Select country change
Shopping cart (0 , 0,00 ) Menu Search
Manufactured by BioVendor

Leptin Mouse/Rat ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Obesity factor, Obese protein, LEP, OB, OBS
  • Species:Mouse, Rat
Please select your region to see available products and prices.
Cat. No. Size Price


RD291001200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

7 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

100–4000 pg/ml

Limit of Detection

Analytical Limit of Detection is calculated from the real leptin values in wells and is 30 pg/ml for mouse leptin and 50 pg/ml for rat leptin

Intra-assay (Within-Run)

n = 8; CV = 2.2%

Inter-assay (Run-to-Run)

n = 8; CV = 3.4%

Spiking Recovery

94,50%

Dilutation Linearity

96,70%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • monkey Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • sheep Not tested
  • chicken Not tested
  • mouse Yes
  • rat Yes
  • human Yes (recommended dilution 1:3)
Summary

Features

  • The total assay time is less than four hours.
  • The kit measures total serum leptin.
  • Quality controls are mouse and rat serum based. No human sera are used.

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Reproduction, Animal studies

Summary

Leptin is a protein hormone with important effects in metabolism and regulating body weight. It is a single-chain 16 kDa protein consisting of 146 amino acid residues and encoded by the obese (ob) gene.
Leptin is expressed predominantly by adipocytes, small amounts of leptin are also secreted by cells in the epithelium of stomach and in the placenta. Leptin´s effect on body weight is mediated through effects on hypothalamic centers, where leptin receptors are highly expressed. Leptin has a dual action, it decreases the appetite and increases energy consumption.

A mutations in the ob gene of leptin or in the gene of leptin receptor causes hyperphagia, reduced energy expenditure, and severe obesity in the ob/ob mice.
Ob gene knockout mice are also characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperglycaemia, hyperinsulinemia and insulin resistance.
When ob/ob mice are treated with injections of leptin, they lose their excess fat and return to normal body weight.
Studies have shown that leptin appears to be a significant regulator of reproductive function. In addition, leptin is involved in bone metabolism and plays a significant role as an immunomodulator.

References to Product

References

  • Arimochi H, Sasaki Y, Kitamura A, Yasutomo K. Differentiation of preadipocytes and mature adipocytes requires PSMB8. Sci Rep. 2016;6:26791
  • Bartels ED, Nielsen JM, Hellgren LI, Ploug T, Nielsen LB. Cardiac expression of microsomal triglyceride transfer protein is increased in obesity and serves to attenuate cardiac triglyceride accumulation. PLoS One. 2009;4 (4):e5300
  • Belemets N, Kobyliak N, Virchenko O, Falalyeyeva T, Olena T, Bodnar P, SavchukO, Galenova T, Caprnda M, Rodrigo L, Skladany L, Delev D, Opatrilova R, Kruzliak P, Beregova T, Ostapchenko L. Effects of polyphenol compounds melanin onNAFLD/NASH prevention. Biomed Pharmacother. 2017 Apr;88:267-276. doi:10.1016/j.biopha.2017.01.028
  • Bol VV, Delattre AI, Reusens B, Raes M, Remacle C. Forced catch-up growth after fetal protein restriction alters the adipose tissue gene expression program leading to obesity in adult mice. Am J Physiol Regul Integr Comp. 2009 Aug;297 (2):R291-9
  • Bolze F, Bast A, Mocek S, Morath V, Yuan D, Rink N, Schlapschy M, Zimmermann A, Heikenwalder M, Skerra A, Klingenspor M. Treatment of diet-induced lipodystrophic C57BL/6J mice with long-acting PASylated leptin normalises insulin sensitivity and hepatic steatosis by promoting lipid utilisation. Diabetologia. 2016 Jun 7;
  • De Jong A, Carreira S, Na N, Carillion A, Jiang C, Beuvin M, Lacorte JM, Bonnefont-Rousselot D, Riou B, Coirault C. Diaphragmatic function is enhanced in fatty and diabetic fatty rats. PLoS One. 2017 Mar 22;12(3):e0174043. doi:10.1371/journal.pone.0174043
  • Desmoulins L, Chrétien C, Paccoud R, et al. Mitochondrial Dynamin-Related Protein 1 (DRP1) translocation in response to cerebral glucose is impaired in a rat model of early alteration in hypothalamic glucose sensing. Mol Metab. 2018;20:166-177.
  • Dupas J, Feray A, Guernec A, et al. Effect of personalized moderate exercise training on Wistar rats fed with a fructose enriched water. Nutr Metab (Lond). 2018;15:69. Published 2018 Oct 3. doi:10.1186/s12986-018-0307-6
  • Giri S, Rattan R, Hag E, Khan M, Yasmin R, Won JS, Key L, Singh AK, Singh I. AICAR inhibits adipocyte differentiation in 3T3L1 and restores metabolic alterations in diet-induced obesity mice model. Nutr Metab (Lond) . Aug 10;3:31 (2006)
  • Gout J, Sarafian D, Tirard J, Blondet A, Vigier M, Rajas F, Mithieux G, Begeot M, Naville D. Leptin infusion and obesity in mouse cause alterations in the hypothalamic melanocortin system. Obesity (Silver Spring). 2008 Aug;16 (8):1763-9
  • HORI M, MUTOH M, ISHIGAMORI R, IMAI T, TAKAHASHI M. Activated Ductal Proliferation Induced by N-Nitrosobis (2-oxopropyl)amine in Fat-infiltrated Pancreas of KK-Ay Mice. In Vivo. 2018;32(3):409–505. Published 2018 May 3. doi:10.21873/invivo.11267
  • Kenny S, Gamble J, Lyons S, Vlatkovic N, Dimaline R, Varro A, Dockray GJ. Gastric expression of plasminogen activator inhibitor (PAI)-1 is associated with hyperphagia and obesity in mice. Endocrinology. 2013 Feb;154 (2):718-26
  • Kirk SL, Samuelsson AM, Argenton M, Dhonye H, Kalamatianos T, Poston L, Taylor PD, Coen CW. Maternal obesity induced by diet in rats permanently influences central processes regulating food intake in offspring. PLoS One. 2009;4 (6):e5870
  • Lambert K, Hokayem M, Thomas C, et al. Combination of nutritional polyphenols supplementation with exercise training counteracts insulin resistance and improves endurance in high-fat diet-induced obese rats. Sci Rep. 2018;8(1):2885. Published 2018 Feb 13. doi:10.1038/s41598-018-21287-z
  • Lambert K, Hokayem M, Thomas C, et al. No Additive Effects of Polyphenol Supplementation and Exercise Training on White Adiposity Determinants of High-Fat Diet-Induced Obese Insulin-Resistant Rats. Oxid Med Cell Longev. 2018;2018:7406946. Published 2018 Apr 15. doi:10.1155/2018/7406946
  • Liao YH, Chen CY, Chen CN, Wu CY, Tsai SC. An Amino Acids Mixture Attenuates Glycemic Impairment but not Affects Adiposity Development in Rats Fed with AGEs-containing Diet. Int J Med Sci. 2018;15(2):176-187. Published 2018 Jan 1. doi:10.7150/ijms.22008
  • Marín-Royo G, Ortega-Hernández A, Martínez-Martínez E, et al. The Impact of Cardiac Lipotoxicity on Cardiac Function and Mirnas Signature in Obese and Non-Obese Rats with Myocardial Infarction. Sci Rep. 2019;9(1):444. Published 2019 Jan 24. doi:10.1038/s41598-018-36914-y
  • Putakala M, Gujjala S, Nukala S, Desireddy S. Beneficial Effects of Phyllanthus amarus Against High Fructose Diet Induced Insulin Resistance and Hepatic Oxidative Stress in Male Wistar Rats. Appl Biochem Biotechnol. 2017 Mar 28. doi: 10.1007/s12010-017-2461-0
  • Ramirez-Lopez MT, Arco R, Decara J, Vazquez M, Noemi Blanco R, Alen F, Suarez J, Gomez de Heras R, Rodriguez de Fonseca F. Exposure to a Highly Caloric Palatable Diet during the Perinatal Period Affects the Expression of the Endogenous Cannabinoid System in the Brain, Liver and Adipose Tissue of Adult Rat Offspring. PLoS One. 2016;11 (11):e0165432
  • Rodriguez L, Panadero MI, Rodrigo S, Roglans N, Otero P, Alvarez-Millan JJ, Laguna JC, Bocos C. Liquid fructose in pregnancy exacerbates fructose-induced dyslipidemia in adult female offspring. J Nutr Biochem. 2016 Jun;32:115-22
  • Rohrbach S, Aurich AC, Li L, Niemann B. Age-associated loss in adiponectin-activation by caloric restriction: lack of compensation by enhanced inducibility of adiponectin paralogs CTRP2 and CTRP7. Mol Cell Endocrinol. 2007 Oct 15;277 (1-2):26-34
  • Sharma V, Smolin J, Nayak J, et al. Mannose Alters Gut Microbiome, Prevents Diet-Induced Obesity, and Improves Host Metabolism. Cell Rep. 2018;24(12):3087-3098.
  • Stejskal D, Karpisek M, Hanulova Z, Svestak M. Chemerin is an independent marker of the metabolic syndrome in a Caucasian population--a pilot study. Biomed Pap Med Fac Univ Palack. 2008 Dec;152 (2):217-21
  • Tekin S, Erden Y, Ozyalin F, Cigremis Y, Colak C, Sandal S. The effects of intracerebroventricular infusion of irisin on feeding behaviour in rats. Neurosci Lett. 2017 Apr 3;645:25-32. doi: 10.1016/j.neulet.2017.02.066
  • Tiao MM, Lin YJ, Yu HR, et al. Resveratrol ameliorates maternal and post-weaning high-fat diet-induced nonalcoholic fatty liver disease via renin-angiotensin system. Lipids Health Dis. 2018;17(1):178. Published 2018 Jul 28. doi:10.1186/s12944-018-0824-3
  • Tinkov AA, Gatiatulina ER, Popova EV, Polyakova VS, Skalnaya AA, Agletdinov EF, Nikonorov AA, Skalny AV. Early High-Fat Feeding Induces Alteration of Trace Element Content in Tissues of Juvenile Male Wistar Rats. Biol Trace Elem Res. 2016 Jun 16;
  • Tolman JR, Lephart ED, Setchell KD, Eggett DL, Christensen MJ. Timing of supplementation of selenium and isoflavones determines prostate cancer risk factor reduction in rats. Nutr Metab (Lond). 2008;5:31
  • Tsai CC, Tiao MM, Sheen JM, et al. Obesity programmed by prenatal dexamethasone and postnatal high-fat diet leads to distinct alterations in nutrition sensory signals and circadian-clock genes in visceral adipose tissue. Lipids Health Dis. 2019;18(1):19. Published 2019 Jan 18. doi:10.1186/s12944-019-0963-1
  • Ziomber A, Rokita E, Kaszuba-Zwoinska J, Romańska I, Michaluk J, Antkiewicz-Michaluk L. Repeated Transcranial Direct Current Stimulation Induces Behavioral, Metabolic and Neurochemical Effects in Rats on High-Calorie Diet. Front Behav Neurosci. 2018;11:262. Published 2018 Jan 15. doi:10.3389/fnbeh.2017.00262
References to Summary

References to Leptin

  • Auwerx J, Staels B. Leptin (Review article). The Lancet . 13, 737 (1998);
  • Blum WF, Englaro P, Hanitsch S, Juul A, Hertel NT, Muller J, Skakkebaek NE, Heiman ML, Birkett M, Attanasio AM, Kiess W, Rascher W. Plasma leptin levels in healthy children and adolescents: dependence on body mass index, body fat mass, gender, pubertal stage, and testosterone. J Clin Endocrinol Metab. 1997 Sep;82 (9):2904-10
  • Chehab FF, Mounzih K, Lu R, Lim ME. Early onset of reproductive function in normal female mice treated with leptin. Science. 1997 Jan 3;275 (5296):88-90
  • Clement K, Vaisse C, Lahlou N, Cabrol S, Pelloux V, Cassuto D, Gourmelen M, Dina C, Chambaz J, Lacorte JM, Basdevant A, Bougneres P, Lebouc Y, Froguel P, Guy-Grand B. A mutation in the human leptin receptor gene causes obesity and pituitary dysfunction. Nature. 1998 Mar 26;392 (6674):398-401
  • Cohen B, Novick D, Rubinstein M. Modulation of insulin activities by leptin. Science. 1996 Nov 15;274 (5290):1185-8
  • Considine RV, Sinha MK, Heiman ML, Kriauciunas A, Stephens TW, Nyce MR, Ohannesian JP, Marco CC, McKee LJ, Bauer TL, et al. Serum immunoreactive-leptin concentrations in normal-weight and obese humans. N Engl J Med. 1996 Feb 1;334 (5):292-5
  • Friedman JM, Halaas JL. Leptin and the regulation of body weight in mammals. Nature. 1998 Oct 22;395 (6704):763-70
  • Halaas JL, Gajiwala KS, Maffei M, Cohen SL, Chait BT, Rabinowitz D, Lallone RL, Burley SK, Friedman JM. Weight-reducing effects of the plasma protein encoded by the obese gene. Science. 1995 Jul 28;269 (5223):543-6
  • Harigaya A, Nagashima K, Nako Y, Morikawa A. Relationship between concentration of serum leptin and fetal growth. J Clin Endocrinol Metab. 1997 Oct;82 (10):3281-4
  • Lonnqvist F, Arner P, Nordfors L, Schalling M. Overexpression of the obese (ob) gene in adipose tissue of human obese subjects. Nat Med. 1995 Sep;1 (9):950-3
  • Maffei M, Halaas J, Ravussin E, Pratley RE, Lee GH, Zhang Y, Fei H, Kim S, Lallone R, Ranganathan S, et al. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nat Med. 1995 Nov;1 (11):1155-61
  • Montague CT, Farooqi IS, Whitehead JP, Soos MA, Rau H, Wareham NJ, Sewter CP, Digby JE, Mohammed SN, Hurst JA, Cheetham CH, Earley AR, Barnett AH, Prins JB, O'Rahilly S. Congenital leptin deficiency is associated with severe early-onset obesity in humans. Nature. 1997 Jun 26;387 (6636):903-8
  • Pelleymounter MA, Cullen MJ, Baker MB, Hecht R, Winters D, Boone T, Collins F. Effects of the obese gene product on body weight regulation in ob/ob mice. Science. 1995 Jul 28;269 (5223):540-3
  • Ricci MR, Lee MJ, Russell CD, Wang Y, Sullivan S, Schneider SH, Brolin RE, Fried SK. Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms. Am J Physiol Endocrinol Metab. 2005 Apr;288 (4):E798-804
  • Schubring C, Prohaska F, Prohaska A, Englaro P, Blum W, Siebler T, Kratzsch J, Kiess W. Leptin concentrations in maternal serum and amniotic fluid during the second trimenon: differential relation to fetal gender and maternal morphometry. Eur J Obstet Gynecol Reprod Bi. 1999 Oct;86 (2):151-7
  • Spiegelman BM, Flier JS. Adipogenesis and obesity: rounding out the big picture. Cell. 1996 Nov 1;87 (3):377-89
  • Tartaglia LA. The leptin receptor. J Biol Chem. 1997 Mar 7;272 (10):6093-6
  • Tritos NA, Mantzoros CS. Leptin: its role in obesity and beyond. Diabetologia. 1997 Dec;40 (12):1371-9
  • Zhang F, Basinski MB, Beals JM, Briggs SL, Churgay LM, Clawson DK, DiMarchi RD, Furman TC, Hale JE, Hsiung HM, Schoner BE, Smith DP, Zhang XY, Wery JP, Schevitz RW. Crystal structure of the obese protein leptin-E100. Nature. 1997 May 8;387 (6629):206-9
  • Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman JM. Positional cloning of the mouse obese gene and its human homologue. Nature. 1994 Dec 1;372 (6505):425-32
Related Products Documents