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LH (S-type) Rat ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Luteinizing hormone, Lutropin, Lutrophin
  • Species:Rat
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Cat. No. Size Price

630-23929 96 wells (1 kit)
PubMed Product Details
Technical Data

Cat # changed from RSHAKRLH-010SR to 630-23929


Sandwich ELISA, Biotin-labelled antibody


Serum, Plasma

Sample Requirements

10 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.313–10 ng/ml

Limit of Detection

31.3 pg/ml


Research topic

Reproduction, Animal studies


Luteinizing hormone (LH) is produced in both men and women from the anterior pituitary gland in response to luteinizing hormone-releasing hormone (LH-RH or Gn-RH), which is released by the hypothalamus. LH, also called interstitial cell-stimulating hormone (ICSH) in men, is a glycoprotein with a molecular weight of approximately 30.000 daltons. It is composed of two non covalently associated dissimilar amino acid chains, alpha and beta. The alpha chain is similar to that found in human thyroid-stimulating hormone (TSH), follicle stimulating hormone (FSH), and human chorionic gonadotropin (hCG). The difference between these hormones lie in the amino acid composition of their beta subunits, which account for their immunological differentiation. The basal secretion of LH in men is episodic and has the primary function of stimulating the interstitial cells (Leydig cells) to produce testosterone. The variation in LH concentrations in women is subject to the complex ovulatory cycle of healthy menstruating women, and depends upon a sequence of hormonal events along the gonado-hypothalamic-pituitary axis. The decrease in progesterone and estradiol levels from the preceeding ovulation initiates each menstrual cycle. As a result of the decrease in hormone levels, the hypothalamus increases the secretion of gonadotropin-releasing factors (GnRF), which in turn stimulates the pituitary to increase FSH production and secretion. The rising FSH levels stimulate several follicles during the follicular phase, one of these will mature to contain the egg. As the follicle develops, estradiol is secreted, slowly at first, but by day 12 or 13 of a normal cycle increasing rapidly. LH is released as a result of this rapid estradiol rise because of direct stimulation of the pituitary and increasing GnRF and FSH levels. These events constitute the preovulatory phase. Ovulation occurs approximately 12 to 18 hours after the LH reaches a maximum level. After the egg is released, corpus luteum is formed which secretes progesterone and estrogen – two feedback regulators of LH. The luteal phase rapidly follows this ovulatory phase, and is characterized by high progesterone levels, a second estradiol increase, and low LH and FSH levels. Low LH and FSH levels are the result of the negative feedback effects of estradiol and progesterone on the hypotalamic-pituitary axis. After conception, the developing embryo produces hCG, which causes the corpus luteum to continue producing progesterone and estradiol. The corpus luteum regresses if pregnancy does not occur, and the corresponding drop in progesterone and estradiol levels results in menstruation. The hypothalamus initiates the menstrual cycle again as a result of these low hormone levels. Patients suffering from hypogonadism show increased concentrations of serum LH. A decrease in steroid hormone production in females is a result of immature ovaries, primary ovarian failure, polycystic ovary disease, or menopause; in these cases, LH secretion is not regulated. A similar loss of regulatory hormones occurs in males when the testes develop abnormally or anorchia exists. High concentrations of LH may also be found in primary testicular failure and Klinefelter syndrome, although LH levels will not necessarily be elevated if the secretion of androgens continues. Increased concentrations of LH are also present during renal failure, cirrhosis, hyperthyroidism, and severe starvation. A lack of secretion by the anterior pituitary may cause lower LH levels. As may be expected, low levels may result in infertility in both males and females. Low levels of LH may also be due to the decreased secretion of GnRH by the hypothalamus, although the same effect may be seen by a failure of the anterior pituitary to respond to GnRH stimulation. Low LH values may therefore indicate some dysfunction of the pituitary or hypothalamus, but the actual source of the problem must be confirmed by other tests. In the differential diagnosis of h

Product References (3)


  • Awodele O, Badru WA, Busari AA, Kale OE, Ajayi TB, Udeh RO, Emeka PM. Toxicological evaluation of therapeutic and supra-therapeutic doses of Cellgevity® on reproductive function and biochemical indices in Wistar rats. BMC Pharmacol Toxicol. 2018 Oct 25;19(1):68. doi: 10.1186/s40360-018-0253-y. PubMed PMID: 30359320. PubMed CentralPMCID: PMC6202817. See more on PubMed
  • Awodele O, Kale OE, Odewabi AO, Ekor M, Salau BA, Adefule-Ositelu AO. Safety evaluation of Bon-santé cleanser(®) polyherbal in male Wistar rats: Further investigations on androgenic and toxicological profile. J Tradit Complement Med. 2017 Jun 20;8(1):212-219. doi: 10.1016/j.jtcme.2017.06.002. eCollection 2018 Jan. PubMed PMID: 29322011. PubMed CentralPMCID: PMC5756022. See more on PubMed
  • Kobyliak NM, Falalyeyeva TM, Kuryk OG, Beregova TV, Bodnar PM, Zholobak NM, Shcherbakov OB, Bubnov RV, Spivak MY. Antioxidative effects of cerium dioxide nanoparticles ameliorate age-related male infertility: optimistic results in rats and the review of clinical clues for integrative concept of men health and fertility. EPMA J. 2015 Jun 10;6(1):12. doi: 10.1186/s13167-015-0034-2. eCollection 2015. PubMed PMID: 26097523. PubMed CentralPMCID: PMC4475301. See more on PubMed
Summary References (22)

References to LH

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  • Ross GT, VandeWeile RL, Frantz AG. Chapter 7 in "The Ovaries and the Breasts" in "Textbook of Endocrinology" (R. H. Williams, Ed.), W.B. Saunders Co. 1981;
  • Shome B, Parlow AF. Human follicle stimulating hormone (hFSH): first proposal for the amino acid sequence of the alpha-subunit (hFSHa) and first demonstration of its identity with the alpha-subunit of human luteinizing hormone (hLHa). J Clin Endocrinol Metab. 1974 Jul;39 (1):199-202
  • Simpson ER, MacDonald PC. Endocrine physiology of the placenta. Annu Rev Physiol. 1981;43:163-88
  • Uotila M, Ruoslahti E, Engvall E. Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein. J Immunol Methods. 1981;42 (1):11-5
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