Total 370 AA. MW: 41.0 kDa (calculated). UniProtKB acc. No. P26022 (Glu18–Ser381). C-terminal His-tag (6 extra AA). Protein identity confirmed by MS.
Amino Acid Sequence
˃ 90 % by SDS-PAGE
12 % SDS-PAGE separation of Human Pentraxin 3 (HEK):
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. Reduced and boiled sample, 2.5 μg/lane
3. Non-reduced and non-boiled sample, 2.5 μg/lane
< 1.0 EU/µg
Filtered (0.4 μm) and lyophilized from 0.5 mg/mL solution in phosphate buffered saline, pH 7.4
Add 200 µL of deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely.
Western blotting, ELISA
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at -80 °C. Lyophilized protein remains stable until the expiry date when stored at -80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein. Endotoxin level determination.
This product is intended for research use only.