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Pregnenolone ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Species:Human
Cat. No. Size Price

RCD022R 96 wells (1 kit) $467,44
PubMed Product Details
Technical Data


Competitive ELISA, Immobilized antigen


For the direct quantitative determination of Pregnenolone by enzyme immunoassay in human serum. For research use only.



Sample Requirements

50 μl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.1–25.6 ng/ml

Limit of Detection

0.054 ng/ml

Intra-assay (Within-Run)

CV = 9.2%

Inter-assay (Run-to-Run)

CV = 10%



  • RUO
  • calibration range 0.1-25.6 ng/ml
  • limit of detection 0.054 ngml
  • controls
  • intra-assay CV = 9.2%
  • inter-assay CV = 10%

Research topic

Steroid hormones


Pregnenolone (3β-hydroxypregn-5-en-20-one) is the first steroid to be derived from cholesterol in the pathway of steroidogenesis, and it is the common precursor for all of the adrenal and gonadal steroids. Its production occurs in the mitochondrion by cleavage of the C-20 side chain of cholesterol by the P-450SCC enzyme. Once produced, Pregnenolone may be utilized by two pathways of steroidogenesis. Pregnenolone may either be converted to 17-OH Pregnenolone via the enzymatic action of 17α-hydroxylase or to progesterone via the enzymatic action of 3β-hydroxysteroid dehydrogenase. Elevated Pregnenolone levels occur in forms of congenital adrenal hyperplasia (CAH), due to 3β-hydroxysteroid dehydrogenase deficiency or 17α-hydroxylase deficiencies. Higher levels have also been reported in women with idiopathic hirsutism. Studies on Pregnenolone levels in regard to sex and age differences indicate that maximum levels occur at approximately 17 and 16 years of age for women and men, while minimum levels occur at approximately 37 and 38 years of age for women and men, respectively. In general, women were found to have slightly higher values when compared to men. Many areas of Pregnenolone physiology remain to be investigated. Current research indicates that the determination of Pregnenolone in serum may be useful for studying its metabolite, Pregnenolone sulfate, which has been reported to have various effects in the mammalian brain and central nervous system. Example

Summary References (10)

References to Pregnenolone

  • Abraham GE, Buster JE, Kyle FW, Corrales PC, Teller RC. Radioimmunoassay of plasma pregnenolone. J Clin Endocrinol Metab. 1973 Jul;37 (1):40-5
  • Akwa Y, Young J, Kabbadj K, Sancho MJ, Zucman D, Vourc'h C, Jung-Testas I, Hu ZY, Le Goascogne C, Jo DH, et al. Neurosteroids: biosynthesis, metabolism and function of pregnenolone and dehydroepiandrosterone in the brain. J Steroid Biochem Mol Biol. 1991;40 (1-3):71-81
  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • Hill M, Lukac D, Lapcik O, Sulcova J, Hampl R, Pouzar V, Starka L. Age relationships and sex differences in serum levels of pregnenolone and 17-hydroxypregnenolone in healthy subjects. Clin Chem Lab Med. 1999 Apr;37 (4):439-47
  • McKenna TJ, Brown RD. Pregnenolone in man: plasma levels in states of normal and abnormal steroidogenesis. J Clin Endocrinol Metab. 1974 Mar;38 (3):480-5
  • McKenna TJ, Jennings AS, Liddle GW, Burr IM. Pregnenolone, 17-OH-pregnenolone, and testosterone in plasma of patients with congenital adrenal hyperplasia. J Clin Endocrinol Metab. 1976 May;42 (5):918-25
  • McKenna TJ, Miller RB, Liddle GW. Plasma pregnenolone and 17-OH-pregnenolone in patients with adrenal tumors, ACTH excess, or idiopathic hirsutism. J Clin Endocrinol Metab. 1977 Feb;44 (2):231-6
  • Robel P, Young J, Corpechot C, Mayo W, Perche F, Haug M, Simon H, Baulieu EE. Biosynthesis and assay of neurosteroids in rats and mice: functional correlates. J Steroid Biochem Mol Biol. 1995 Jun;53 (1-6):355-60
  • Weusten JJ, Smals AG, Hofman JA, Kloppenborg PW, Benraad TJ. Early time sequence in pregnenolone metabolism to testosterone in homogenates of human and rat testis. Endocrinology. 1987 May;120 (5):1909-13
  • Wilson JD, Foster DW. Textbook of Endocrinology 8th Edition. W.B. Saunders Company. pp 495-497, 1992
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