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Human Parathyroid Hormone (PTH) ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:PTH
  • Species:Human
Cat. No. Size Price

RIS003R 96 wells (1 kit) $465,15
PubMed Product Details
Technical Data


Sandwich ELISA, HRP-labelled antibody


Immunoenzymetric assay for the in vitro quantitative measurement of human Intact Parathyroid Hormone (PTH) in serum and plasma.


Serum, Plasma

Sample Requirements

200 µl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

22–1400 pg/ml

Limit of Detection

0.8 pg/ml

Intra-assay (Within-Run)

CV < 4% (serum)

Inter-assay (Run-to-Run)

CV < 5% (serum)

Spiking Recovery

serum 95%, heparin plasma 96%, EDTA plasma 100%



  • RUO
  • assay for the in vitro quantitative measurement of human Intact Parathyroid Hormone (PTH) in serum and plasma
  • limit of detection 0.8 pg/ml

Research topic

Bone and cartilage metabolism, Oncology


Human parathyroid hormone (hPTH) is a major physiological regulator of phosphocalcic metabolism. hPTH increases serum calcium concentrations by its actions on kidney (enhancing tubular Ca++ reabsorption and phosphate excretion) and bone (stimulating osteoclastic activity and bone resorption). It indirectly affects intestinal absorption of Ca++ by stimulating renal 1αhydroxylation of 25 hydroxyvitamin D. The release of PTH is controlled in a negative feedback loop by the serum concentration of Ca++. PTH is synthesized in the chief cells of the parathyroid glands and secreted as an 84 amino acid molecule called "intact PTH", which is the main bioactive product. This molecule is degraded by proteolytic cleavage between amino acids 33-37 at peripheral sites to form biologically active amino- terminal fragments and biologically inactive carboxyl-terminal fragments. The carboxylterminal fragments are cleared only by glomerular filtration, while the bioactive intact PTH and amino-terminal fragments are also metabolically degraded in the liver and other tissues. The halflife of the carboxyl- terminal fragments increases dramatically in patients with renal failure. Thus, the measurement of intact PTH correlates best with the hormone production and biological activity.

Clinical application The measurement of intact hPTH is used to establish the diagnosis of primary hyperparathyroidism by demonstrating elevated serum levels of bioactive PTH. It allows documenting the occurrence of secondary hyperparathyroidism in patients with Vit.D deficiency, intestinal malabsorption, or renal failure. In this last situation, the absence of interference with the inactive carboxyl-terminal fragments is especially valuable. The specificity and high sensitivity of the assay also allows differentiating clearly low serum PTH levels in hypoparathyroidism or in tumor-induced hypercalcaemia.

Product References (3)


  • Varamenti E, Cherif A, Nikolovski Z, Tabben M, Jamurtas AZ, Cardinale M. Exploring possible relationships between 25(OH)D deficiency and variables related to inflammation, endothelial function, and enzymatic antioxidants in adolescent athletes: a prospective study. Biol Sport. 2019 Jun;36(2):113-118. doi: 10.5114/biolsport.2019.81112. Epub 2019 Jan 11. PubMed PMID: 31223187. PubMed CentralPMCID: PMC6561226. See more on PubMed
  • Chang WT, Wu CH, Hsu LW, Chen PW, Yu JR, Chang CS, Tsai WC, Liu PY. Serum vitamin D, intact parathyroid hormone, and Fetuin A concentrations were associated with geriatric sarcopenia and cardiac hypertrophy. Sci Rep. 2017 Jan 23;7:40996. doi: 10.1038/srep40996. PubMed PMID: 28112206. PubMed CentralPMCID: PMC5253676. See more on PubMed
  • Furlan M. [Von Willebrand's disease]. Schweiz Med Wochenschr. 1987 Nov 14;117(46):1798-806. PubMed PMID: 3122318. See more on PubMed
Summary References (6)

References to Parathyroid Hormone

  • Bouillon R, Coopmans W, Degroote DE, Radoux D, Eliard PH. Immunoradiometric assay of parathyrin with polyclonal and monoclonal region-specific antibodies. Clin Chem. 1990 Feb;36 (2):271-6
  • Goltzman D, Henderson B, Loveridge N. Cytochemical bioassay of parathyroid hormone: characteristics of the assay and analysis of circulating hormonal forms. J Clin Invest. 1980 Jun;65 (6):1309-17
  • Hackeng WH, Lips P, Netelenbos JC, Lips CJ. Clinical implications of estimation of intact parathyroid hormone (PTH) versus total immunoreactive PTH in normal subjects and hyperparathyroid patients. J Clin Endocrinol Metab. 1986 Aug;63 (2):447-53
  • Martin KJ, Hruska KA, Freitag JJ, Klahr S, Slatopolsky E. The peripheral metabolism of parathyroid hormone. N Engl J Med. 1979 Nov 15;301 (20):1092-8
  • Potts JT Jr, Kronenberg HM, Habener JF, Rich A. Biosynthesis of parathyroid hormone. Ann N Y Acad Sci. 1980;343:38-55
  • Potts JT Jr, Kronenberg HM, Rosenblatt M. Parathyroid hormone: chemistry, biosynthesis, and mode of action. Adv Protein Chem. 1982;35:323-96
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