Sandwich ELISA, Biotin-labelled antibody
Cell lysate, Cell culture supernatant
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
15.6 - 1000 pg/ml
Limit of Detection
n = 8
CV = 7.2%
n = 5
CV = 8.6%
It is intended for research use only
The total assay time is less than 4 hours
The kit measures mouse gasdermin D in cell cuture supernatant and cell lysate
Assay format is 96 wells
Components of the kit are provided ready to use, concentrated or dried
Immune Response, Infection and Inflammation
Gasdermin D forming pores regulate the non-conventional secretion of cytokines such as IL-1β, in response to cytosolic LPS and other activators of the inflammasome. Neutrophil extrusion of neutrophil extracellular traps (NETs) and concomitant cell death (NETosis), a particular neutrophil defense against pathogens, are dependent on Gasdermin D. Gasdermin D-mediated pyroptosis is regulated at the level of lipid peroxidation and seems to be a key effector in the LPS-induced lethal sepsis.