Select country change
Shopping cart (0 , 0,00 ) Menu Search
Manufactured by BioVendor

Lipocalin-2/NGAL Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:24p3, MSFI, LCN2
  • Species:Human
Please select your region to see available products and prices.
Cat. No. Size Price


RD191102200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Notice

For processing the stool samples we recommend using BioVendor Extraction Buffer. The reagent is not included and can be ordered separately (Cat. No.: C005821, 100 ml).

Please find the protocol for preparation and analysis of stool extracts in Documents.

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Urine, Stool

Sample Requirements

Serum and plasma: 10 µl/well
For processing the stool samples we recommend using BioVendor Extraction Buffer. The reagent is not included and can be ordered separately (Cat. No.: C005821, 100 ml).

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.3–10 ng/ml

Limit of Detection

0.02 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 7.7%

Inter-assay (Run-to-Run)

n = 7; CV = 9.8%

Spiking Recovery

98,00%

Dilutation Linearity

104,50%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • monkey Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • human Yes
Summary

Features

  • It is intended for research use only
  • The total assay time is less than 3.5 hours.
  • The kit measures Lipocalin-2 in serum, plasma (EDTA, citrate, heparin), urine and stool extract
  • For protocol for preparation of stool extracts and other details, please contact us at info@biovendor.com
  • Assay format - 96 wells
  • Quality controls are human serum based. No animal sera are used.
  • Standard is recombinant protein based.
  • Components of kit are provided ready to use, concentrated or lyophilized.

Research topic

Energy metabolism and body weight regulation, Renal disease

Summary

Lipocalin-2 (LCN2) is a 25 kDa secretory glycoprotein, also called NGAL (neutrophil gelatinase-associated lipocalin); NL (neutrophil lipocalin); p25; oncogen 24p3 and 25 kDa alpha-2-microglobulin-related subunit of MMP-9 (LCN2 forms a covalently linked, disulfide-bridged heterodimer with the 92 kDa type V collagenase (MMP-9)). LCN2 is predominantly expressed in adipose tissue and liver. It belongs to the lipocalin superfamily that consists of over 20 small secretory proteins. Lipocalin folds consist of 8 antiparallel ß-sheets that surround a hydrophobic pocket. A common feature of this protein family, following from its structure, is its capacity to bind and transport small lipophilic substancies such as free fatty acids, retinoids, arachidonic acid and various steroids. Although Lipocalin-2 was identified more than a decade ago, the physiologic function of this protein remains poorly understood. LCN2 appears to be upregulated in cells under the “stress” (e.g. from infection, inflammation, in tissues undergoing involution to ischemia or neoplastic transformation). Plasma levels of LCN2 rise in inflammatory or infective condition. It mediates an immune response to bacterial infection by sequestering iron. In this case, LCN2 may represent a promising candidate as a therapeutic agent against bacterial infection. Several recent reports suggest that LCN2 might represent a sensitive biomarker for early renal injury. In cardiopulmonary bypass-induced acute renal injury and cisplatin-induced nephrotoxic injury, increased de novo synthesis of LCN2 in proximal tubule cells leads to sharply increased concentration of this protein in both urine and serum. LCN2 might also be critical for normal kidney formation in the earliest stages of mammalian development. LCN2 may play an important role in breast cancer, in complex with MMP-9, by protecting MMP-9 from degradation thereby enhancing its enzymatic activity and facilitating angiogenesis and tumor growth. LCN2 is also highly expressed after malignant transformation of the lung, colon and pancreatic epithelia. Circulating levels of LCN2 play a causative role in pathogenesis of obesity-induced metabolic disorders such as insulin resistance, Type 2 Diabetes Mellitus and cardiovascular disorders. In addition, serum LCN2 concentrations were positively associated with adipocyte-fatty acid binding protein (A-FABP), a novel serum marker for adiposity and metabolic syndrome.

Areas of investigation: Bacterial infection Renal injury Angiogenesis Oncology Diabetes mellitus Metabolic syndrome Features

References to Product

References

  • Almalki WH, Abdalla AN, Elkeraie AF, Abdelhadi AM, Elrggal M, Elrggal ME. Effect of fluorescein angiography on renal functions in type 2 diabetes patients: A pilot study. Saudi J Kidney Dis Transpl. 2017 May-Jun;28(3):491-498
  • Baumert M, Surmiak P, ORCID: http://orcid.org/0000-0003-3662-634X, Wiecek A, Walencka Z. Serum NGAL and copeptin levels as predictors of acute kidney injury in asphyxiated neonates. Clin Exp Nephrol. 2016 Sep 2;
  • Bayram A,Esmaoglu A, Akin A,Baskol G, Aksu R, Bicer C, Demirtas A, Mutluay R, Boyaci A. The effects of intraoperative infusion of dexmedetomidine on early renal function after percutaneous nephrolithotomy. Acta Anaesthesiologica Scandin. 2011; 55; (539–544)
  • Borkham-Kamphorst E, van de Leur E, Zimmermann HW, Karlmark KR, Tihaa L, Haas U, Tacke F, Berger T, Mak TW, Weiskirchen R. Protective effects of lipocalin-2 (LCN2) in acute liver injury suggest a novel function in liver homeostasis. Biochim Biophys Acta. 2013 May;1832 (5):660-73
  • Corripio R, Gonzalez-Clemente JM, Perez-Sanchez J, Naf S, Gallart L, Nosas R, Vendrell J, Caixas A. Weight loss in prepubertal obese children is associated with a decrease in adipocyte fatty-acid-binding protein without changes in lipocalin-2: a 2-year longitudinal study. Eur J Endocrinol. 2010 Dec;163 (6):887-93
  • El-Gamasy MA, El-Naghy W. Urinary Neutrophil Gelatinase-Associated Lipocalin and Urinary Soluble CXCL16 as Biomarkers of Activity in Pediatric Lupus Nephritis. Indian J Nephrol. 2018;28(6):427-432.
  • Gombert A, Prior I, Martin L, et al. Urine neutrophil gelatinase-associated lipocalin predicts outcome and renal failure in open and endovascular thoracic abdominal aortic aneurysm surgery. Sci Rep. 2018;8(1):12676. Published 2018 Aug 23. doi:10.1038/s41598-018-31183-1
  • Chen HH, Lan YF, Li HF, Cheng CF, Lai PF, Li WH, Lin H. Urinary miR-16 transactivated by C/EBPbeta reduces kidney function after ischemia/reperfusion-induced injury. Sci Rep. 2016;6:27945
  • Issa VS, Andrade L, Ayub-Ferreira SM, Bacal F, de Braganca AC, Guimaraes GV, Marcondes-Braga FG, Cruz FD, Chizzola PR, Conceicao-Souza GE, Velasco IT, Bocchi EA. Hypertonic saline solution for prevention of renal dysfunction in patients with decompensated heart failure. Int J Cardiol. 2013 Jul 15;167 (1):34-40
  • Kiskac M, Zorlu M, Akkoyunlu ME, Kilic E, Karatoprak C, Cakirca M, Yavuz E, Ardic C, Camli AA, Cikrikcioglu M, Kart L. Vaspin and lipocalin-2 levels in severe obsructive sleep apnea. J Thorac Dis. 2014 Jun;6 (6):720-5
  • Kraydaschenko O, Berezin A, Dolinnaya M. Serum Cystatin C and Neutrophil Gelatinase-Associated Lipocalin as Biomarkers of Glomerular and Tubular Kidney Damage in Patients with Chronic Glomerulonephritis and Saved Renal Function. Biological Markers and Guided . July 2016;3 (1):147-154
  • Luque-Ramirez M, Martinez-Garcia MA, Montes-Nieto R, Fernandez-Duran E, Insenser M, Alpanes M, Escobar-Morreale HF. Sexual dimorphism in adipose tissue function as evidenced by circulating adipokine concentrations in the fasting state and after an oral glucose challenge. Hum Reprod. 2013 Jul;28 (7):1908-18
  • Mady GAS, Nabi AAAA, Shawky SM, Baki AH, Elgaaly SA. Urinary Neutrophil Gelatinase Associated Lipocalcin (uNGA|L) as an early marker for contrast induced acute kidney injury after cardiac catheterization. International Journal of Advan. 2016;3 (6)
  • Mohamed WA, Schaalan MF. Antidiabetic efficacy of lactoferrin in type 2 diabetic pediatrics; controlling impact on PPAR-γ, SIRT-1, and TLR4 downstream signaling pathway. Diabetol Metab Syndr. 2018;10:89. Published 2018 Dec 4. doi:10.1186/s13098-018-0390-x
  • Morieri ML, Guardigni V, Sanz JM, et al. Adipokines levels in HIV infected patients: lipocalin-2 and fatty acid binding protein-4 as possible markers of HIV and antiretroviral therapy-related adipose tissue inflammation. BMC Infect Dis. 2018;18(1):10. Published 2018 Jan 5. doi:10.1186/s12879-017-2925-4
  • Ni X, Gu Y, Yu H, et al. Serum Adipocyte Fatty Acid-Binding Protein 4 Levels Are Independently Associated with Radioisotope Glomerular Filtration Rate in Type 2 Diabetic Patients with Early Diabetic Nephropathy. Biomed Res Int. 2018;2018:4578140. Published 2018 May 27. doi:10.1155/2018/4578140
  • Panidis D, Tziomalos K, Koiou E, Kandaraki EA, Tsourdi E, Delkos D, Kalaitzakis E, Katsikis I. The effects of obesity and polycystic ovary syndrome on serum lipocalin-2 levels: a cross-sectional study. Reprod Biol Endocrinol. 2010;8:151
  • Patel ML, Sachan R, Shyam R, Kumar S, Kamal R, Misra A. Diagnostic accuracy of urinary neutrophil gelatinase-associated lipocalin in patients with septic acute kidney injury. International Journal of Nephr. July 2016;:161-169
  • Romanidou G, Konstantinidis TG, Koutsogiannis O, et al. Study of Antiphospholipid Antibodies in Patients with Arterial Hypertension. Med Sci (Basel). 2018;6(4):102. Published 2018 Nov 13. doi:10.3390/medsci6040102
  • Roth GA, Nickl S, Lebherz-Eichinger D, Schmidt EM, Ankersmit HJ, Faybik P, Hetz H, Krenn CG. Lipocalin-2 serum levels are increased in acute hepatic failure. Transplant Proc. 2013 Jan-Feb;45 (1):241-4
  • Santana-Santos E, Gowdak LH, Gaiotto FA, Puig LB, Hajjar LA, Zeferino SP, Drager LF, Shimizu MH, Bortolotto LA, De Lima JJ. High Dose of N-Acetylcystein Prevents Acute Kidney Injury in Chronic Kidney Disease Patients Undergoing Myocardial Revascularization. Ann Thorac Surg. 2014 Mar 20;
  • Scheer FA, Chan JL, Fargnoli J, Chamberland J, Arampatzi K, Shea SA, Blackburn GL, Mantzoros CS. Day/night variations of high-molecular-weight adiponectin and lipocalin-2 in healthy men studied under fed and fasted conditions. Diabetologia. 2010 Nov;53 (11):2401-5
  • Sigdel KR, Duan L, Wang Y, Hu W, Wang N, Sun Q, Liu Q, Liu X, Hou X, Cheng A, Shi G, Zhang Y. Serum Cytokines Th1, Th2 and Th17 Expression Profiling In Active Lupus Nephritis-IV: From a Southern Chinese Han Population.
  • Sporek M, Gala-Błądzińska A, Dumnicka P, Mazur-Laskowska M, Kielczewski S,Walocha J, Ceranowicz P, Kuźniewski M, Mituś J, Kuśnierz-Cabala B. Urine NGAL is useful in the clinical evaluation of renal function in the early course of acute pancreatitis. Folia Med Cracov. 2016;56(1):13-25.
  • Stejskal D, Karpisek M, Humenanska V, Hanulova Z, Stejskal P, Kusnierova P, Petzel M. Lipocalin-2: development, analytical characterization, and clinical testing of a new ELISA. Horm Metab Res. 2008 Jun;40 (6):381-5
  • Tyagi A, Lahan S, Verma G, Das S, Kumar M. Role of Intra-abdominal Pressure in Early Acute Kidney Injury: A Prospective Cohort Study in Critically Ill Obstetric Patients. Indian J Crit Care Med. 2018;22(8):602-607.
  • Tyagi A, Verma G, Luthra A, et al. Risk of early postoperative acute kidney injury with stroke volume variation-guided tetrastarch versus Ringer's lactate. Saudi J Anaesth. 2019;13(1):9-15.
  • Vesnina Z V, Lishmanov Y B, Alexandrova E A, Nesterov E A. Evaluation of Nephroprotective Efficacy of Hypoxic Preconditioning in Patients Undergoing Coronary Artery Bypass Surgery. Cardiorenal Medicine. 2016;6 (4)
  • Yavuz S, Anarat A, Acarturk S, Dalay AC, Kesiktas E, Yavuz M, Acarturk TO. Neutrophil gelatinase associated lipocalin as an indicator of acute kidney injury and inflammation in burned children. Burns. 2014 Jun;40 (4):648-54
  • Zorlu M, Kiskac M, Karatoprak C, Kesgin S, Cakirca M, Yildiz K, Ardic C, Cikrikcioglu MA, Erkoc R. Assessment of serum apelin and lipocalin-2 levels in patients with subclinical hypothyroidism. Ann Endocrinol (Paris). 2014 Feb;75 (1):10-4
  • Żyłka A, Dumnicka P, Kuśnierz-Cabala B, et al. Markers of Glomerular and Tubular Damage in the Early Stage of Kidney Disease in Type 2 Diabetic Patients. Mediators Inflamm. 2018;2018:7659243. Published 2018 Aug 9. doi:10.1155/2018/7659243
References to Summary

References to Lipocalin-2/NGAL

  • Bachorzewska-Gajewska H, Malyszko J, Sitniewska E, Malyszko JS, Dobrzycki S. Neutrophil gelatinase-associated lipocalin (NGAL) correlations with cystatin C, serum creatinine and eGFR in patients with normal serum creatinine undergoing coronary angiography. Nephrol Dial Transplant. 2007 Jan;22 (1):295-6
  • Bachorzewska-Gajewska H, Malyszko J, Sitniewska E, Malyszko JS, Poniatowski B, Pawlak K, Dobrzycki S. NGAL (neutrophil gelatinase-associated lipocalin) and cystatin C: are they good predictors of contrast nephropathy after percutaneous coronary interventions in patients with stable angina and normal serum creatinine?. Int J Cardiol. 2008 Jul 4;127 (2):290-1
  • Berger T, Togawa A, Duncan GS, Elia AJ, You-Ten A, Wakeham A, Fong HE, Cheung CC, Mak TW. Lipocalin 2-deficient mice exhibit increased sensitivity to Escherichia coli infection but not to ischemia-reperfusion injury. Proc Natl Acad Sci U S A. 2006 Feb 7;103 (6):1834-9
  • Brunner HI, Mueller M, Rutherford C, Passo MH, Witte D, Grom A, Mishra J, Devarajan P. Urinary neutrophil gelatinase-associated lipocalin as a biomarker of nephritis in childhood-onset systemic lupus erythematosus. Arthritis Rheum. 2006 Aug;54 (8):2577-84
  • Flo TH, Smith KD, Sato S, Rodriguez DJ, Holmes MA, Strong RK, Akira S, Aderem A. Lipocalin 2 mediates an innate immune response to bacterial infection by sequestrating iron. Nature. 2004 Dec 16;432 (7019):917-21
  • Lin HH, Li WW, Lee YC, Chu ST. Apoptosis induced by uterine 24p3 protein in endometrial carcinoma cell line. Toxicology. 2007 May 20;234 (3):203-15
  • Miharada K, Hiroyama T, Sudo K, Nagasawa T, Nakamura Y. Lipocalin 2 functions as a negative regulator of red blood cell production in an autocrine fashion. FASEB J. 2005 Nov;19 (13):1881-3
  • Mishra J, Ma Q, Kelly C, Mitsnefes M, Mori K, Barasch J, Devarajan P. Kidney NGAL is a novel early marker of acute injury following transplantation. Pediatr Nephrol. 2006 Jun;21 (6):856-63
  • Mishra J, Ma Q, Prada A, Mitsnefes M, Zahedi K, Yang J, Barasch J, Devarajan P. Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. J Am Soc Nephrol. 2003 Oct;14 (10):2534-43
  • Mitsnefes MM, Kathman TS, Mishra J, Kartal J, Khoury PR, Nickolas TL, Barasch J, Devarajan P. Serum neutrophil gelatinase-associated lipocalin as a marker of renal function in children with chronic kidney disease. Pediatr Nephrol. 2007 Jan;22 (1):101-8
  • Mori K, Nakao K. Neutrophil gelatinase-associated lipocalin as the real-time indicator of active kidney damage. Kidney Int. 2007 May;71 (10):967-70
  • Nguyen MT, Devarajan P. Biomarkers for the early detection of acute kidney injury. Pediatr Nephrol. 2008 Dec;23 (12):2151-7
  • Parikh CR, Jani A, Mishra J, Ma Q, Kelly C, Barasch J, Edelstein CL, Devarajan P. Urine NGAL and IL-18 are predictive biomarkers for delayed graft function following kidney transplantation. Am J Transplant. 2006 Jul;6 (7):1639-45
  • Trachtman H, Christen E, Cnaan A, Patrick J, Mai V, Mishra J, Jain A, Bullington N, Devarajan P. Urinary neutrophil gelatinase-associated lipocalcin in D+HUS: a novel marker of renal injury. Pediatr Nephrol. 2006 Jul;21 (7):989-94
  • Vaidya VS, Ramirez V, Ichimura T, Bobadilla NA, Bonventre JV. Urinary kidney injury molecule-1: a sensitive quantitative biomarker for early detection of kidney tubular injury. Am J Physiol Renal Physiol. 2006 Feb;290 (2):F517-29
  • van Dam RM, Hu FB. Lipocalins and insulin resistance: etiological role of retinol-binding protein 4 and lipocalin-2?. Clin Chem. 2007 Jan;53 (1):5-7
  • Wang Y, Lam KS, Kraegen EW, Sweeney G, Zhang J, Tso AW, Chow WS, Wat NM, Xu JY, Hoo RL, Xu A. Lipocalin-2 is an inflammatory marker closely associated with obesity, insulin resistance, and hyperglycemia in humans. Clin Chem. 2007 Jan;53 (1):34-41
Related Products Documents