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Leptin Receptor Human, Mouse Monoclonal Antibody, Clone: LPR-01

  • Regulatory status:RUO
  • Type:Monoclonal Antibody
  • Species:Human
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Cat. No. Size Price


RD182002110-01 0.1 mg
PubMed Product Details
Technical Data

Type

Monoclonal Antibody

Applications

Western blotting, ELISA, Immunohistochemistry

Antibodies Applications

Source of Antigen

NSO mouse myeloma cell line

Hosts

Mouse

Isotype

IgG1

Clone

LPR-01

Preparation

The antibody is a mouse monoclonal antibody against recombinant Human Leptin Receptor. The Human Leptin Receptor is a recombinant protein produced in mouse myeloma cell line. DNA sequence including the extracellular domain of Leptin Receptor (amino acid residues 1 839) fused to the Fc region of human IgG (with IIEGR added at the amino terminus and 6 histidine residues added at the carboxy terminus).

Species Reactivity

Human. Does not react with mouse. Not yet tested in other species.

Purification Method

Affinity chromatography on a column with immobilized protein G.

Antibody Content

0.1 mg (determined by BCA method, BSA was used as a standard)

Formulation

The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Quality Control Test

SDS PAGE – to determine purity of the antibody.

Note

This product is for research use only.

Summary

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Reproduction

Summary

Leptin receptor (OB R) was identified as a leptin binding protein (Leptin, the product of the ob gene, is a single chain 16 kDa protein consisting of 146 amino acid residues.) OB R was found to be a member of the class I cytokine receptor family with a large extracellular domain comprising 816 amino acid residues. Leptin receptor exists in multiple forms with a common extracellular domain and a variable length cytoplasmatic portion. Alternate splicing from a single gene derives the six isoforms of the Leptin receptor. The soluble form of the Leptin receptor, OB R contains no intracellular motifs or transmembrane residues, thus it consists entirely of the extracellular ligand binding domain of the receptor. Long forms of OB-R transcripts were reported to be expressed predominantly in regions of the hypothalamus which provides evidence that Leptin receptor is important in body weight regulation. Expression of short forms of OB-R transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney, and primitive hematopoietic cell populations. Leptin receptor may act as a negative regulator of Leptin activity and it may maintain a pool of available bioactive Leptin by binding and delaying its clearance from circulation. Soluble Leptin receptor levels are indirectly proportional to adiposity and are increased in females versus males. Leptin receptor levels are highest in infants, decrease into adolescence, and remain relatively stable throughout adulthood. Soluble Leptin receptor is also found upregulated in patients with chronic heart failure, end-stage renal disease and anorexia.

References to Summary

References to Leptin Receptor

  • Lahlou N. et al.: Soluble leptin receptor in serum of subjects with complete resistance to leptin: Diabetes; 19:1347–1352 (2000)
  • Auwerx J. and Staels B.: Leptin (Review article). The Lancet; 13:737 (1998)
  • Chen H. et al.: Evidence that the diabetes gene encodes the leptin receptor: identification of a mutation in the leptin receptor gene in db/db mice. Cell; 84:491–495 (1996)
  • Ciofii J. A. et al.: Novel B219/OB receptor isoforms: possible role of leptin in hematopoiesis and reproduction. Nature Med.; 2:585–589 (1996)
  • Houseknecht K. L. and Portocarrero C. P.: Leptin and its receptors: Regulators of whole body energy homeostasis. Domestic animal endocrinology; 15 (6):457–475 (1998)
  • Lee G.H. et al.: Abnormal splicing of the leptin receptor in diabetic mice. Nature; 379:632–635 (1996)
  • Tartaglia L. A. et al.: Identification and Expresion Cloning of a Leptin Receptor, OB-R. Cell; 83:1263–1271 (1995)
  • Tartaglia L. A.: The leptin receptor. J. Biol. Chem.; 272:6093–6096 (1997)
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