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microRNA IN A NEW LIGHT!

MicroRNAs (miRNAs) are new and promising potential biomarkers for diagnosis and prognosis of many diseases, which opens up a new field of goals and challenges for the BioVendor R&D team. Development of miRNA diagnostic assays is just the first step; our mission is to provide validated kits to measure individual miRNAs or diagnostically relevant miRNA “groups” supported with tools for standardized miRNA isolation, sample collection, and pretreatment.

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MicroRNAs (miRNAs) are small, non-coding RNA molecules typically containing ~22 nucleotides found in human, animals, plants and some viruses which are playing an important regulatory role in the gene translation via silencing or degradation of target mRNAs. During the RNA silencing, miRNA functions as a complementary guide which binds with its target mRNAs, followed by action of AGO proteins family which works as effectors by recruiting factors that induce translational repression, mRNA deadenylation and mRNA decay. (Minju Ha, Nature Reviews, 2014)

MicroRNAs (miRNAs) are involved in virtually all physiologic processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation. The biogenesis and function of miRNAs are tightly regulated, and their dysregulation is often associated with pathology of many diseases such as cancer, heart disease or neurological disorders. Recently, the miRNA has been proposed as powerful biomarker for prediction of the diseases, treatment response or prediction of progression.

 

miRNA Quantification Method

miRNA Quantification Method

miREIA – miRNA enzyme immunoassay

Novel method for miRNA quantification based on immunoassay. The novel method involves hybridization of miRNA isolated from patient sample to complementary biotinylated DNA oligonucleotide probe followed by monoclonal antibody detection of perfectly matched DNA/miRNA hybrids in several different arrangements and possible methods of visualization:

  • Colorimetric – miReia – miRNA enzyme immunoassay
  • Chemiluminescent – miRacle – miRNA antibody/capture luminometry


Click to see flyer about miRNA quantification

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miREIA (miRNA Enzyme Immunoassay) – a novel, immunoassay-based method of miRNA quantification

miRNA isolated from a patient sample is hybridized to complementary biotinylated DNA oligonucleotide probe.

The DNA/RNA hybrids are then processed in the manner of ELISA in a microtiter plate coated with a monoclonal antibody specific to perfectly matched DNA/miRNA hybrids.

After washing, the solid phase is incubated with streptavidin-HRP conjugate and the resulting complexes are visualized (after another washing step) by a chromogenic substrate.

The miREIA exhibits superior analytical specificity, limit of detection as low as 0.1 attomol/μl miRNA, excellent analytical characteristics and strong correlation with the qRT-PCR method (Pearson correlation coefficient >0.9).

miREIA can be run on common immunoassay analyzers, is compatible with standard clinical workflow, does not require amplification steps and results are obtained in less than three hours including miRNA profiling.


The assay arrangement:

  • Classical ELISA format
  • Array format
  • Luminex beads format

 

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miREIA vs. RT-qPCR

miREIA vs. RT-qPCR


miREIA RT-qPCR
Reverse transcription reaction NOT required Required
Amplification steps NOT required Required
Total time to result 2 hours  >3 hours
Quantification Absolute (attomol/μl) Relative
Instrumentation required Immunoassay equipment  Specific PCR cycler
Cost per sample Lower Higher

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Scientific Presentations

Scientific Presentations

Posters presented at scientific congresses

May 2018:
EAS 2018 (Lisbon, Portugal)
- poster Novel immunoassay approach to investigate microRNA biomarkers in acute myocardial infartion

April 2018:
AACR 2018 (Chicago, USA) - poster New clinical diagnostic approach for miRNA quantification using the Chlorella virus DNA ligase

May/June 2017:
RNA society congress 2017 (Prague, Czech Republic) - poster miRiam – NEW TRENDS IN microRNA QUANTIFICATION

April 2017:
85th EAS congress 2017 (Prague, Czech Republic) - e-poster MicroRNA-BASED DIAGNOSTICS IN CARDIOVASCULAR DISEASE

October 2016:
GTCbio: Biomarker Summit Europe 2016 - 5th Biomarkers in Diagnostics Conference (Berlin, Germany) - poster miRiam – THE NEW INSIGHT INTO miRNA QUANTIFICATION

  

Do you want to know more?

Click to download the poster on microRNA and Cardiovascular Disease.

 

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