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LH ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA
  • Other names:Luteinizing hormone, Lutropin, Lutrophin
  • Species:Human
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New DKO009 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA

Applications

Serum, Plasma

Sample Requirements

20 µl/well

Shipping

Shipped on ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

0 - 5 - 25 - 50 - 100 - 200 mIU/mL

Limit of Detection

0.22 mIU/mL

Intra-assay (Within-Run)

n=20
CV ≤ 9.2%

Inter-assay (Run-to-Run)

n=10
CV ≤ 10.0%

Spiking Recovery

93.2 - 101.2%

Dilution Linearity

91.7 - 106.5%

Summary

Features

European Union: for in vitro diagnostic use

Rest of the world: for research use only!

The total assay time is less than 1.5 hours

Quantitative determination of LH concentration in human serum or plasma

Assay format is 96 wells

Quality Control is ready to use

Calibartors ready to use

For routine analysis

Research topic

Reproduction

Summary

Luteinizing hormone (LH) is a glycoprotein consisting of two subunits with a molecular mass of 30,000 daltons. The α-subunit is similar to other pituitary hormones [follicle stimulating hormone (FSH), thyroid stimulating hormone (TSH) and chorionic gonadotropin (HCG)] while the β-subunit is unique. The β-subunit confers the biological activity to the molecule. The α-subunit consists of 89 amino acid residues while the β-subunit contains 129 amino acids. The carbohydrate content is between 15% and 30%. The clinical usefulness of the measurement of luteinizing hormone (LH) in ascertaining the homeostasis of fertility regulation via the hypothalamic - pituitary - gonadal axis has been well established (1,2). In addition, the advent of in vitro fertilization (IVF) technology to overcome infertility associated problems has provided the impetus for rapid improvement in LH assay methodology from the technically demanding bioassay (3) to the procedurally simple and rapid immunoenzymometric assays.

Summary References (22)

References to LH

  • Bardin CW, Paulsen CA. "The Testes" in Textbook of Endocrinology, (ed.) R.H. Williams M.D., W.B. Saunders Co. 1981;
  • Boyar R, Finkelstein J, Roffwarg H, Kapen S, Weitzman E, Hellman L. Synchronization of augmented luteinizing hormone secretion with sleep during puberty. N Engl J Med. 1972 Sep 21;287 (12):582-6
  • Cohen KL. Metabolic, endocrine, and drug-induced interference with pituitary function tests: a review. Metabolism. 1977 Oct;26 (10):1165-77
  • Cumming DC, Reid RL, Quigley ME, Rebar RW, Yen SS. Evidence for decreased endogenous dopamine and opioid inhibitory influences on LH secretion in polycystic ovary syndrome. Clin Endocrinol (Oxf). 1984 Jun;20 (6):643-8
  • Cumming DC, Vickovic MM, Wall SR, Fluker MR. Defects in pulsatile LH release in normally menstruating runners. J Clin Endocrinol Metab. 1985 Apr;60 (4):810-2
  • Engvall E. Enzyme immunoassay ELISA and EMIT. Methods Enzymol. 1980;70 (A):419-39
  • Ferin M, Van Vugt D, Wardlaw S. The hypothalamic control of the menstrual cycle and the role of endogenous opioid peptides. Recent Prog Horm Res. 1984;40:441-85
  • Harris GW, Naftolin F. The hypothalamus and control of ovulation. Br Med Bull. 1970 Jan;26 (1):3-9
  • Hoff JD, Quigley ME, Yen SS. Hormonal dynamics at midcycle: a reevaluation. J Clin Endocrinol Metab. 1983 Oct;57 (4):792-6
  • Hoff JD, Quigley ME, Yen SS. Hormonal dynamics at midcycle: a reevaluation. J Clin Endocrinol Metab. 1983 Oct;57 (4):792-6
  • Hosseinian AH, Kim MH. Predetermination of ovulation timing by luteinizing hormone assay. Fertil Steril. 1976 Apr;27 (4):369-74
  • Jeffcoate SL. The control of testicular function in the adult. Clin Endocrinol Metab. 1975 Nov;4 (3):521-43
  • Knobil E. The neuroendocrine control of the menstrual cycle. Recent Prog Horm Res. 1980;36:53-88
  • Krieger DT. Placenta as a source of 'brain' and 'pituitary' hormones. Biol Reprod. 1982 Feb;26 (1):55-71
  • Marshall JC. Clinics in endocrinology and metabolism. Investigative procedures. Clin Endocrinol Metab. 1975 Nov;4 (3):545-67
  • Pierce JG, Parsons TF. Glycoprotein hormones: structure and function. Annu Rev Biochem. 1981;50:465-95
  • Rebar R, Judd HL, Yen SS, Rakoff J, Vandenberg G, Naftolin F. Characterization of the inappropriate gonadotropin secretion in polycystic ovary syndrome. J Clin Invest. 1976 May;57 (5):1320-9
  • Ross GT, VandeWeile RL, Frantz AG. Chapter 7 in "The Ovaries and the Breasts" in "Textbook of Endocrinology" (R. H. Williams, Ed.), W.B. Saunders Co. 1981;
  • Shome B, Parlow AF. Human follicle stimulating hormone (hFSH): first proposal for the amino acid sequence of the alpha-subunit (hFSHa) and first demonstration of its identity with the alpha-subunit of human luteinizing hormone (hLHa). J Clin Endocrinol Metab. 1974 Jul;39 (1):199-202
  • Simpson ER, MacDonald PC. Endocrine physiology of the placenta. Annu Rev Physiol. 1981;43:163-88
  • Uotila M, Ruoslahti E, Engvall E. Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein. J Immunol Methods. 1981;42 (1):11-5
  • Whitley RJ, Keutmann HT, Ryan RJ. Studies on the isolation and chemical-physical properties of porcine follicle-stimulating hormone. Endocrinology. 1978 Jun;102 (6):1874-86
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