Select country set
Menu Shopping cart 0,00 Search
Manufactured by BioVendor

Pregnenolone ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Species:Human
Please select your region to see available products and prices.
Cat. No. Size Price

RCD022R 96 wells (1 kit)
PubMed Product Details
Technical Data


Competitive ELISA, Immobilized antigen



Sample Requirements

50 μl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.1–25 ng/ml

Limit of Detection

0.05 ng/ml


Research topic

Steroid hormones


Pregnenolone is the first steroid to be derived from cholesterol in the pathway of steroidogenesis, and it is the common precursor for all of the adrenal and gonadal steroids. Its production occurs in the mitochondrion by cleavage of the C-20 side chain of cholesterol by the P-450SCC enzyme. Elevated pregnenolone levels occur in forms of congenital adrenal hyperplasia. The determination of pregnenolone in serum may be useful for studying its metabolite, pregnenolone sulfate, which has been reported to have various effects in the mammalian brain and central nervous system.

Summary References (10)

References to Pregnenolone

  • Abraham GE, Buster JE, Kyle FW, Corrales PC, Teller RC. Radioimmunoassay of plasma pregnenolone. J Clin Endocrinol Metab. 1973 Jul;37 (1):40-5
  • Akwa Y, Young J, Kabbadj K, Sancho MJ, Zucman D, Vourc'h C, Jung-Testas I, Hu ZY, Le Goascogne C, Jo DH, et al. Neurosteroids: biosynthesis, metabolism and function of pregnenolone and dehydroepiandrosterone in the brain. J Steroid Biochem Mol Biol. 1991;40 (1-3):71-81
  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • Hill M, Lukac D, Lapcik O, Sulcova J, Hampl R, Pouzar V, Starka L. Age relationships and sex differences in serum levels of pregnenolone and 17-hydroxypregnenolone in healthy subjects. Clin Chem Lab Med. 1999 Apr;37 (4):439-47
  • McKenna TJ, Brown RD. Pregnenolone in man: plasma levels in states of normal and abnormal steroidogenesis. J Clin Endocrinol Metab. 1974 Mar;38 (3):480-5
  • McKenna TJ, Jennings AS, Liddle GW, Burr IM. Pregnenolone, 17-OH-pregnenolone, and testosterone in plasma of patients with congenital adrenal hyperplasia. J Clin Endocrinol Metab. 1976 May;42 (5):918-25
  • McKenna TJ, Miller RB, Liddle GW. Plasma pregnenolone and 17-OH-pregnenolone in patients with adrenal tumors, ACTH excess, or idiopathic hirsutism. J Clin Endocrinol Metab. 1977 Feb;44 (2):231-6
  • Robel P, Young J, Corpechot C, Mayo W, Perche F, Haug M, Simon H, Baulieu EE. Biosynthesis and assay of neurosteroids in rats and mice: functional correlates. J Steroid Biochem Mol Biol. 1995 Jun;53 (1-6):355-60
  • Weusten JJ, Smals AG, Hofman JA, Kloppenborg PW, Benraad TJ. Early time sequence in pregnenolone metabolism to testosterone in homogenates of human and rat testis. Endocrinology. 1987 May;120 (5):1909-13
  • Wilson JD, Foster DW. Textbook of Endocrinology 8th Edition. W.B. Saunders Company. pp 495-497, 1992
Related Products Docs