuki F, Akuta N, Sezaki H, Hosaka T, Yatsuji H, Kawamura Y, Kobayashi M, Kumada H. Serum KL-6 level is elevated in chronic hepatitis C patients with combination therapy of pegylated interferon and ribavirin. Intern Med. 2007;46 (15):1155-60
  • Fujiwara Y, Kiura K, Toyooka S, Hotta K, Tabata M, Takigawa N, Soh J, Tanimoto Y, Kanehiro A, Kato K, Date H, Tanimoto M. Elevated serum level of sialylated glycoprotein KL-6 predicts a poor prognosis in patients with non-small cell lung cancer treated with gefitinib. Lung Cancer. 2008 Jan;59 (1):81-7
  • Hamada H, Kohno N, Akiyama M, Hiwada K. Monitoring of serum KL-6 antigen in a patient with radiation pneumonia. Chest. 1992 Mar;101 (3):858-60
  • Kida Y, Maeshima E, Furukawa K, Ichikawa T, Goda M, Ichinose M. A case of polymyositis with a significantly high level of KL-6 associated with pancreatic cancer. Mod Rheumatol. 2007;17 (3):262-4
  • Kikuchi N, Ishii Y, Ohtsuka M. Serum KL-6 levels and acute lung inflammation. Intern Med. 2007;46 (6):335; author reply 337
  • Kobayashi J, Kitamura S. KL-6: a serum marker for interstitial pneumonia. Chest. 1995 Aug;108 (2):311-5
  • Kobayashi J, Kitamura S. Serum KL-6 for the evaluation of active pneumonitis in pulmonary sarcoidosis. Chest. 1996 May;109 (5):1276-82
  • Kohno N, Akiyama M, Kyoizumi S, Hakoda M, Kobuke K, Yamakido M. Detection of soluble tumor-associated antigens in sera and effusions using novel monoclonal antibodies, KL-3 and KL-6, against lung adenocarcinoma. Jpn J Clin Oncol. 1988 Sep;18 (3):203-16
  • Kohno N, Awaya Y, Oyama T, Yamakido M, Akiyama M, Inoue Y, Yokoyama A, Hamada H, Fujioka S, Hiwada K. KL-6, a mucin-like glycoprotein, in bronchoalveolar lavage fluid from patients with interstitial lung disease. Am Rev Respir Dis. 1993 Sep;148 (3):637-42
  • Kohno N, Hamada H, Fujioka S, Hiwada K, Yamakido M, Akiyama M. Circulating antigen KL-6 and lactate dehydrogenase for monitoring irradiated patients with lung cancer. Chest. 1992 Jul;102 (1):117-22
  • Kohno N, Kyoizumi S, Awaya Y, Fukuhara H, Yamakido M, Akiyama M. New serum indicator of interstitial pneumonitis activity. Sialylated carbohydrate antigen KL-6. Chest. 1989 Jul;96 (1):68-73
  • Kohno N, Kyoizumi S, Tanabe M, Oyama T, Vossler M, Yamakido M, Akiyama M. Detection of a circulating tumor-associated antigen with a murine monoclonal antibody, LISA 101, selected by reversed indirect enzyme-linked immunosorbent assay. Cancer Res. 1989 Jun 15;49 (12):3412-9
  • Kunitake R, Kuwano K, Yoshida K, Maeyama T, Kawasaki M, Hagimoto N, Hara N. KL-6, surfactant protein A and D in bronchoalveolar lavage fluid from patients with pulmonary sarcoidosis. Respiration. 2001;68 (5):488-95
  • Ohtsuki Y, Nakanishi N, Fujita J, Yoshinouchi T, Kobayashi M, Ueda N, Lee GH, Furihata M. Immunohistochemical distribution of SP-D, compared with that of SP-A and KL-6, in interstitial pneumonias. Med Mol Morphol. 2007 Sep;40 (3):163-7
  • Tang W, Guo Q, Qu X, Inagaki Y, Seyama Y, Midorikawa Y, Gai R, Kokudo N, Sugawara Y, Nakata M, Makuuchi M. KL-6 mucin is a useful immunohistochemical marker for cholangiocarcinoma. Oncol Rep. 2007 Apr;17 (4):737-41
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