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PMN Elastase Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:Elastase-2, Leukocyte elastase, Neutrophil elastase
  • Species:Human
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Cat. No. Size Price


RM191021100 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Plasma-EDTA, Plasma-Citrate, Cerebrospinal fluid, Bronchoalveolar lavage, Seminal plasma

Sample Requirements

10 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at 2–8°C. Storage conditions for aliquots of Standards, Quality Controls and samples are – 20°C. Shelf life of components is 30 days after opening or until the expiration date.

Calibration Curve

Calibration Range

15.6–1000 ng/ml

Limit of Detection

0.2 ng/ml

Intra-assay (Within-Run)

n = 10; CV = 4.8%

Inter-assay (Run-to-Run)

n = 5; CV = 5.6%

Spiking Recovery

104,30%

Dilutation Linearity

106,80%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • human Yes
  • monkey Yes (recommended dilution 1:3)
Summary

Features

  • European Union: for in vitro diagnostic use
  • Rest of the world: for research use only!
  • The total assay time is less than 3 hours.
  • The kit measures PMN elastase in plasma (EDTA and citrate), exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma.
  • Assay format is 96 wells.
  • Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Immune Response, Infection and Inflammation, Animal studies

Summary

The human organism reacts with an inflammatory response to attacks of invading pathogens (micro-organisms and viruses) or damaged tissue (after accidents or surgery). Polymorphonuclear (PMN) granulocytes play an important role as primary defence cells in this inflammatory reaction. Different bloodstream mediators (cytokines, leukotrienes, complement factors, bacterial endotoxins, clotting and fibrinolysis factors) attract and stimulate these cells to phagocytize and destroy not naturally occurring agents. PMN granulocytes use proteinases to digest these agents and tissue debris. One of these proteinases is PMN elastase which is localised in the azurophilic granules of the polymorphonuclear granulocytes. During phagocytosis of foreign substances these enzymes are also partially excreted into the extracellular surrounding, where the aktivity of PMN elastase is regulated by inhibitors (esp. the α1-proteinase inhibitor, α1-PI). An overwhelming release of PMN elastase, however, can exceed the inhibitory potential of the α1-proteinase inhibitor. Thus, enzymatically active PMN elastase, together with simultaneously produced oxidants (O2-radicals, H2O2, OH-radicals), can cause local tissue injury. Due to the bloodstream and lymphatic system, however, α1-PI is delivered subsequently and eventually able to form a complex with all excreted elastase. Therefore, the concentration of the PMN elastase/ α1-PI complex correlates with the released PMN elastase and can be used as a measure for the activity of granulocytes during an inflammatory response. Primarily, determinations of PMN elastase find its application in observation of the course of trauma, shock and sepsis. Further indications are the areas of hemodialysis, infections by obstetrics, joint diseases, effusions of sport injuries, intestinal affection, pancreatitis, cystic fibrosis and male adnex affections.

References to Product

References

  • Allan EK, Holyoake TL, Jorgensen HG. .
  • Chimenti L, Morici G, Paterno A, Bonanno A, Vultaggio M, Bellia V, Bonsignore MR. Environmental conditions, air pollutants, and airway cells in runners: a longitudinal field study. J Sports Sci. 2009 Jul;27 (9):925-35
  • Laing SJ, Jackson AR, Walters R, Lloyd-Jones E, Whitham M, Maassen N, Walsh NP. Human blood neutrophil responses to prolonged exercise with and without a thermal clamp. J Appl Physiol. 2008 Jan;104 (1):20-6
  • Lisowska-Myjak B, Zytynska-Daniluk J, Skarzynska E. Concentrations of neutrophil-derived proteins in meconium and their correlations. Biomark Med. 2016 Aug;10 (8):819-29
  • Naskalski JW, Kapusta M, Fedak D, Dumnicka P, Kusnierz-Cabala B, Kuzniewski M, Sulowicz W. Effect of Hemodialysis on Acid Leukocyte-Type Ribonuclease, Alkaline Ribonuclease and Polymorphonuclear Elastase Serum Levels in Patients with End-Stage Renal Disease. Nephron Clin Pract. 2009 Jun 16;112 (4):c248-c254
  • Park CJ, Clark SG, Lichtensteiger CA, Jamison RD, Johnson AJ. Accelerated wound closure of pressure ulcers in aged mice by chitosan scaffolds with and without bFGF. Acta Biomater. 2009 Jul;5 (6):1926-36
  • Pawlica-Gosiewska D, Solnica B, Gawlik K, Cibor D, Mach T, Fedak D, Owczarek D. The use of selected neutrophil protein plasma concentrations in the diagnosis of Crohn's disease and ulcerative colitis - a preliminary report. Postepy Hig Med Dosw (Online). 2017 Apr 6;71(0):243-253
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